Evaluation of in vitro Antioxidant, Antimicrobial and Cytotoxic Activities of Crude Ethanol Extract and Fractions of Achillea sintenisii Hub. Mor.

Year 2023, , 517 - 524, 11.09.2023

Nuraniye Eruygur , Mehmet Ataş , Mehmet Tekin , Ozge Cevik

https://doi.org/10.33808/clinexphealthsci.1058614

Abstract

Objective: The Achillea species have been used to treat various ailments due to its anti-inflammatory, hemostatic, spasmolytic and cholagogue effects in the Turkish traditional medicine. However, there is no biological activity studies on some Achillea species except for the well-knowns. This work aimed to determine the antioxidant, antimicrobial and cytotoxic activities of the crude ethanolic extracts and fractions of Achillea sintenisii using in vitro methods.
Methods: The antioxidant activity was investigated by DPPH (1,1´-diphenyl- 2-picrylhydrazyl), ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging, total phenol and total flavonoid content, and iron chelating methods. Antimicrobial activity evaluated by micro-plate dilution method against five test organisms. Cytotoxicity was determined by MTT method using MCF-7 breast cancer cell line and PC-3 prostate cancer cell line. Apoptosis was also measured by AO/EB staining.
Results: The n-Hexane fractions showed the highest antimicrobial and cytotoxic activities, respectively. Administration of the extracts on the cancer cells showed a concentration dependent inhibition on cell proliferation. The anti-proliferation effect could be via apoptosis and associated with the cell death.
Conclusion: The results showed that the extracts demonstrated antioxidant, antimicrobial, and cytotoxic activity, also supports the claims of traditional usage.

Keywords

Achillea sintenisii, antioxidant;, microdilution, cytotoxicity

Supporting Institution

Sivas Cumhuriyet University

Project Number

CÜBAP ECZ-020

Thanks

This work was supported by the Cumhuriyet University Academic Research Grants [grant number: CÜBAP ECZ-020].

