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Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats

Year 2023, , 308 - 315, 15.06.2023

Abdulsamet Tanık , Arzum Güler Dogru , Mehmet Gül

https://doi.org/10.33808/clinexphealthsci.1080508

Abstract

Objective: In the present paper, it was purposed to examine the late impacts of bone tissue recoveries in combination with synthetic grafts and Ankaferd Blood Stopper (ABS), which are effective in bone wound healing in diabetic rats.
Methods: A total of 64 Wistar albino male rats with diabetes were studied. A bone deformity was generated in the calvarium of diabetic rats. These diabetic rats are divided into 4 different groups. Only saline was applied to the bone defect in group 1, Beta-tricalcium phosphate (β-TCP) graft was administered to Group 2, ABS solution to Group 3, (β-TCP+ABS) were administered to group 4. Eight rats from each group were sacrificed on the 28th day and another 8 rats on the 56th day. Immunohistochemical, histopathological, and Dual Energy X-ray Absorptiometry (DEXA) analyzes of the obtained samples were made.
Results: In histopathological measurements, osteoblastic activity and bone regeneration were considerably higher in the group treated with group 2 and group 4 when compared to the control group on the 28th and 56th day (p˂ .05). Western blotting findings showed that the osteopontin (OP) and osteonectin (ON) expression at 28th day increased dramatically in the treated with group 4. DEXA analyzes revealed that BMC values in groups treated with group 2, group 3 and group 4 on 28th day were considerably higher than the control group (p˂ .05).
Conclusion: We can believe and conclude that ABS in combination with a β-TCP bone graft will produce more successful outcomes on wound
healing and formation of new bone in diabetic rats.

Keywords

Ankaferd blood stopper bone regeneration beta-tricalcium phosphate graft DEXA

Supporting Institution

Dicle Üniversitesi BAP birimi

Project Number

DİŞ.15.008

Thanks

In this study, histological sections were presented to Prof Dr. Engin Deveci and radiological analysis by Prof Dr. Veysi Akpolat. I sincerely thank them for their valuable technical contribution.

