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Influence of Citrobacter freundii Infection on Ion Levels of Model Organism Galleria mellonella Larvae

Year 2021, Volume: 8 Issue: 2, 139 - 147, 31.12.2021
https://doi.org/10.48138/cjo.1006143

Abstract

In recent years, Galleria mellonella larvae have been used as a model of infection. In this study, the effects of Citrobacter freundii infection, which has many pathophysiological effects such as urinary system, respiratory system, bacteremia, catheter infections and meningitis in mammals, on ion balance in hemolymph of G. mellonella larvae were investigated. Calcium levels in hemolymph of G. mellonella larvae significantly decreased 8 hours after the treatment when compared to the control group. Iron levels significantly increased 2 and 4 hours after bacterial infection. However, it was statistically reduced 8 hours after the C. freundii treatment compared to the control group. In addition, potassium and magnesium levels were significantly decreased in hemolymph of G. mellonella larvae due to all C. freundii treatments. Phosphorus levels increased in hemolymph of G. mellonella larvae 8 hours after C. freundii infection, but there was no statistical difference. In the present study demonstrated that C. freundii infection disrupts the ion balance of G. mellonella larvae.

References

  • Aci, R., Muderrisoğlu, S., Duran, U., Shakouri, N., Çiftci, G. (2020). Content of minerals in muscle tissue of diploid rainbow trout (Oncorhynchus mykiss) in freshwater (Derbent Dam) and seawater (The Black Sea). Journal of Elementology, 25(4), 1375-1384. https://doi.org/10.5601/jelem.2020.25.2.2017
  • Ames, L., Duxbury, S., Pawlowska, B., Ho, H.L., Haynes, K., Bates, S. (2017). Galleria mellonella as a host model to study Candida glabrata virulence and antifungal efficacy. Virulence, 8 (8), 1909-1917. https://doi.org/10.1080/21505594.2017.1347744
  • Anderson, M.T., Mitchell, L.A., Zhao, L., Mobley, H.L. (2018). Citrobacter freundii fitness during bloodstream infection. Scientific Reports, 8 (1), 1-14. https://doi.org/10.1038/s41598-018-30196-0
  • Asai, M., Li, Y., Spiropoulos, J., Cooley, W., Everest, D., Robertson, B.D., Langford, P.R., Newton, S.M. (2020). A novel biosafety level 2 compliant tuberculosis infection model using a Δ leuD Δ panCD double auxotroph of Mycobacterium tuberculosis H37Rv and Galleria mellonella. Virulence, 11 (1), 811-824. https://doi.org/10.1080/21505594.2020.1781486.
  • Bronskill, J. (1961). A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pralidae). Journal of the Lepidopterists' Society, 15 (2), 102–104
  • Büyükgüzel, E., Büyükgüzel, K. (2021). Oxidative Impact of Dietary Triclabendazole in Galleria mellonella. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27 (3), 301-306, 2021. https://doi.org/10.9775/kvfd.2020.25170
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. (2010). Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology - Part A, 156 (2), 176–183. https://doi.org/10.1016/j.cbpa.2010.01.020
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. (2010). Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology Part A, 156 (2), 176–183. https://doi.org/10.1016/j.cbpa.2010.01.020
  • Chen, X.R., Hu, Q.B., Yu, X.Q., Ren, S.X. (2014). Effects of destruxins on free calcium and hydrogen ions in insect hemocytes. Insect Science, 21 (1), 31-38. https://doi.org/10.1111/1744-7917.12028
  • Clark, E.W. (1958). A review of literature on calcium and magnesium in insects. Annals of the Entomological Society of America, 51(2), 142-154. https://doi.org/10.1111/1744-7917.12028.
  • Dunham, R.A. (2004). Aqua culture and fisheries biotechnology: Genetic approaches. Book review, p. 145.
  • Gaete, H., Álvarez, M., Lobos, G., Soto, E., Jara-Gutiérrez, C. (2017). Assessment of oxidative stress and bioaccumulation of the metals Cu, Fe, Zn, Pb, Cd in the polychaete Perinereis gualpensis from estuaries of central Chile. Ecotoxicology and Environmental Safety, 145, 653-658. https://doi.org/10.1016/j.ecoenv.2017.07.073
  • Harding, C.R., Schroeder, G.N., Reynolds, S., Kosta, A., Collins, J.W., Mousnier, A., Frankel, G. (2012). Legionella pneumophila pathogenesis in the Galleria mellonella infection model. Infection and Immunity, 80 (8), 2780-2790. https://doi.org/10.1128/IAI.00510-12
