Papain Immobilization on NiFe2O4 Magnetic Nanoparticles Functionalized with Gallic Acid and Microwave Assisted Digestion of Bovine Serum Albumin

Year 2018, Volume: 14 Issue: 4, 449 - 454, 28.12.2018

Nuray Güy

https://doi.org/10.18466/cbayarfbe.453636

Abstract

NiFe₂O₄
magnetic nanoparticles were solvothermally prepared. NiFe₂O₄
magnetic nanoparticles were functionalized with gallic acid and then papain
immobilized on NiFe₂O₄ functionalized with gallic acid. The obtained samples were
characterized and the activities of free and immobilized papain were studied.
Immobilized papain showed higher and more effective activity than free papain. This
immobilized papain retained about 75.5% of its initial activity after 8 weeks
of storage at 4 °C in sodium phosphate buffer (0.1 M, pH 6.5), while the free
trypsin protected 30.5% of its initial activity during the same condition.
Furthermore, the immobilized papain protected approximately 51% of its initial
activity following the times of ten sequential reuse. Finally, the microwave
assisted digestion of bovine serum albumin was carried out for 15 s with
matrix-assisted laser desorption/ionization mass spectrometry by using free and
immobilized papain.

Keywords

NiFe2O4, gallic acid, papain, immobilization, protein digestion

References

• 1. Longobardi, S, Gravagnuolo, A.M, Funari, R, Della Ventura, B, Pane, F, Galano, E, Amoresano, A, Marino, G, Giardina, P, A simple MALDI plate functionalization by Vmh2 hydrophobin for serial multi-enzymatic protein digestions, Analytical and Bioanalytical Chemistry, 2015, 407, 487–496.
• 2. Jiang, B, Yang, K, Zhao, Q, Wu, Q, Liang, Z, Zhang, L, Peng, X, Zhang, Y, Hydrophilic immobilized trypsin reactor with magnetic graphene oxide as support for high efficient proteome digestion, Journal of Chromatography A, 2012, 1254, 8–13.
• 3. Sahoo, B, Sahu, S.K, Bhattacharya, D, Dhara, D, Pramanik, P, A novel approach for efficient immobilization and stabilization of papain on magnetic gold nanocomposites, Colloids and Surfaces B: Biointerfaces, 2013, 101, 280–289. 4. He, J, Wu, M, Feng, X, Shao, X, Cai, W, Immobilization of papain on nanoporous silica, RSC Advances, 2014, 4, 13304–13312.
• 5. Hola, K, Markova, Z, Zoppellaro, G, Tucek, J, Zboril, R, Tailored functionalization of iron oxide nanoparticles for MRI, drug delivery, magnetic separation and immobilization of biosubstances, Biotechnology Advances, 2015, 33, 1162–1176.
• 6. Xia, Y, He, Z, Su, J, Tang, B, Hu, K, Lu, Y, Sun, S, Li, X, Fabrication of magnetically separable NiFe2O4/BiOI nanocomposites with enhanced photocatalytic performance under visible-light irradiation, RSC Advances, 2018, 8, 4284–4294.
• 7. Atacan, K, Çakiroǧlu, B, Özacar, M, Improvement of the stability and activity of immobilized trypsin on modified Fe3O4 magnetic nanoparticles for hydrolysis of bovine serum albumin and its application in the bovine milk, Food Chemistry, 2016, 212, 460–468.
• 8. Kang, B, Vales, T.P, Cho, B.K, Kim, J.K, Kim, H.J, Development of gallic acid-modified hydrogels using interpenetrating chitosan network and evaluation of their antioxidant activity, Molecules, 2017, 22, 1-11.
• 9. Ziyatdinova, G, Kozlova, E, Budnikov, H, Poly(gallic acid)/MWNT-modified electrode for the selective and sensitive voltammetric determination of quercetin in medicinal herbs, Journal of Electroanalytical Chemistry, 2018, 821, 73–81.
• 10. Jiao, Q, Wang, Y, Hao, L, Li, H, Zhao, Y, Synthesis of magnetic nickel ferrite microspheres and their microwave absorbing properties, Chemical Research in Chinese Universities, 2016, 32, 678–681.
• 11. Atacan, K, Özacar, M, Özacar, M, Investigation of antibacterial properties of novel papain immobilized on tannic acid modified Ag/CuFe2O4 magnetic nanoparticles, International Journal of Biological Macromolecules, 2018, 109, 720–731.
• 12. Bradford, M.M, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Analytical Biochemistry, 1976, 72, 248–254.
• 13. Metin, A.Ü, Alver, E, Fibrous polymer-grafted chitosan/clay composite beads as a carrier for immobilization of papain and its usability for mercury elimination, Bioprocess and Biosystems Engineering, 2016, 39, 1137–1149.
• 14. Zheng, H, Ni, Y, Xiang, N, Ma, X, Wan, F, Solvothermal synthesis of octahedral NiFe2O4 nanocrystals and catalytic properties for the reduction of some aromatic nitrocompounds, Materials Chemistry and Physics, 2015, 158, 82–88.
• 15. Kooti, M, Naghdi Sedeh, A, Synthesis and Characterization of NiFe2O4 Magnetic Nanoparticles by Combustion Method, Journal of Materials Science and Technology, 2013, 29, 34–38.
• 16. Sheng, W, Xi, Y, Zhang, L, Ye, T, Zhao, X, Enhanced activity and stability of papain by covalent immobilization on porous magnetic nanoparticles, International Journal of Biological Macromolecules, 2018, 114, 143–148.
• 17. Tümay, S.O, Okutan, E, Sengul, I.F, Özcan, E, Kandemir, H, Doruk, T, Çetin, M, Çoşut, B, Naked-eye fluorescent sensor for Cu(II) based on indole conjugate BODIPY dye, Polyhedron, 2016, 117, 161–171.
• 18. Qiao, J, Kim, J.Y, Wang, Y.Y, Qi, L, Wang, F.Y, Moon, M.H, Trypsin immobilization in ordered porous polymer membranes for effective protein digestion, Analytica Chimica Acta, 2016, 906, 156–164.
• 19. Cao, Y, Wen, L, Svec, F, Tan, T, Lv, Y, Magnetic AuNP@Fe3O4 nanoparticles as reusable carriers for reversible enzyme immobilization, Chemical Engineering Journal, 2016, 286, 272–281.
• 20. Ha, N.Y, Kim, S.H, Lee, T.G, Han, S.Y, Rapid characterization of protein chips using microwave-assisted protein tryptic digestion and MALDI mass spectrometry, Langmuir, 2011, 27, 10098–10105.