Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Year 2018, Volume: 14 Issue: 2, 237 - 239, 30.06.2018

Burcu Çetin

https://doi.org/10.18466/cbayarfbe.406873

Cited By: 2

Abstract

Determination of the genetic characters of the plants
obtained using plant tissue culture methods is important. In this study, the genetic
fidelity of the plants, which are obtained by the micropropagation of the shoot
tip explants of the Origanum majorana
L. plant of medicinal and economical value in Murashige and Skoog (MS) medium
which contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneacetic
acid (NAA), 3% sucrose and 0.7% agar, has been investigated by using
random amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtained
as a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among the
micropropagated plants. 

Keywords

Genetic fidelity, Medicinal herb, Micropropagation, Origanum majorana L., RAPD

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