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Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats

Year 2023, , 555 - 561, 11.09.2023

Canan Çulha , Zeynep Ülkü Gün , Aysu Selçuk , Şule Apikoğlu-rabuş

https://doi.org/10.33808/clinexphealthsci.1112790

Abstract

Objective: People have used traditional herbal medicines for wound care since the dawn of time. This study aimed to assess the cutaneous wound healing effects of wheat germ oil (WGO) and quince seed mucilage (QSM) in rats.
Methods: Adult female Wistar albino rats were allocated to one of the three groups: rats treated with topical WGO (n=6); topical QSM (n=6); and topical saline (n=6) as the control group. Two circular, full-thickness wounds of 0.6 mm diameter were created on the dorsal thoracic region of each rat. Test and control solutions were applied twice daily for 14 days. Wound healing was assessed by measuring the wound contraction rate and the time needed for complete epithelialization.
Results: When compared with the control group, rats in the WGO group had reduced wound closure rates in the first four days, but considerably greater rates in the 8th, 10th, and 12th days, as well as a shorter duration of time needed to complete epithelialization (11 days vs. 13 days). The wound closure rates of the rats in the QSM group were not substantially different from the control rats and the duration of time needed for complete epithelialization was not significantly different from the control group.
Conclusion: WGO use has been shown to improve wound healing. It may be used as an alternative or complementary approach for wound treatment depending on the severity of the wounds. On the other hand, QSM was not found to improve wound healing.