References

  • Detert J, Pischon N, Burmester GR, Buttgereit F. The association between rheumatoid arthritis and periodontal disease. Arthritis Res Ther. 2010;12(5):218. DOI: 10.1186/ar3106
  • Bascones-Martinez A, Matesanz-Perez P, Escribano-Bermejo M, González-Moles MÁ, Bascones-Ilundain J, Meurman JH. Periodontal disease and diabetes-review of the literature. Med Oral Patol Oral Cir Bucal. 2011;16(6):722-729. DOI:10.4317/medoral.17032
  • Semyari H, Rajipour M, Sabetkish S, Sabetkish N, Mashhadi AF, Kajbafzadeh AM. Evaluating the bone regeneration in calvarial defect using osteoblasts differentiated from adipose-derived mesenchymal stem cells on three different scaffolds: an animal study. Cell Tissue Bank 2016;17:69-83. DOI: 10.1007/s10561-015-9518-5
  • Yun JH, Yoo JH, Choi SH, Lee MH, Lee SJ, Song SU, O NS. Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits. Tissue Eng Regen Med. 2012;9(1):17-23. DOI: 10.1007/s13770-012-0017-5
  • Wiltfang J, Merten HA, Schlegel KA, Schultze-Mosgau S, Kloss FR, Rupprecht S, Kessler P. Degradation characteristics of α and β tri-calcium-phosphate (TCP) in minipigs. J Biomed Mat Res. 2002;63(2):115-121. DOI: 10.1002/jbm.10084
  • Yuan J, Cui L, Zhang WJ, Liu W, Cao Y. Repair of canine mandibular bone defects with bone marrow stromal cells and porous β-tricalcium phosphate. Biomaterials 2007;28(6): 1005-1013. DOI: 10.1016/j.biomaterials.2006.10.015
  • Borrell LN, Papapanou PN. Analytical epidemiology of periodontitis. J Clin Periodontol. 2005;32(6):132-158. DOI: 10.1111/j.1600-051X.2005.00799.x
  • Kuo LC, Polson AM, Kang T. Associations between periodontal diseases and systemic diseases: a review of the inter-relationships and interactions with diabetes, respiratory diseases, cardiovascular diseases and osteoporosis. Public Health 2008; 122(4):417-433. DOI: 10.1016/j.puhe.2007.07.004
  • Follak N, Klöting I. Merk H. Influence of diabetic metabolic state on fracture healing in spontaneously diabetic rats. Diabetes Metab Res Rev. 2005;21(3):288-296. DOI: 10.1002/dmrr.537
  • Rakel A, Sheehy O, Rahme E, LeLorier J. Osteoporosis among patients with type 1 and type 2 diabetes. Diabetes Metab. 2008;34(3):193-205. DOI: 10.1016/j.diabet.2007.10.008
  • Goker H, Haznedaroglu IC, Ercetin S, Kirazlı S, Akman U, Ozturk Y, Firat HC. Haemostatic actions of the folkloric medicinal plant extract ankaferd blood stopper. J Int Med Res. 2008;36(1):163-170.
  • Odabaş ME, Ertürk M, Çınar Ç, Tüzüner T, Tulunoğlu O. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Pathol Oral Cir Bucal. 2011; 16(4): 584-587. DOI: 10.4317/medoral.16.e584
  • Işler SC, Demircan S, Cakarer S, Cebi Z, Keskin C, Soluk M, Yüzbaşioğlu E. Effects of folk medicinal plant extract ankaferd blood stopper on early bone healing. J Appl Oral Sci. 2010;18: 409-414. DOI: 10.1590/S1678-77572010000400015
  • Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL. Regeneration of periodontal tissue: bone replacement grafts. Dent Clin North Am. 2010;54(1): 55-71. DOI: 10.1016/j.cden.2009.09.003
  • Rojbani H, Nyan M, Ohya K, Kasugai S. Evaluation of the osteoconductivity of α-tricalcium phosphate, β‐tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J Biomed Mater Res Part A. 2011;98(4): 488-498. DOI: 10.1002/jbm.a.33117
  • Campus G, Salem A, Uzzau S, Baldoni E, Tonolo G. Diabetes and periodontal disease: a case-control study. J Periodontol. 2005;76:418-425. DOI: 10.1902/jop.2005.76.3.418
  • Schwartz AV, Garnero P, Hillier TA, Sellmeyer DE, Strotmeyer ES, Feingold KR, Resnick HE, Tylavsky FA, Black DM, Cummings SR, Harris TB, Bauer DC. Pentosidine and increased fracture risk in older adults with type 2 diabetes. J Clin Endocrinol Metab. 2009;94(7):2380-2386. DOI: 10.1210/jc.2008-2498
  • Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relationship of dose to metabolic response. J Clin Invest. 1969; 48(11):2129-2139. DOI: 10.1172/JCI106180.
  • Benwahhoud M, Jouad H, Eddouks M, Lyoussi B. Hypoglycemic effect of suaeda fruticosa in streptozotocin induced diabetic rats. J Rethnopharmacol. 2001;76(1):35-8. DOI: 10.1016/S0378-8741(01)00207-0
  • Firat HC, Ozdemir O, Kosar A, Goker H, Haznedaroglu IC. Annual review of ankaferd 08-09. 1st ed. Istanbul: Naviga Publications; 2009.
  • Tek M, Akkas I, Toptas O, Ozan F, Sener I, Bereket C. Effects of the topical hemostatic agent ankaferd blood stopper on the incidence of alveolar osteitis after surgical removal of an impacted mandibular third molar. Niger J Clin Pract. 2014;17(1):75-80. DOI: 10.4103/1119-3077.122847
  • Tanik A, Doğru AG, Akpolat V, Kaya FA, Sarıbaş E, Gül M, İrtegün SK, Deveci E. Investigation of the effect of combined use of alloplastic-based tricalcium phosphate bone graft and antihemorrhagic plant extract (ABS) on bone regeneration in surgically induced bone defects in nondiabetic rats: an experimental animal study. Turk J Med Sci. 2018; 48(6), 1302-1314. DOI: 10.3906/sag-1803-201
  • Bulut E, Baş B, Altunkaynak, BZ, Bekçioğlu B, Erdem GK, Gönülol E, Önger ME, Kaplan S. Efficacy of ankaferd blood stopper on bone healing in diabetic rats: A stereological and histopathological study. Biotech Histochem. 2014;89(7):535-543. DOI: 10.3109/10520295.2014.906657
  • Alpan AL, Toker H, Ozer H. Ozone therapy enhances osseous healing in rats with diabetes with calvarial defects: a morphometric and ımmunohistochemical study. J periodontol. 2016;87(8):982-989. DOI: 10.1902/jop.2016.160009
  • Sodek J, Ganss B, McKee MD. Osteopontin. Crit Rev Oral Biol Med. 2000;11(3):279-303. DOI: 10.1177/10454411000110030101
  • Romanowski R, Jundt G, Termine JD, von der Mark K, Schulz A. Immunoelectron microscopy of osteonectin and type I collagen in osteoblasts and bone matrix. Calcif Tissue Int. 1990;46(6): 353-360. DOI: 10.1007/BF02554964
  • Fındık Y, Timuçin B. Effects of low-intensity pulsed ultrasound on autogenous bone graft healing. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014;117(3): 255-260. DOI: 10.1016/j.oooo.2012.05.023
  • Barou O, Valentin D, Vico L, Tirode C, Barbier A, Alexandre C, Lafage-Proust MH. High-resolution three-dimensional micro-computed tomography detects bone loss and changes in trabecular architecture early: comparison with DEXA and bone histomorphometry in a rat model of disuse osteoporosis. Invest Radiol. 2002;37(1):40-46.
  • AboElAsrar MA, Elbarbary NS, Elshennawy DE, Omar AM. Insulin-like growth factor-1 cytokines cross-talk in type 1 diabetes mellitus: relationship to microvascular complications and bone mineral density. Cytokine2012;59 (1): 86-93. DOI: 10.1016/j.cyto.2012.03.019
  • Duarte, VMG, Ramos AM, Rezende LA, Macedo UB, Brandão-Neto J, Almeida MG, Rezende AA. Osteopenia: A bone disorder associated with diabetes mellitus. J Bone Miner Metab. 2005;23 (1): 58-68. DOI: 10.1007/s00774-004-0542-y