References

• Baser KHC, Demirci B, Kaiser R, Duman H. Composition of the essential oil of Achillea phrygia Boiss. et Ball. J Essent Oil Res. 2000;12(3):327-329.
• Baytop T. Therapy with medicinal plants in Turkey past and present. 2 nd. Istanbul: Nobel Tıp Kitabevi; 1999.
• Mohammadhosseini M, Sarker SD, Akbarzadeh A. Chemical composition of the essential oils and extracts of Achillea species and their biological activities: A review. J Ethnopharmacol 2017;199:257-315.
• Ertaş A, Boǧa M, Haşimi N, Yeşil Y, Gören AC, Topçu G, Kolak U. Antioxidant, anticholinesterase, and antimicrobial activities and fatty acid constituents of Achillea cappadocica Hausskn. et Bornm. Turkish J Chem 2014; 38(4): 592-599.
• Polatoǧlu K, Karakoç ÖC, Gören N. Phytotoxic, DPPH scavenging, insecticidal activities and essential oil composition of Achillea vermicularis, A. teretifolia and proposed chemotypes of A. biebersteinii (Asteraceae). Ind Crops Prod 2013;51:35-45.
• Majnooni MB, Mohammadi-Farani A, Gholivand MB, Nikbakht MR, Bahrami GR. Chemical composition and anxiolytic evaluation of Achillea Wilhelmsii C. Koch essential oil in rat. Res Pharm Sci 2013;8(4):269-275.
• Dokhani S, Cottrell T, Khajeddin J, Mazza G. Analysis of aroma and phenolic components of selected Achillea species. Plant Foods Hum Nutr 2005;60(2):55-62.
• Gören N, Öksüz S, AU. a Sesquiterpene lactone, sintenin, from Achillea sintenisii. Phytochemistry. 1988;27(1979):3670-3672.
• Küpeli E, Orhan İ, Küsmenoglu Ş, Yeşilada E. Evaluation of anti-inflammatory and antinocicptive activity of five Anatolian Achillea species. Turk J Pharm Sci. 2007;4(2):89-99.
• Georgieva L, Gadjalova A, Mihaylova D, Pavlov A. Achillea millefolium L. - Phytochemical profile and in vitro antioxidant activity. Int Food Res J 2015;22(4):1347-1352.
• Mottaghipisheh J, Hazeri N, Valizadeh J, Maghsoodlou MT, Arjomandi R. Constituents of the essential oil and antioxidant activity of extracts of Achillea eriophora from Iran. J Essent Oil-Bearing Plants 2015;18(1):52-56.
• Baser KHC, Demirci B, Duman H. Composition of the essential oils of two endemic species from Turkey: Achillea lycaonica and A. ketenoglui. Chem Nat Compd 2001;37(3):245-252.
• García-Risco MR, Mouhid L, Salas-Pérez L, López-Padilla A, Santoyo S, Jaime L, Molina AR, Reglero G, Fornari T. Biological Activities of Asteraceae (Achillea millefolium and Calendula officinalis) and Lamiaceae (Melissa officinalis and Origanum majorana) Plant Extracts. Plant Foods Hum Nutr 2017;72(1):96-102.
• Turkoglu I, Turkoglu S, Celik S, Kahyaoglu M. Antioxidant and antimicrobial activities of Turkish endemic Achillea species. African J Microbiol Res. 2010;4(19):2034-2042.
• Bali EB, Acik L, Elci P, Sarper M, Avcu F, Vural M. In vitro anti-oxidant, cytotoxic and pro-apoptotic effects of Achillea teretifolia Willd extracts on human prostate cancer cell lines. Pharmacognosy magazine. 2015; 11: S308-15.
• Alfatemi SMH, Sharifi-Rad JS, Rad MS, Mohsenzadeh S, da Silva JAT. Chemical composition, antioxidant activity and in vitro antibacterial activity of Achillea wilhelmsii C. Koch essential oil on methicillin-susceptible and methicillin-resistant Staphylococcus aureus spp. 3 Biotech. 2015;5(1):39-44.
• Barış D, Kızıl M, Aytekin Ç, Kızıl G, Yavuz M, Çeken B, Ertekin AS. In Vitro Antimicrobial and Antioxidant Activity of Ethanol Extract of Three Hypericum and Three Achillea Species From Turkey. Int J Food Prop 2011;14(2):339-355.
• Demirci F, Demirci B, Gürbüz I, Yeşilada E, Başer KHC. Characterization and Biological Activity of Achillea teretifolia Willd. and A. nobilis L. subsp. neilreichii (Kerner) Formanek Essential Oils. Turkish J Biol. 2009;33(2):129-36.
• Karaalp C, Yurtman AN, Yavasoglu NUK. Evaluation of antimicrobial properties of Achillea L. flower head extracts. Pharm Bio 2009;47(1):86-91.
• Azaz AD, Arabaci T, Sangun MK, Yildiz B. Composition and the in vitro antimicrobial activities of the essential oils of Achillea wilhelmsii C. Koch. and Achillea lycaonica Boiss & Heldr. Asian J Chem 2008;20(2):1238-1244.
• Baser KHC, Demirci B, Demirci F, Kocak S, Akinci C, Malyer H, Güleryüz G. Composition an Antimicrobial Activity of the Essential Oil of Achillea multifida. Lett . Planta Med. 2002;68:941-943.
• Ünlü M, Daferera D, Dönmez E, Polissiou M, Tepe B, Sökmen A. Compositions and the in vitro antimicrobial activities of the essential oils of Achillea setacea and Achillea teretifolia (Compositae). J Ethnopharmacol 2002;83(1–2):117-121.
• Küpeli E, Orhan I, Küsmenoǧlu Ş̧, Yeşilada E. Evaluation of anti-inflammatory and antinociceptive activity of five Anatolian Achillea species. Turkish J Pharm Sci 2007;4(2):89-99.
• Karabay-Yavasoglu NU, Karamenderes C, Baykan S, Apaydin S. Antinociceptive and anti-inflammatory activities and acute toxicity of Achillea nobilis . subsp. neilreichii . extract in mice and rats. Pharm Biol 2007;45(2):162-168.