  • Hyršl, P., Büyükgüzel, E., Büyükgüzel, K. (2007). The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella. Archives of Insect Biochemistry and Physiology, 66 (1), 23–31. https://doi.org/10.1002/arch.20194
  • Kastamonuluoğlu, S., Büyükgüzel, K., Büyükgüzel, E. (2020). The use of dietary antifungal agent terbinafine in artificial diet and its effects on some biological and biochemical parameters of the model organism Galleria mellonella (Lepidoptera: Pyralidae). Journal of Economic Entomology, 113(3),1110–1117. https://doi.org/10.1093/jee/toaa039
  • Kayış, T., Coşkun, M., Dursun, O., Emre, I. (2015). Alterations in antioxidant enzyme activity, lipid peroxidation, and ion balance induced by dichlorvos in Galleria mellonella (Lepidoptera: Pyralidae). Annals of the Entomological Society of America, 108(4),570–574. https://doi.org/10.1093/aesa/sav038
  • Khorasani, G., Salehifar, E., Eslami, G. (2008). Profile of microorganisms and antimicrobial resistance at a tertiary care referral burn centre in Iran: emergence of Citrobacter freundii as a common microorganism. Burns, 34 (7), 947-952. https://doi.org/10.1016/j.burns.2007.12.008
  • Kwadha, C.A., Ongamo, G.O., Ndegwa, P.N., Raina, S.K., Fombong, A.T. (2017). The biology and control of the greater wax moth, Galleria mellonella. Insects 8(2),61. https://doi.org/10.3390/insects8020061
  • Liu, L.H., Wang, N.Y., Wu, A.Y.J., Lin, C.C., Lee, C.M., Liu, C.P. (2018). Citrobacter freundii bacteremia: Risk factors of mortality and prevalence of resistance genes. Journal of Microbiology, Immunology and Infection, 51(4), 565-572. https://doi.org/10.1016/j.jmii.2016.08.016
  • Marshall, W.S., Grosell, M. (2006). Ion transport, osmoregulation, and acid-base balance. In: The Physiology of Fishes. Evans D.H., Claiborne J.B., eds. CRC Press, Boca Raton, 177- 230.
  • Moore, G.M., Gitai, Z. (2020). Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model. PloS one, 15 (3), e0230006. https://doi.org/10.1371/JOURNAL.PONE.0230006
  • Ochoa, S., Fernández, F., Devotto, L., France Iglesias, A., Collado, L. (2021). Virulence assessment of enterohepatic Helicobacter species carried by dogs using the wax moth larvae Galleria mellonella as infection model. Helicobacter, e12808. https://doi.org/10.1111/hel.12808.
  • Roche, M., Rondeau, P., Singh, N.R., Tarnus, E., Bourdon, E. (2008). The antioxidant properties of serum albumin. FEBS Letters, 582(13), 1783-1787. https://doi.org/10.1016/j.febslet.2008.04.057
  • Southall, T.D., Terhzaz, S., Cabrero, P., Chintapallii, V.R., Evans, J.M., Dow J.A.T., Davies, S.A. (2006). Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases. Physiological Genomics, 26 (1), 35-45. https://doi.org/10.1152/physiolgenomics.00038.2006
  • Sowers, A.D., Young, S.P., Grosell, M., Browdy, C.L., Tomasso, J.R. (2006). Hemolymph osmolality and cation concentrations in Litopenaeus vannamei during exposure to artificial sea salt or a mixed-ion solution: relationship to potassium flux. Comparative Biochemistry and Physiology Part A, 145(2), 176-180. https://doi.org/10.1016/j.cbpa.2006.06.008
  • Sugeçti, S. (2021). Pathophysiological effects of Klebsiella pneumoniae infection on Galleria mellonella as an invertebrate model organism. Archives of Microbiology, 203, 3509–3517 https://doi.org/10.1007/s00203-021-02346-y
  • Sugeçti, S., Büyükgüzel, K. (2018). Effects of oxfendazole on metabolic enzymes in hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) larvae reared on artificial diet. Karaelmas Fen ve Mühendislik Dergisi, 8(2),590–594. https://doi.org/10.7212/Fzkufbd.v8i2.1380
  • Sugeçti, S., Büyükgüzel, K., Büyükgüzel, E. (2016). Laboratory assays of the effects of oxfendazole on biological parameters of Galleria mellonella (Lepidoptera: Pyralidae). Journal of Entomological Science, 51(2),129–137. https://doi.org/10.18474/JES15-36.1
  • Tunçsoy, B., Sugeçti, S., Büyükgüzel, E., Özalp, P., Büyükgüzel, K. (2021). Effects of Copper Oxide Nanoparticles on Immune and Metabolic Parameters of Galleria mellonella L. Bulletin of Environmental Contamination and Toxicology, 107: 412–420. https://doi.org/10.1007/s00128-021-03261-0
Year 2021, Volume: 8 Issue: 2, 139 - 147, 31.12.2021
https://doi.org/10.48138/cjo.1006143