Year 2023, , 308 - 315, 15.06.2023

Abdulsamet Tanık , Arzum Güler Dogru , Mehmet Gül

https://doi.org/10.33808/clinexphealthsci.1080508

Abstract

Project Number

DİŞ.15.008

References

  • Detert J, Pischon N, Burmester GR, Buttgereit F. The association between rheumatoid arthritis and periodontal disease. Arthritis Res Ther. 2010;12(5):218. DOI: 10.1186/ar3106
  • Bascones-Martinez A, Matesanz-Perez P, Escribano-Bermejo M, González-Moles MÁ, Bascones-Ilundain J, Meurman JH. Periodontal disease and diabetes-review of the literature. Med Oral Patol Oral Cir Bucal. 2011;16(6):722-729. DOI:10.4317/medoral.17032
  • Semyari H, Rajipour M, Sabetkish S, Sabetkish N, Mashhadi AF, Kajbafzadeh AM. Evaluating the bone regeneration in calvarial defect using osteoblasts differentiated from adipose-derived mesenchymal stem cells on three different scaffolds: an animal study. Cell Tissue Bank 2016;17:69-83. DOI: 10.1007/s10561-015-9518-5
  • Yun JH, Yoo JH, Choi SH, Lee MH, Lee SJ, Song SU, O NS. Synergistic effect of bone marrow-derived mesenchymal stem cells and platelet-rich plasma on bone regeneration of calvarial defects in rabbits. Tissue Eng Regen Med. 2012;9(1):17-23. DOI: 10.1007/s13770-012-0017-5
  • Wiltfang J, Merten HA, Schlegel KA, Schultze-Mosgau S, Kloss FR, Rupprecht S, Kessler P. Degradation characteristics of α and β tri-calcium-phosphate (TCP) in minipigs. J Biomed Mat Res. 2002;63(2):115-121. DOI: 10.1002/jbm.10084
  • Yuan J, Cui L, Zhang WJ, Liu W, Cao Y. Repair of canine mandibular bone defects with bone marrow stromal cells and porous β-tricalcium phosphate. Biomaterials 2007;28(6): 1005-1013. DOI: 10.1016/j.biomaterials.2006.10.015
  • Borrell LN, Papapanou PN. Analytical epidemiology of periodontitis. J Clin Periodontol. 2005;32(6):132-158. DOI: 10.1111/j.1600-051X.2005.00799.x
  • Kuo LC, Polson AM, Kang T. Associations between periodontal diseases and systemic diseases: a review of the inter-relationships and interactions with diabetes, respiratory diseases, cardiovascular diseases and osteoporosis. Public Health 2008; 122(4):417-433. DOI: 10.1016/j.puhe.2007.07.004
  • Follak N, Klöting I. Merk H. Influence of diabetic metabolic state on fracture healing in spontaneously diabetic rats. Diabetes Metab Res Rev. 2005;21(3):288-296. DOI: 10.1002/dmrr.537
  • Rakel A, Sheehy O, Rahme E, LeLorier J. Osteoporosis among patients with type 1 and type 2 diabetes. Diabetes Metab. 2008;34(3):193-205. DOI: 10.1016/j.diabet.2007.10.008
  • Goker H, Haznedaroglu IC, Ercetin S, Kirazlı S, Akman U, Ozturk Y, Firat HC. Haemostatic actions of the folkloric medicinal plant extract ankaferd blood stopper. J Int Med Res. 2008;36(1):163-170.
  • Odabaş ME, Ertürk M, Çınar Ç, Tüzüner T, Tulunoğlu O. Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro. Med Oral Pathol Oral Cir Bucal. 2011; 16(4): 584-587. DOI: 10.4317/medoral.16.e584
  • Işler SC, Demircan S, Cakarer S, Cebi Z, Keskin C, Soluk M, Yüzbaşioğlu E. Effects of folk medicinal plant extract ankaferd blood stopper on early bone healing. J Appl Oral Sci. 2010;18: 409-414. DOI: 10.1590/S1678-77572010000400015
  • Reynolds MA, Aichelmann-Reidy ME, Branch-Mays GL. Regeneration of periodontal tissue: bone replacement grafts. Dent Clin North Am. 2010;54(1): 55-71. DOI: 10.1016/j.cden.2009.09.003
  • Rojbani H, Nyan M, Ohya K, Kasugai S. Evaluation of the osteoconductivity of α-tricalcium phosphate, β‐tricalcium phosphate, and hydroxyapatite combined with or without simvastatin in rat calvarial defect. J Biomed Mater Res Part A. 2011;98(4): 488-498. DOI: 10.1002/jbm.a.33117
