Proteomic Analysis in Nifedipine Induced Gingival Overgrowth: A Pilot Study

Year 2022, , 1013 - 1024, 30.12.2022

Ece Yetiş Ayşen Yarat , Onur Eroğlu Hafize Öztürk Özener , Leyla Kuru

https://doi.org/10.33808/clinexphealthsci.1050418

Abstract

Objective: The aims of the present study were to investigate the proteomic profile of nifedipine induced overgrown gingiva and compare with non-overgrown gingival tissues obtained from the same patients. Methods: Seven subjects under nifedipine medication for at least 6 months and diagnosed as nifedipine induced gingival overgrowth (NIGO) participated in the study. Periodontal clinical parameters were recorded. Gingival tissue samples were harvested from overgrown (GO+ Group, n=7) and non-overgrown regions (GO- Group, n=7) of the same patients. Proteomics was performed using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) technique. The identified proteins were further classified according to their molecular functions, biological processes and cellular component distribution for functional gene ontology analysis using a web-based bioinformatics tool. Mann Whitney-U and ANOVA tests were performed to compare clinical parameters and identified proteins with proteomics, respectively. Results: Bleeding on probing and gingival overgrowth index of the GO+ group were statistically significantly higher than the GO- group (p<0.05, p<0.01, respectively). A total of 143 proteins were identified in 14 gingival tissue samples using proteomics. Among the proteins identified, 79 of them were detected in higher quantities in the GO+ group (p<0.05) whereas remaining 64 were found higher in the GO- group (p<0.05). The analysis of functional gene ontology demonstrated that certain proteins exhibit roles in either stimulatory or inhibitory processes including cell proliferation, growth and apoptosis. Conclusion: The proteomic profiles of overgrown and non-overgrown gingiva suggest that the identified proteins expressed at different levels in both groups may contribute to the pathogenesis and progression of NIGO.

Keywords

gingival overgrowth, nifedipine, proteomics

Project Number

SAG-C-DRP-110718-0437

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