Abstract

References

  • Aci, R., Muderrisoğlu, S., Duran, U., Shakouri, N., Çiftci, G. (2020). Content of minerals in muscle tissue of diploid rainbow trout (Oncorhynchus mykiss) in freshwater (Derbent Dam) and seawater (The Black Sea). Journal of Elementology, 25(4), 1375-1384. https://doi.org/10.5601/jelem.2020.25.2.2017
  • Ames, L., Duxbury, S., Pawlowska, B., Ho, H.L., Haynes, K., Bates, S. (2017). Galleria mellonella as a host model to study Candida glabrata virulence and antifungal efficacy. Virulence, 8 (8), 1909-1917. https://doi.org/10.1080/21505594.2017.1347744
  • Anderson, M.T., Mitchell, L.A., Zhao, L., Mobley, H.L. (2018). Citrobacter freundii fitness during bloodstream infection. Scientific Reports, 8 (1), 1-14. https://doi.org/10.1038/s41598-018-30196-0
  • Asai, M., Li, Y., Spiropoulos, J., Cooley, W., Everest, D., Robertson, B.D., Langford, P.R., Newton, S.M. (2020). A novel biosafety level 2 compliant tuberculosis infection model using a Δ leuD Δ panCD double auxotroph of Mycobacterium tuberculosis H37Rv and Galleria mellonella. Virulence, 11 (1), 811-824. https://doi.org/10.1080/21505594.2020.1781486.
  • Bronskill, J. (1961). A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pralidae). Journal of the Lepidopterists' Society, 15 (2), 102–104
  • Büyükgüzel, E., Büyükgüzel, K. (2021). Oxidative Impact of Dietary Triclabendazole in Galleria mellonella. Kafkas Universitesi Veteriner Fakultesi Dergisi, 27 (3), 301-306, 2021. https://doi.org/10.9775/kvfd.2020.25170
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. (2010). Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology - Part A, 156 (2), 176–183. https://doi.org/10.1016/j.cbpa.2010.01.020
  • Büyükgüzel, E., Hyršl, P., Büyükgüzel, K. (2010). Eicosanoids mediate hemolymph oxidative and antioxidative response in larvae of Galleria mellonella L. Comparative Biochemistry and Physiology Part A, 156 (2), 176–183. https://doi.org/10.1016/j.cbpa.2010.01.020
  • Chen, X.R., Hu, Q.B., Yu, X.Q., Ren, S.X. (2014). Effects of destruxins on free calcium and hydrogen ions in insect hemocytes. Insect Science, 21 (1), 31-38. https://doi.org/10.1111/1744-7917.12028
  • Clark, E.W. (1958). A review of literature on calcium and magnesium in insects. Annals of the Entomological Society of America, 51(2), 142-154. https://doi.org/10.1111/1744-7917.12028.
  • Dunham, R.A. (2004). Aqua culture and fisheries biotechnology: Genetic approaches. Book review, p. 145.
  • Gaete, H., Álvarez, M., Lobos, G., Soto, E., Jara-Gutiérrez, C. (2017). Assessment of oxidative stress and bioaccumulation of the metals Cu, Fe, Zn, Pb, Cd in the polychaete Perinereis gualpensis from estuaries of central Chile. Ecotoxicology and Environmental Safety, 145, 653-658. https://doi.org/10.1016/j.ecoenv.2017.07.073
  • Harding, C.R., Schroeder, G.N., Reynolds, S., Kosta, A., Collins, J.W., Mousnier, A., Frankel, G. (2012). Legionella pneumophila pathogenesis in the Galleria mellonella infection model. Infection and Immunity, 80 (8), 2780-2790. https://doi.org/10.1128/IAI.00510-12
  • Hyršl, P., Büyükgüzel, E., Büyükgüzel, K. (2007). The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella. Archives of Insect Biochemistry and Physiology, 66 (1), 23–31. https://doi.org/10.1002/arch.20194
  • Kastamonuluoğlu, S., Büyükgüzel, K., Büyükgüzel, E. (2020). The use of dietary antifungal agent terbinafine in artificial diet and its effects on some biological and biochemical parameters of the model organism Galleria mellonella (Lepidoptera: Pyralidae). Journal of Economic Entomology, 113(3),1110–1117. https://doi.org/10.1093/jee/toaa039
  • Kayış, T., Coşkun, M., Dursun, O., Emre, I. (2015). Alterations in antioxidant enzyme activity, lipid peroxidation, and ion balance induced by dichlorvos in Galleria mellonella (Lepidoptera: Pyralidae). Annals of the Entomological Society of America, 108(4),570–574. https://doi.org/10.1093/aesa/sav038
