Antimicrobial Activity of Phytosphingosine Nanoemulsions against Bacteria and Yeasts

Year 2018, Volume: 14 Issue: 2, 223 - 228, 30.06.2018

Yücel Başpınar , Mustafa Kotmakçı İsmail Öztürk

https://doi.org/10.18466/cbayarfbe.403152

Cited By: 4

Abstract

Phytosphingosine
(PS) is a natural antimicrobial ingredient present in the mammalian stratum
corneum of the skin. The skin surface is the first line defense system against
microbial colonization and infections. The aim of this study was to investigate
in vitro antimicrobial effects of PS
and PS-hydrochloride (PSHCl) nanoemulsion (NE) formulations and solutions
against bacteria and fungi. Minimum inhibitory concentrations (MICs) of the
PS-NE and PSHCl-NE formulations and PS/PSHCl solutions were determined by
microdilution method against Gram-positive/Gram-negative bacteria and yeasts.
Inhibitory effects were also examined by disc diffusion method and inhibition
zone diameters were determined. PS/PSHCl solutions and PS-NE/PSHCl-NE
formulations were prepared in different conditions. Physicochemical properties
of the NEs were characterized and antimicrobial activities were evaluated. In
this study we reported that the PS and PSHCl and NEs had variable antimicrobial
activities for a variety of Gram-positive bacteria (MIC range 1 µg/ml to 1024
µg/ml), Gram-negative bacteria (MIC range 8 µg/ml to 1024 µg/ml) and Candida
strains (MIC range 8 µg/ml to 512 µg/ml). Sphingolipids are part of the natural
defense system of the body. We demonstrated that PS and PSHCl solutions and NEs
have broad antimicrobial activity against bacteria and yeasts. In addition, the
newly developed formulations may have potential for prophylactic and
therapeutic intervention in infections.

Keywords

Phytosphingosine, Phytosphingosine hydrochloride, Nanoemulsions, Antimicrobial activity

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