  • Campus G, Salem A, Uzzau S, Baldoni E, Tonolo G. Diabetes and periodontal disease: a case-control study. J Periodontol. 2005;76:418-425. DOI: 10.1902/jop.2005.76.3.418
  • Schwartz AV, Garnero P, Hillier TA, Sellmeyer DE, Strotmeyer ES, Feingold KR, Resnick HE, Tylavsky FA, Black DM, Cummings SR, Harris TB, Bauer DC. Pentosidine and increased fracture risk in older adults with type 2 diabetes. J Clin Endocrinol Metab. 2009;94(7):2380-2386. DOI: 10.1210/jc.2008-2498
  • Junod A, Lambert AE, Stauffacher W, Renold AE. Diabetogenic action of streptozotocin: Relationship of dose to metabolic response. J Clin Invest. 1969; 48(11):2129-2139. DOI: 10.1172/JCI106180.
  • Benwahhoud M, Jouad H, Eddouks M, Lyoussi B. Hypoglycemic effect of suaeda fruticosa in streptozotocin induced diabetic rats. J Rethnopharmacol. 2001;76(1):35-8. DOI: 10.1016/S0378-8741(01)00207-0
  • Firat HC, Ozdemir O, Kosar A, Goker H, Haznedaroglu IC. Annual review of ankaferd 08-09. 1st ed. Istanbul: Naviga Publications; 2009.
  • Tek M, Akkas I, Toptas O, Ozan F, Sener I, Bereket C. Effects of the topical hemostatic agent ankaferd blood stopper on the incidence of alveolar osteitis after surgical removal of an impacted mandibular third molar. Niger J Clin Pract. 2014;17(1):75-80. DOI: 10.4103/1119-3077.122847
  • Tanik A, Doğru AG, Akpolat V, Kaya FA, Sarıbaş E, Gül M, İrtegün SK, Deveci E. Investigation of the effect of combined use of alloplastic-based tricalcium phosphate bone graft and antihemorrhagic plant extract (ABS) on bone regeneration in surgically induced bone defects in nondiabetic rats: an experimental animal study. Turk J Med Sci. 2018; 48(6), 1302-1314. DOI: 10.3906/sag-1803-201
  • Bulut E, Baş B, Altunkaynak, BZ, Bekçioğlu B, Erdem GK, Gönülol E, Önger ME, Kaplan S. Efficacy of ankaferd blood stopper on bone healing in diabetic rats: A stereological and histopathological study. Biotech Histochem. 2014;89(7):535-543. DOI: 10.3109/10520295.2014.906657
  • Alpan AL, Toker H, Ozer H. Ozone therapy enhances osseous healing in rats with diabetes with calvarial defects: a morphometric and ımmunohistochemical study. J periodontol. 2016;87(8):982-989. DOI: 10.1902/jop.2016.160009
  • Sodek J, Ganss B, McKee MD. Osteopontin. Crit Rev Oral Biol Med. 2000;11(3):279-303. DOI: 10.1177/10454411000110030101
  • Romanowski R, Jundt G, Termine JD, von der Mark K, Schulz A. Immunoelectron microscopy of osteonectin and type I collagen in osteoblasts and bone matrix. Calcif Tissue Int. 1990;46(6): 353-360. DOI: 10.1007/BF02554964
  • Fındık Y, Timuçin B. Effects of low-intensity pulsed ultrasound on autogenous bone graft healing. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014;117(3): 255-260. DOI: 10.1016/j.oooo.2012.05.023
  • Barou O, Valentin D, Vico L, Tirode C, Barbier A, Alexandre C, Lafage-Proust MH. High-resolution three-dimensional micro-computed tomography detects bone loss and changes in trabecular architecture early: comparison with DEXA and bone histomorphometry in a rat model of disuse osteoporosis. Invest Radiol. 2002;37(1):40-46.
  • AboElAsrar MA, Elbarbary NS, Elshennawy DE, Omar AM. Insulin-like growth factor-1 cytokines cross-talk in type 1 diabetes mellitus: relationship to microvascular complications and bone mineral density. Cytokine2012;59 (1): 86-93. DOI: 10.1016/j.cyto.2012.03.019
  • Duarte, VMG, Ramos AM, Rezende LA, Macedo UB, Brandão-Neto J, Almeida MG, Rezende AA. Osteopenia: A bone disorder associated with diabetes mellitus. J Bone Miner Metab. 2005;23 (1): 58-68. DOI: 10.1007/s00774-004-0542-y
There are 30 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Abdulsamet Tanık 0000-0002-4430-2196

