Localization of Laminin and Fibronectin in Rat Testes after Diisobutyl Phthalate Exposure: Histopathologic and Immunohistochemical Study

Year 2019, Volume: 15 Issue: 3, 293 - 300, 30.09.2019

Yücel Başımoğlu Koca

https://doi.org/10.18466/cbayarfbe.570613

Abstract

In this study the effects of Diisobutyl phthalate (DIBP) from
commonly used pytalates were histopathologically investigated in order to find out whether it is one of the
causes of increased infertility in recent years. In addition, the localization of laminin and fibronectin was demonstrated
immunohistochemically after exposure to DIBP. For this purpose, three study groups from Wistar albino rats were created: experimental group, control
group and corn oil applied group. The experimental group were administered by
gavage 3 different doses (0.25-0.5-1 ml/kg/day) of DIBP mixed with corn oil
every day for 28 days. At the end of the experiment, the testis tissue samples
from all of the experimental and control group animals were investigated and
evaluated with a light microscope after routine histological and immunohistochemical
preparation processes. Reduction in spermatogenic
cell population, apoptotic and necrotic germ cells, increase in Sertoli cells, multinucleate giant cells, thickening and
dilatation in basal lamina of seminiferous tubules, oedema and shrinkage in
interstitial connective tissue were
observed in DIBP treatment groups. The decrease
in localization of fibronectin and laminin immunoreactivity in seminiferous
tubules was observed. The
results of this study demonstrated that DIBP causes serious damage to the
testicular tissue, especially in terms of spermatogenesis. For this reason, it has come to the conclusion that the material
in question may cause infertility in male individuals.

Keywords

Diisobutyl phthalate, infertility, testes, laminin, fibronectin, histopathology

Supporting Institution

ADÜ/BAP

Project Number

FEF-2014/018

Thanks

I thank the Adnan Menderes University for support of this research through grant number FEF-2014/018. Also, I thank Merve Akyıldız, master student, for the contribution of the laboratory work.

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