Influence of Citrobacter freundii Infection on Ion Levels of Model Organism Galleria mellonella Larvae

Abstract

In recent years, Galleria mellonella larvae have been used as a model of infection. In this study, the effects of Citrobacter freundii infection, which has many pathophysiological effects such as urinary system, respiratory system, bacteremia, catheter infections and meningitis in mammals, on ion balance in hemolymph of G. mellonella larvae were investigated. Calcium levels in hemolymph of G. mellonella larvae significantly decreased 8 hours after the treatment when compared to the control group. Iron levels significantly increased 2 and 4 hours after bacterial infection. However, it was statistically reduced 8 hours after the C. freundii treatment compared to the control group. In addition, potassium and magnesium levels were significantly decreased in hemolymph of G. mellonella larvae due to all C. freundii treatments. Phosphorus levels increased in hemolymph of G. mellonella larvae 8 hours after C. freundii infection, but there was no statistical difference. In the present study demonstrated that C. freundii infection disrupts the ion balance of G. mellonella larvae.

Keywords

• Infection modelIon
• Ion balance
• Citrobacter freundii
• Galleria mellonella

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