Immunohistochemical Investigation of Trk-A Receptor Levels in Pancreatic Tissue of Cumin (Cuminum cyminum) Plant Essential Oil Treated-Mice

Abstract

This study was conducted to immunohistochemically investigate Trk-A receptor levels in pancreas tissue of mice treated by cumin (Cuminum cyminum) plant essential oil. Mice were grouped into control group (n = 10) and trial group (n = 10). No application was performed to the mice in the control group. The mice in trial group were treated by 500 mg/kg of oral cumin essential oil every 24 hours for two days. At the end of study, the pancreatic tissues obtained were blocked in paraffin following routine histological processes. Triple staining was performed to the sections taken from these blocks to examine general histological structure of pancreas. Acinus, islets of Langerhans, pars initialis, pars excretory and ductus excretorius were determined in mice pancreas. Immunohistochemical studies showed that all mice had Trk-A immunoreactivity in pancreatic tissue. Moderate immunoreactivity in acini and weak immunoreactivity in islets of Langerhans and excretory ducts were detected in pancreas tissue of mice in control and trial groups. It was determined that there was no difference between the groups in terms of Trk-A immunoreactivity in acini and islets of Langerhans. Based on the immunohistochemical results, cumin was used in field of diuretic, degassing, digestion facilitator, antimicrobial and antidiabetic effects in field of traditional medicine; It was concluded that Trk-A receptor synthesized from pancreatic tissue does not change its levels.

Keywords

• Cumin
• pancreas
• Trk-A

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