Seksüel Siklus Döngülerinin Proöstrus, Metöstrus-Diöstrus Ve Anöstrus Dönemlerinde Kangal Veya Kangal Melezi Dişi Köpeklerin Uterus Dokusunda Muc1 Ve Muc16 Ekspresyonlarının Araştırılması

Abstract

Bu çalışmada, seksüel siklus döngülerinin proöstrus, metoöstrus-diöstrus ve anöstrus dönemlerinde Kangal veya Kangal melezi dişi köpeklerin uterus dokusunda MUC1 ve MUC16 ekspresyonları immünohistokimyasal yöntemle incelendi. Çalışmada 3-6 yaş arası sağlıklı ve sahipli Kangal veya Kangal melezi 15 köpeğin uterus dokuları kullanıldı. Köpekler seksüel siklus dönemlerine göre proöstrus (n=5), metöstrus-diöstrus (n=3) ve anöstrus (n=7) olarak 3 gruba ayrıldı. Sonuç olarak, MUC1'in köpek uterusunda hem kornu hem de korpus uteri epitel hücrelerinde, kript epitel hücrelerinde, uterus bezi epitel hücrelerinde, stratum vaskülaris, miyometriyum ve perimetriyum tabakalarından eksprese edildiği tespit edildi. Östrus siklusları karşılaştırıldığında metöstrus-diöstrus döneminde ekspresyonun diğer dönemlere göre daha fazla arttığı saptandı. MUC16'nın ekspresyonu, metöstrus-diöstrus döneminde kornu uteri'de gözlendi. Bu çalışmada MUC1 ve MUC16'nın köpeklerin uterus dokusundan eksprese edildiği ve salınımlarının hormonlardan etkilendiği tespit edildi.

Keywords

• Köpek
• Östrus siklusu
• Musin
• Uterus

Investigation Of Muc1 and Muc16 Expressions in Uterus Tissue of Kangal or Kangal Cross-Bred Bitches in Proestrus, Metestrus-Diestrus and Anestrus Periods of Sexual Cycles

Abstract

Mucins are glycoprotein molecules found in mucus secretion. They have important functions in the reproductive system such as sperm motility, elimination of bacterial damage and facilitating implantation. In this study, MUC1 and MUC16 expressions in uterine tissues of Kangal or Kangal cross-bred bitchs in proestrus, metoestrus-dioestrus and anoestrus sexual periods were investigated by immunohistochemical method. Uterine tissues of 15 healthy and adopted Kangal or Kangal cross-bred bitches aged 3-6 years were used in the study. The biches were divided into 3 groups according to sexual cycle periods as proestrus (n=5), metaestrus-dioestrus (n=3) and anaestrus (n=7). As a result, MUC1 was found to be expressed in both cornu and corpus uteri epithelial cells, crypt epithelial cells, uterine gland cells, stratum vascularis, myometrium and perimetrium layers in canine uterus. When oestrus cycles were compared, it was found that expression increased more in the metoestrus-dioestrus phase compared to other phases. Expression of MUC16 was observed in the cornu uteri during the metoestrus-dioestrus phase. In this study, MUC1 and MUC16 were determined to be expressed in the uterine tissue of bitches and their release was found to be affected by hormones.

Keywords

• bitchs
• estrous cycle
• mucin
• uterus

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