Keywords

Wound healing wheat germ oil quince seed mucilage

References

  • Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc. 2018;81(2):94-101. DOI: 10.1016/j.jcma.2017.11.002
  • Williams JZ, Barbul A. Nutrition and wound healing. Crit Care Nurs Clin North Am. 2012;24(2):179-200. DOI: 10.1016/j.ccell.2012.03.001
  • Hamidi SA, Tabatabaei Naeini A, Oryan A, Tabandeh MR, Tanideh N, Nazifi S. Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turk J Pharm Sci. 2017;14(1):65-74. DOI: 10.4274/tjps.41713
  • Lingaraju GM, Krishna V, Joy Hoskeri H, Pradeepa K, Venkatesh, Babu PS. Wound healing promoting activity of stem bark extract of Semecarpus anacardium using rats. Nat Prod Res. 2012;26(24):2344-2347. DOI: 10.1080/14786419.2012.656108
  • Arshad MS, Anjum FM, Khan MI, Shahid M, Akhtar S, Sohaib M. Wheat germ oil enrichment in broiler feed with α-lipoic acid to enhance the antioxidant potential and lipid stability of meat. Lipids Health Dis. 2013;12:164. DOI: 10.1186/1476-511X-12-164
  • Magalhães AS, Silva BM, Pereira JA, Andrade PB, Valentão P, Carvalho M. Protective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytes. Food Chem Toxicol. 2009;47(6):1372-1377. DOI: 10.1016/j.fct.2009.03.017
  • Hemmati AA, Kalantari H, Jalali A, Rezai S, Zadeh HH. Healing effect of quince seed mucilage on T-2 toxin-induced dermal toxicity in rabbit. Exp Toxicol Pathol. 2012;64(3):181-186. DOI: 10.1016/j.etp.2010.08.004
  • Apikoglu-Rabus S, Izzettin FV, Turan P, Ercan F. Effect of topical insulin on cutaneous wound healing in rats with or without acute diabetes. Clin Exp Dermatol. 2010;35(2):180-185. DOI: 10.1111/j.1365-2230.2009.03419.x
  • Sui H, Wang F, Weng Z, Song H, Fang Y, Tang X, Shen X. A wheat germ-derived peptide YDWPGGRN facilitates skin wound-healing processes. Biochem Biophys Res Commun. 2020;524(4):943-950. DOI: 10.1016/j.bbrc.2020.01.162
  • Weimann E, Silva MBB, Murata GM, Bortolon JR, Dermargos A, Curi R, Hatanaka E. Topical anti-inflammatory activity of palmitoleic acid improves wound healing. PLoS One. 2018;13(10): e0205338. DOI: 10.1371/journal.pone.0205338
  • Bardania H, Mahmoudi R, Bagheri H, Salehpour Z, Fouani MH, Darabian B, Khoramrooz SS, Mousavizadeh A, Kowsari M, Moosavifard SE, Christiansen G, Javeshghani D, Alipour M, Akrami M. Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing. Sci Rep. 2020;10(1): 6129. DOI: 10.1038/s41598-020-63032-5
  • Zou Y, Gao Y, He H, Yang T. Effect of roasting on physico-chemical properties, antioxidant capacity, and oxidative stability of wheat germ oil. LWT. 2018;90:246-253. DOI: 10.1016/j.lwt.2017.12.038
  • Kumar GS, Krishna AG. Studies on the nutraceuticals composition of wheat derived oils wheat bran oil and wheat germ oil. J Food Sci Technol. 2015;52(2):1145-1151. DOI: 10.1007/s13197-013-1119-3
  • Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair Regen. 2004;12(2):235-243. DOI: 10.1111/j.1067-1927.2004.012216.x
  • Pereira LM, Hatanaka E, Martins EF, Oliveira F, Liberti EA, Farsky SH, Curi R, Pithon-Curi TC. Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem Funct. 2008;26(2):197-204. DOI: 10.1002/cbf.1432
  • Magalhães MS, Fechine FV, Macedo RN, Monteiro DL, Oliveira CC, Brito GA, Moraes ME, Moraes MO. Effect of a combination of medium chain triglycerides, linoleic acid, soy lecithin and vitamins A and E on wound healing in rats. Acta Cir Bras. 2008;23(3):262-269. DOI: 10.1590/s0102-86502008000300009
  • Zimmer MM, Frank J, Barker JH, Becker H. Effect of extracts from the Chinese and European mole cricket on wound epithelialization and neovascularization: in vivo studies in the hairless mouse ear wound model. Wound Repair Regen. 2006;14(2):142-151. DOI: 10.1111/j.1743-6109.2006.00104.x