Arzum Güler Dogru 0000-0001-6976-2589

Mehmet Gül 0000-0002-5721-8778

Project Number DİŞ.15.008
Publication Date June 15, 2023
Submission Date March 9, 2022
Published in Issue Year 2023

Cite

APA Tanık, A., Dogru, A. G., & Gül, M. (2023). Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats. Clinical and Experimental Health Sciences, 13(2), 308-315. https://doi.org/10.33808/clinexphealthsci.1080508
AMA Tanık A, Dogru AG, Gül M. Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats. Clinical and Experimental Health Sciences. June 2023;13(2):308-315. doi:10.33808/clinexphealthsci.1080508
Chicago Tanık, Abdulsamet, Arzum Güler Dogru, and Mehmet Gül. “Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats”. Clinical and Experimental Health Sciences 13, no. 2 (June 2023): 308-15. https://doi.org/10.33808/clinexphealthsci.1080508.
EndNote Tanık A, Dogru AG, Gül M (June 1, 2023) Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats. Clinical and Experimental Health Sciences 13 2 308–315.
IEEE A. Tanık, A. G. Dogru, and M. Gül, “Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats”, Clinical and Experimental Health Sciences, vol. 13, no. 2, pp. 308–315, 2023, doi: 10.33808/clinexphealthsci.1080508.
ISNAD Tanık, Abdulsamet et al. “Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats”. Clinical and Experimental Health Sciences 13/2 (June 2023), 308-315. https://doi.org/10.33808/clinexphealthsci.1080508.
JAMA Tanık A, Dogru AG, Gül M. Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats. Clinical and Experimental Health Sciences. 2023;13:308–315.
MLA Tanık, Abdulsamet et al. “Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats”. Clinical and Experimental Health Sciences, vol. 13, no. 2, 2023, pp. 308-15, doi:10.33808/clinexphealthsci.1080508.
Vancouver Tanık A, Dogru AG, Gül M. Evaluating the Effect of Jointly Administering Synthetic Graft and Ankaferd Blood Stopper for Bone Recovery in Created Bone Deformities in Diabetic Rats. Clinical and Experimental Health Sciences. 2023;13(2):308-15.

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