• Akkol EK, Koca U, Pesin I, Yilmazer D. Evaluation of the wound healing potential of Achillea biebersteinii Afan. (Asteraceae) by in vivo excision and incision models. Evidence-based Complement Altern Med 2011;2011:1-7.
• Agar OT, Dikmen M, Ozturk N, Yilmaz MA, Temel H, Turkmenoglu FP. Comparative studies on phenolic composition, antioxidant, wound healing and cytotoxic activities of selected Achillea L. species growing in Turkey. Molecules 2015;20(10):17976-18000.
• Turkoglu I, Turkoglu S, Celik S, Kahyaoglu M. Antioxidant and antimicrobial activities of Turkish endemic Achillea species. Afr J Microbiol Res 2010;4(19):2034-2042.
• Boga M, Ertas A, Eroglu-Ozkan E, Kizil M, Ceken B, Topcu G. Phytochemical analysis, antioxidant, antimicrobial, anticholinesterase and DNA protective effects of Hypericum capitatum var. capitatum extracts. South African J Bot 2016;104:249-257.
• Prakash O, Kumar A, Kumar P, Ajeet A. Anticancer Potential of Plants and Natural Products: A Review. Am J Pharmacol Sci 2013;1(6):104-115.
• Khalighi-Sigaroodi F, Ahvazi M, Yazdani D, Kashefi M. Cytotoxicity and antioxidant activity of five plant species of solanaceae family from Iran. J Med Plants 2012;11(43):41-53.
• Sarkhail P, Sahranavard S, Nikan M, Gafari S, Eslami-Tehrani B. Evaluation of the cytotoxic activity of extracts from six species of Phlomis genus. J Appl Pharm Sci 2017;7(2):180-184.
• Shirazi MT, Gholami H, Kavoosi G, Rowshan V, Tafsiry A. Chemical composition, antioxidant, antimicrobial and cytotoxic activities of Tagetes minuta and Ocimum basilicum essential oils. Food Sci Nutr 2014;2(2):146-155.
• Rahman MM, Hossain MA, Siddique SA, Biplab KP, Uddin MH. Antihyperglycemic, antioxidant, and cytotoxic activities of Alocasia macrorrhizos (L.) rhizome extract. Turkish J Biol 2012;36(5):574-579.
• Xue J, Sun Y, Wei Q, Wang C, Yang B, Kuang H, Wang QH. Chemical composition and cytotoxicity of the essential oil from different parts of Datura metel L . PubMed Commons. Nat Prod Res 2016;6419(1):26418519.
• Sökmen A, Vardar-Ünlü G, Polissiou M, Daferera D, Sökmen M, Dönmez E. Antimicrobial Activity of Essential Oil and Methanol Extracts of Achillea sintenisii Hub. Mor. (Asteraceae). Phyther Res 2003;17(9):1005-1010.
• Evans WC. Alkaloids. Trease and Evans’ Pharmacognosy. 2009. p. 353.
• Roberta Re, Nicoletta Pellegrini, Anna Proteggente, Ananth Pannala, Min Yang CR-E. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–7.
• Salama ZA, El Baz FK, Gaafar AA, Zaki MF. Antioxidant activities of phenolics, flavonoids and vitamin C in two cultivars of fennel (Foeniculum vulgare Mill.) in responses to organic and bio-organic fertilizers. J Saudi Soc Agric Sci 2015;14(1):91-99.
• Blois MS. Antioxidant determination by the use of a stable free radical. Nature 1958;181:1199-200.
• Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life sciences 2003;73(2):167-179.
• Eruygur N, Yılmaz G, Üstün O. Analgesic and antioxidant activity of some Echium species wild growing in Turkey. FABAD pharm Sci 2012;37(3):151-159.
• Oyaizu M. Studies on products of browning reaction. Antioxidative activities of products of browning reaction prepared from glucosamine. Japanese J Nutr Diet 1986;44(17):307-315.
• Eruygur N, Ataş M, Çevir Ö, Tekin M. Investigating of Phytochemicals, Antioxidant, Antimicrobial and Proliferative Properties of Different Extracts of Thymus spathulifolius Hausskn. and Velen. Endemic Medicinal Plant from Sivas, Turkey. Int J Second Metab 2017;4(1):15-166.
• Arvelo F, Sojo F, Calder I. Cytotoxic, antioxidant and antimicrobial properties of red sweet pepper (Capsicum annuum L. var. Llanerón) extracts: In vitro study. Int J Food Stud 2017;6(2):222–31.
• Şabanoğlu S, Khazneh E, Saltan G. Secondary Metabolites of Achillea sintenisii Hub.Mor. Fabad J Pharm Sci. 2017;42(3):191-197.
• Vardar-Ünlü G, Candan F, Sókmen A, Daferera D, Polissiou M, Sökmen M, Dönmez E, Tepe B. Antimicrobial and antioxidant activity of the essential oil and methanol extracts of Thymus pectinatus Fisch. et Mey. Var. pectinatus (Lamiaceae). J Agric Food Chem 2003;51(1):63-67.
• Karaalp C, Yurtman AN, Karabay Yavasoglu NU. Evaluation of antimicrobial properties of Achillea L. flower head extracts. Pharm Biol 2009;47(1):86-91.
• Boutennoun H, Boussouf L, Rawashdeh A, Al-Qaoud K, Abdelhafez S, Kebieche M, Modani K. In vitro cytotoxic and antioxidant activities of phenolic components of Algerian Achillea odorata leaves. Arab J Chem 2017;10(3):403-409.
• Yilmaz MA, Ertas A, Yener I, Akdeniz M, Cakir O, Altun M, Demirtas I, Boga M, Temel H. A comprehensive LC–MS/MS method validation for the quantitative investigation of 37 fingerprint phytochemicals in Achillea species: A detailed examination of A. coarctata and A. monocephala. J Pharm Biomed Anal 2018;154:413-424.