  • Calder PC. Polyunsaturated fatty acids and inflammatory processes: New twists in an old tale. Biochimie. 2009;91(6):791-795. DOI: 10.1016/j.biochi.2009.01.008
  • Cardoso CR, Favoreto S Jr, Oliveira LL, Vancim JO, Barban GB, Ferraz DB, Silva JS. Oleic acid modulation of the immune response in wound healing: a new approach for skin repair. Immunobiology. 2011;216(3):409-415. DOI: 10.1016/j.imbio.2010.06.007
  • Zampieri N, Zuin V, Burro R, Ottolenghi A, Camoglio FS. A prospective study in children: Pre- and post-surgery use of vitamin E in surgical incisions. J Plast Reconstr Aesthet Surg. 2010;63(9):1474-1478. DOI: 10.1016/j.bjps.2009.08.018
  • Lin TS, Abd Latiff A, Abd Hamid NA, Wan Ngah WZ, Mazlan M. Evaluation of topical tocopherol cream on cutaneous wound healing in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med. 2012;2012:491027. DOI: 10.1155/2012/491027
  • Leenhardt F, Fardet A, Lyan B, Gueux E, Rock E, Mazur A, Chanliaud E, Demigné C, Rémésy C. Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. J Am Coll Nutr. 2008;27(2):222-228. DOI: 10.1080/07315724.2008.10719694
  • Kmiecik D, Korczak J, Rudzińska M, Kobus-Cisowska J, Gramza-Michałowska A, Hęś M. β-Sitosterol and campesterol stabilisation by natural and synthetic antioxidants during heating. Food Chem. 2011;128(4):937-942. DOI:10.1016/j.foodchem.2011.03.122
  • Bhat AH, Alia A, Rather GM, Kumar B. Isolation and Characterisation of Beta-sitosterol from the rhizomes of Arisaema utile and its Evaluation for Antioxidant Activity. Int. J. Sci. Res. Biol. Sci. 2019;6(2):111-118. DOI:10.26438/ijsrbs/v6i2.111118
  • Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, Herrera-López B, Cervantes-Hernández I, Madrigal-Santillán O, Morales-González JA, Álvarez-González I, Madrigal-Bujaidar E. Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays. Afr J Tradit Complement Altern Med. 2017;14(1):123-130. DOI: 10.21010/ajtcam.v14i1.13
  • Valerio M, Awad AB. beta-Sitosterol down-regulates some pro-inflammatory signal transduction pathways by increasing the activity of tyrosine phosphatase SHP-1 in J774A.1 murine macrophages. Int Immunopharmacol. 2011;11(8):1012-1017. DOI: 10.1016/j.intimp.2011.02.018
  • Jewo PI, Fadeyibi IO, Babalola OS, Saalu LC, Benebo AS, Izegbu MC, Ashiru OA. A Comparative Study of the Wound Healing Properties of Moist Exposed Burn Ointment (MEBO) and Silver Sulphadiazine. Ann Burns Fire Disasters. 2009;22(2):79-82.
  • Brandolini A, Hidalgo A. Wheat germ: not only a by-product. Int J Food Sci Nutr. 2012;63 Suppl 1:71-74. DOI: 10.3109/09637486.2011.633898
  • Ghafourian M, Tamri P, Hemmati A. Enhancement of human skin fibroblasts proliferation as a result of treating with quince seed mucilage. Jundishapur J Nat Pharm Prod. 2015;10(1):e18820. DOI: 10.17795/jjnpp-18820
  • Izadyari Aghmiuni A, Heidari Keshel S, Sefat F, Akbarzadeh Khiyavi A. Quince seed mucilage-based scaffold as a smart biological substrate to mimic mechanobiological behavior of skin and promote fibroblasts proliferation and h-ASCs differentiation into keratinocytes. Int J Biol Macromol. 2020;142:668-679. DOI: 10.1016/j.ijbiomac.2019.10.008
  • Tamri P, Hemmati A, Boroujerdnia MG. Wound healing properties of quince seed mucilage: in vivo evaluation in rabbit full-thickness wound model. Int J Surg. 2014;12(8):843-847. DOI: 10.1016/j.ijsu.2014.06.016
  • Ousey K, Cutting KF, Rogers AA, Rippon MG. The importance of hydration in wound healing: reinvigorating the clinical perspective. J Wound Care. 2016;25(3):122, 4-30. DOI: 10.12968/jowc.2016.25.3.122

