Anthemis cotula'dan Fenolik ve Flavonoid Bileşiklerinin Ultrason Destekli Ekstraksiyonu: Antioksidan Potansiyel Değerlendirmesi

Year 2025, Volume: 21 Issue: 3, 50 - 55, 26.09.2025

Fatma Özlem Kargın Solmaz , Cengiz Sarıkürkcü

https://doi.org/10.18466/cbayarfbe.1627552

Abstract

Tıbbi bitkilerde etkili özütleme yöntemleri ve biyolojik etkinlik potansiyelinin araştırılması, doğal ürün araştırmalarında önemli bir odak noktası olmaya devam etmektedir. Bu çalışmanın amacı, Anthemis cotula L.'den ultrason-destekli özütleme yöntemi kullanılarak elde edilen metanol ve su özütlerinin kimyasal bileşimini ve antioksidan özelliklerini değerlendirmektir. Özütler, özüt verimi, toplam fenolik madde içeriği (TPC), toplam flavonoid içeriği (TFC) ve altı farklı analizle antioksidan aktiviteleri açısından karakterize edilmiştir: CUPRAC, FRAP, fosfomolibden, DPPH, ABTS ve demir iyonu şelatlama aktivitesi. Su özütü (%19.99), metanol özütüne (11.43%) kıyasla daha yüksek bir verim göstermiştir. Ancak, metanol özütü (56.27 mg GAEs/g ekstrakt ve 27.98 mg REs/g ekstrakt), su özütüne kıyasla daha yüksek TPC ve TFC değerlerine sahip olup, sırasıyla 64.33 mg GAEs/g özüt ve 39.22 mg REs/g özüt olarak ölçülmüştür (p < 0.05). Antioksidan analizleri, metanol özütünün daha güçlü bir indirgeme gücüne sahip olduğunu göstermiştir; CUPRAC ve FRAP değerleri sırasıyla; 262.83 ve 154.98 mg TEs/g özüt iken, su özütünün değerleri 235.98 ve 127.53 mg TEs/g özüt olarak bulunmuştur. DPPH ve ABTS radikal temizleme aktiviteleri özütler arasında benzerlik göstermiş olup, IC50 değerleri benzer etkinlikleri işaret etmiştir. Önemli olarak, su özütü, demir iyonu şelatlama aktivitesinde (12.30 mg EDTAEs/g özüt, IC50 = 1.06 mg/mL) metanol özütünü (8.55 mg EDTAEs/g özüt, IC50 = 1.53 mg/mL) geride bırakmıştır. Bu bulgular, A. cotula özütlerinin özellikle metal iyonlarını şelatlama ve indirgeme gücü sergileme potansiyelini vurgulamaktadır. Gelecekteki çalışmalar, bu aktivitelerden sorumlu biyolojik olarak aktif bileşenlerin izole edilmesine ve bu bileşenlerin farmasötik veya nütrosötik formülasyonlardaki potansiyel uygulamalarına odaklanabilir.

Keywords

Anthemis cotula , Ultrason Destekli Ekstraksiyon , Fenolik Bileşikler , Antioksidan Aktivite

Project Number

24.KARIYER.010

Ultrasound Assisted Extraction of Phenolic and Flavonoid Compounds from Anthemis cotula: Antioxidant Potential Assessment

Abstract

The exploration of effective extraction methods and bioactive potential in medicinal plants remains a critical focus for natural product research. This study aimed to evaluate the chemical composition and antioxidant properties of methanol and water extracts obtained from Anthemis cotula L. using ultrasound-assisted extraction. The extracts were characterized for extraction yield, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activities via six assays: CUPRAC, FRAP, phosphomolybdenum, DPPH, ABTS, and ferrous ion chelating activity. The water extract exhibited a higher yield (19.99%) compared to the methanol extract (11.43%). However, the methanol extract demonstrated superior TPC and TFC, with values of 64.33 mg GAEs/g extract and 39.22 mg REs/g extract, respectively, compared to 56.27 mg GAEs/g extract and 27.98 mg REs/g extract for the water extract (p < 0.05). Antioxidant assays revealed that the methanol extract possessed stronger reducing power, with CUPRAC and FRAP values of 262.83 and 154.98 mg TEs/g extract, respectively, compared to 235.98 and 127.53 mg TEs/g extract for the water extract. DPPH and ABTS radical scavenging activities were comparable between extracts, with IC50 values indicating similar efficacy. Notably, the water extract excelled in ferrous ion chelating activity (12.30 mg EDTAEs/g extract, IC50 = 1.06 mg/mL), outperforming the methanol extract (8.55 mg EDTAEs/g extract, IC50 = 1.53 mg/mL). These findings underscore the potential of A. cotula extracts, particularly in chelating metal ions and exhibiting reducing power. Future studies may focus on isolating bioactive compounds responsible for these activities and exploring their applications in pharmaceutical or nutraceutical formulations.

Keywords

Anthemis cotula , Ultrasound assisted extraction , Phenolic compounds , Antioxidant activity

Supporting Institution

Afyonkarahisar Health Sciences University Scientific Research Projects Coordination Unit

Project Number

24.KARIYER.010

Thanks

The research was funded by the Afyonkarahisar Health Sciences University Scientific Research Projects Coordination Unit under project number 24.KARIYER.010. The authors sincerely thank Dr. Olcay CEYLAN for his critical contributions in identifying the plant material used in the study

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