Akrilamidin İn-vivo Genotoksisitesi Üzerine Fenolik Bileşiklerden Pelargonidin ve Gallik Asidin Etkileri

Year 2020, Volume: 7 Issue: 1, 9 - 27, 28.06.2020

Pinar Aksu Kılıçle Abdullah Doğan

Abstract

Akrilamid genotoksik etkili olup, muhtemel karsinojenler arasında sınıflandırılmıştır. Endüstride kullanım alanı olan akrilamid, ayrıca yüksek ısıda pişirilen karbonhidratça zengin gıdalarda bulunmaktadır. Bu çalışmanın amacı, fare kemik iliği hücrelerinde akrilamidin genotoksisitesine karşı pelargonidin ve gallik asitin etkilerini ortaya çıkarmaktır. Akrilamidin genotoksik etkilerini araştırmak amacıyla kromozomal aberasyon, mikronükleus ve mitotik indeks testleri kullanıldı. Araştırmada, akrilamidin genotoksisitesine karşı pelargonidin ve gallik asitin antigenotoksik etkileri aynı yöntemlerle çalışıldı. Araştırma sonucunda Akrilamidin in vivo klastojenik etkisinin olduğu saptandı. Akrilamidin bu spesifik etkisinin doza bağlı olduğu tespit edildi. Araştırmada, Gallik asitin genotoksik ve sitotoksik etkili olmadığı belirlendi. Kromozomal aberasyon, mikronükeus ve mitotik indeks testlerinde Gallik asitin, Akrilamidin neden olduğu genotoksisite frekansında önemli derecede düşüşe neden olduğu gözlendi. Pelargonidin’in genotoksik ve sitotoksik etkileri bulunmadı. Bu araştırmada kullanılan kromozomal aberasyon, mikronükleus, ve mitotoik indeks testleri sonucuna göre, akrilamidin genotoksik ve sitotoksik, gallik asit ve pelergonidin’in ise antigenotoksik etkili olduğu, ayrıca gallik asitin pelargonidin’den daha güçlü antigenotoksik etki gösterdiği saptandı.

Supporting Institution

Kafkas Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

2010-VF-47

Thanks

*Bu çalışma Kafkas Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından Doktora Tez Projesi olarak desteklenmiştir. (Proje Numarası: 2010-VF-47).

Effects of Pelargonidine and Gallic Acid as Phenolic Compounds on In-vivo Genotoxicity of Acrylamide

Abstract

Acrylamide is genotoxic and is classified among possible carcinogens. Acrylamide, which has an area of use in the industry, is also found in carbohydrate-rich foods cooked at high temperatures. The aim of this study is to reveal the effects of pelargonidine and gallic acid against the genotoxic effect of acrylamide in mouse bone marrow cells. Chromosomal aberration, micronucleus and mitotic index tests were used to investigate the genotoxic effects of acrylamide. In addition, the antigenotoxic effect of pelargonidine and gallic acid against the genotoxicity of acrylamide was investigated by same methods. As a result of the research, it was determined that acrylamide had a clastogenic effect in vivo. These acrylamide-specific effects were dose-dependent. In this study, it was determined that gallic acid was not genotoxic and cytotoxic. In chromosomal aberration, micronucleus and mitotic index tests, gallic acid caused a significant decrease in the frequency of genotoxicity caused by acrylamide. The genotoxic and cytotoxic effects of pelargonidine were not found. According to the results of chromosomal aberration, micronucleus, and mitotoic index tests used in this study, acrylamide was found to have a genotoxic and cytotoxic effect, gallic acid and pelergonidine had an antigenotoxic effect, and gallic acid had a stronger antigenotoxic effect than pelargonidine.

Keywords

Acrylamide, Gallic acid, Pelargonidine, Chromosomal aberration, Micronucleus

Project Number

2010-VF-47

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