  • Khorasani, G., Salehifar, E., Eslami, G. (2008). Profile of microorganisms and antimicrobial resistance at a tertiary care referral burn centre in Iran: emergence of Citrobacter freundii as a common microorganism. Burns, 34 (7), 947-952. https://doi.org/10.1016/j.burns.2007.12.008
  • Kwadha, C.A., Ongamo, G.O., Ndegwa, P.N., Raina, S.K., Fombong, A.T. (2017). The biology and control of the greater wax moth, Galleria mellonella. Insects 8(2),61. https://doi.org/10.3390/insects8020061
  • Liu, L.H., Wang, N.Y., Wu, A.Y.J., Lin, C.C., Lee, C.M., Liu, C.P. (2018). Citrobacter freundii bacteremia: Risk factors of mortality and prevalence of resistance genes. Journal of Microbiology, Immunology and Infection, 51(4), 565-572. https://doi.org/10.1016/j.jmii.2016.08.016
  • Marshall, W.S., Grosell, M. (2006). Ion transport, osmoregulation, and acid-base balance. In: The Physiology of Fishes. Evans D.H., Claiborne J.B., eds. CRC Press, Boca Raton, 177- 230.
  • Moore, G.M., Gitai, Z. (2020). Both clinical and environmental Caulobacter species are virulent in the Galleria mellonella infection model. PloS one, 15 (3), e0230006. https://doi.org/10.1371/JOURNAL.PONE.0230006
  • Ochoa, S., Fernández, F., Devotto, L., France Iglesias, A., Collado, L. (2021). Virulence assessment of enterohepatic Helicobacter species carried by dogs using the wax moth larvae Galleria mellonella as infection model. Helicobacter, e12808. https://doi.org/10.1111/hel.12808.
  • Roche, M., Rondeau, P., Singh, N.R., Tarnus, E., Bourdon, E. (2008). The antioxidant properties of serum albumin. FEBS Letters, 582(13), 1783-1787. https://doi.org/10.1016/j.febslet.2008.04.057
  • Southall, T.D., Terhzaz, S., Cabrero, P., Chintapallii, V.R., Evans, J.M., Dow J.A.T., Davies, S.A. (2006). Novel subcellular locations and functions for secretory pathway Ca2+/Mn2+-ATPases. Physiological Genomics, 26 (1), 35-45. https://doi.org/10.1152/physiolgenomics.00038.2006
  • Sowers, A.D., Young, S.P., Grosell, M., Browdy, C.L., Tomasso, J.R. (2006). Hemolymph osmolality and cation concentrations in Litopenaeus vannamei during exposure to artificial sea salt or a mixed-ion solution: relationship to potassium flux. Comparative Biochemistry and Physiology Part A, 145(2), 176-180. https://doi.org/10.1016/j.cbpa.2006.06.008
  • Sugeçti, S. (2021). Pathophysiological effects of Klebsiella pneumoniae infection on Galleria mellonella as an invertebrate model organism. Archives of Microbiology, 203, 3509–3517 https://doi.org/10.1007/s00203-021-02346-y
  • Sugeçti, S., Büyükgüzel, K. (2018). Effects of oxfendazole on metabolic enzymes in hemolymph of Galleria mellonella L. (Lepidoptera: Pyralidae) larvae reared on artificial diet. Karaelmas Fen ve Mühendislik Dergisi, 8(2),590–594. https://doi.org/10.7212/Fzkufbd.v8i2.1380
  • Sugeçti, S., Büyükgüzel, K., Büyükgüzel, E. (2016). Laboratory assays of the effects of oxfendazole on biological parameters of Galleria mellonella (Lepidoptera: Pyralidae). Journal of Entomological Science, 51(2),129–137. https://doi.org/10.18474/JES15-36.1
  • Tunçsoy, B., Sugeçti, S., Büyükgüzel, E., Özalp, P., Büyükgüzel, K. (2021). Effects of Copper Oxide Nanoparticles on Immune and Metabolic Parameters of Galleria mellonella L. Bulletin of Environmental Contamination and Toxicology, 107: 412–420. https://doi.org/10.1007/s00128-021-03261-0
There are 29 citations in total.

Details

Primary Language English
Subjects Environmental Sciences
Journal Section Caucasian Journal of Science
Authors

Serkan Sugeçti 0000-0003-3412-2367

Publication Date December 31, 2021
Submission Date October 7, 2021
Acceptance Date December 17, 2021
Published in Issue Year 2021 Volume: 8 Issue: 2

Cite

APA Sugeçti, S. (2021). Influence of Citrobacter freundii Infection on Ion Levels of Model Organism Galleria mellonella Larvae. Caucasian Journal of Science, 8(2), 139-147. https://doi.org/10.48138/cjo.1006143

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