Investigation of Antibacterial Effects of Laurus nobilis Extracts on Some Pathogenic Bacteria and the Interactions of Components with MreC Protein by Molecular Docking Method

Abstract

The development of multiple antibiotic resistance by pathogenic microorganisms causes prolongation of treatment periods, increased mortality rates, and economic losses. In this study, the need to develop alternative antibacterial agents due to the development of resistance to conventional antibiotics was addressed. The antibacterial effects of extracts obtained from Laurus nobilis leaves against Escherichia coli and Proteus mirabilis pathogens were investigated. In the study, it was determined that L. nobilis leaf extracts, especially at high doses (50 µL), provided inhibition on both pathogens. In addition, the interaction potential of the volatile oil components of bay leaf with proteins that control bacterial cell wall formation was investigated by molecular docking method. The results show that L. nobilis extracts may be an effective alternative, especially against pathogens that develop multiple antibiotic resistance.

Keywords

• Pathogen
• Antibacterial
• MreC
• Escherichia coli
• Laurus nobilis

Laurus nobilis L. Ekstraktlarının Bazı Patojen Bakteriler Üzerindeki Antibakteriyel Etkileri ve Bileşenlerinin MreC Proteini ile Etkileşimlerinin Moleküler Kenetlenme Yöntemi ile Değerlendirilmesi

Abstract

Patojen mikroorganizmaların çoklu antibiyotik direnci geliştirmesi tedavi sürelerinin uzaması, ölüm oranlarının artması ve ekonomik kayıplara neden olmaktadır. Bu çalışmada, geleneksel antibiyotiklere karşı gelişen direnç nedeniyle alternatif antibakteriyel ajanlar geliştirilmesi ihtiyacı ele alınmıştır. Laurus nobilis yapraklarından elde edilen ekstraktların Escherichia coli ve Proteus mirabilis patojenlerine karşı antibakteriyel etkileri araştırılmıştır. Araştırmada, L. nobilis yaprak ekstraktlarının, özellikle yüksek dozlarda (50 µL), her iki patojen üzerinde de inhibisyon sağladığı belirlenmiştir. Ayrıca, moleküler kenetlenme yöntemi ile defne yaprağının uçucu yağ bileşenlerinin bakteri hücre duvarı oluşumunu kontrol eden proteinlerle etkileşim potansiyeli incelenmiştir. Sonuçlar, L. nobilis ekstraktlarının, özellikle çoklu antibiyotik direnci geliştiren patojenlere karşı, etkili bir alternatif olabileceğini göstermektedir.

Keywords

• Patojen
• Antibakteriyel
• MreC
• Escherichia coli
• Laurus nobilis

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