Year 2023, , 555 - 561, 11.09.2023

Canan Çulha , Zeynep Ülkü Gün , Aysu Selçuk , Şule Apikoğlu-rabuş

https://doi.org/10.33808/clinexphealthsci.1112790

Abstract

References

  • Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc. 2018;81(2):94-101. DOI: 10.1016/j.jcma.2017.11.002
  • Williams JZ, Barbul A. Nutrition and wound healing. Crit Care Nurs Clin North Am. 2012;24(2):179-200. DOI: 10.1016/j.ccell.2012.03.001
  • Hamidi SA, Tabatabaei Naeini A, Oryan A, Tabandeh MR, Tanideh N, Nazifi S. Cutaneous Wound Healing after Topical Application of Pistacia atlantica Gel Formulation in Rats. Turk J Pharm Sci. 2017;14(1):65-74. DOI: 10.4274/tjps.41713
  • Lingaraju GM, Krishna V, Joy Hoskeri H, Pradeepa K, Venkatesh, Babu PS. Wound healing promoting activity of stem bark extract of Semecarpus anacardium using rats. Nat Prod Res. 2012;26(24):2344-2347. DOI: 10.1080/14786419.2012.656108
  • Arshad MS, Anjum FM, Khan MI, Shahid M, Akhtar S, Sohaib M. Wheat germ oil enrichment in broiler feed with α-lipoic acid to enhance the antioxidant potential and lipid stability of meat. Lipids Health Dis. 2013;12:164. DOI: 10.1186/1476-511X-12-164
  • Magalhães AS, Silva BM, Pereira JA, Andrade PB, Valentão P, Carvalho M. Protective effect of quince (Cydonia oblonga Miller) fruit against oxidative hemolysis of human erythrocytes. Food Chem Toxicol. 2009;47(6):1372-1377. DOI: 10.1016/j.fct.2009.03.017
  • Hemmati AA, Kalantari H, Jalali A, Rezai S, Zadeh HH. Healing effect of quince seed mucilage on T-2 toxin-induced dermal toxicity in rabbit. Exp Toxicol Pathol. 2012;64(3):181-186. DOI: 10.1016/j.etp.2010.08.004
  • Apikoglu-Rabus S, Izzettin FV, Turan P, Ercan F. Effect of topical insulin on cutaneous wound healing in rats with or without acute diabetes. Clin Exp Dermatol. 2010;35(2):180-185. DOI: 10.1111/j.1365-2230.2009.03419.x
  • Sui H, Wang F, Weng Z, Song H, Fang Y, Tang X, Shen X. A wheat germ-derived peptide YDWPGGRN facilitates skin wound-healing processes. Biochem Biophys Res Commun. 2020;524(4):943-950. DOI: 10.1016/j.bbrc.2020.01.162
  • Weimann E, Silva MBB, Murata GM, Bortolon JR, Dermargos A, Curi R, Hatanaka E. Topical anti-inflammatory activity of palmitoleic acid improves wound healing. PLoS One. 2018;13(10): e0205338. DOI: 10.1371/journal.pone.0205338
  • Bardania H, Mahmoudi R, Bagheri H, Salehpour Z, Fouani MH, Darabian B, Khoramrooz SS, Mousavizadeh A, Kowsari M, Moosavifard SE, Christiansen G, Javeshghani D, Alipour M, Akrami M. Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing. Sci Rep. 2020;10(1): 6129. DOI: 10.1038/s41598-020-63032-5
  • Zou Y, Gao Y, He H, Yang T. Effect of roasting on physico-chemical properties, antioxidant capacity, and oxidative stability of wheat germ oil. LWT. 2018;90:246-253. DOI: 10.1016/j.lwt.2017.12.038
  • Kumar GS, Krishna AG. Studies on the nutraceuticals composition of wheat derived oils wheat bran oil and wheat germ oil. J Food Sci Technol. 2015;52(2):1145-1151. DOI: 10.1007/s13197-013-1119-3
  • Cardoso CR, Souza MA, Ferro EA, Favoreto S Jr, Pena JD. Influence of topical administration of n-3 and n-6 essential and n-9 nonessential fatty acids on the healing of cutaneous wounds. Wound Repair Regen. 2004;12(2):235-243. DOI: 10.1111/j.1067-1927.2004.012216.x
  • Pereira LM, Hatanaka E, Martins EF, Oliveira F, Liberti EA, Farsky SH, Curi R, Pithon-Curi TC. Effect of oleic and linoleic acids on the inflammatory phase of wound healing in rats. Cell Biochem Funct. 2008;26(2):197-204. DOI: 10.1002/cbf.1432
  • Magalhães MS, Fechine FV, Macedo RN, Monteiro DL, Oliveira CC, Brito GA, Moraes ME, Moraes MO. Effect of a combination of medium chain triglycerides, linoleic acid, soy lecithin and vitamins A and E on wound healing in rats. Acta Cir Bras. 2008;23(3):262-269. DOI: 10.1590/s0102-86502008000300009
  • Zimmer MM, Frank J, Barker JH, Becker H. Effect of extracts from the Chinese and European mole cricket on wound epithelialization and neovascularization: in vivo studies in the hairless mouse ear wound model. Wound Repair Regen. 2006;14(2):142-151. DOI: 10.1111/j.1743-6109.2006.00104.x
  • Calder PC. Polyunsaturated fatty acids and inflammatory processes: New twists in an old tale. Biochimie. 2009;91(6):791-795. DOI: 10.1016/j.biochi.2009.01.008
  • Cardoso CR, Favoreto S Jr, Oliveira LL, Vancim JO, Barban GB, Ferraz DB, Silva JS. Oleic acid modulation of the immune response in wound healing: a new approach for skin repair. Immunobiology. 2011;216(3):409-415. DOI: 10.1016/j.imbio.2010.06.007
  • Zampieri N, Zuin V, Burro R, Ottolenghi A, Camoglio FS. A prospective study in children: Pre- and post-surgery use of vitamin E in surgical incisions. J Plast Reconstr Aesthet Surg. 2010;63(9):1474-1478. DOI: 10.1016/j.bjps.2009.08.018
  • Lin TS, Abd Latiff A, Abd Hamid NA, Wan Ngah WZ, Mazlan M. Evaluation of topical tocopherol cream on cutaneous wound healing in streptozotocin-induced diabetic rats. Evid Based Complement Alternat Med. 2012;2012:491027. DOI: 10.1155/2012/491027
  • Leenhardt F, Fardet A, Lyan B, Gueux E, Rock E, Mazur A, Chanliaud E, Demigné C, Rémésy C. Wheat germ supplementation of a low vitamin E diet in rats affords effective antioxidant protection in tissues. J Am Coll Nutr. 2008;27(2):222-228. DOI: 10.1080/07315724.2008.10719694
  • Kmiecik D, Korczak J, Rudzińska M, Kobus-Cisowska J, Gramza-Michałowska A, Hęś M. β-Sitosterol and campesterol stabilisation by natural and synthetic antioxidants during heating. Food Chem. 2011;128(4):937-942. DOI:10.1016/j.foodchem.2011.03.122
  • Bhat AH, Alia A, Rather GM, Kumar B. Isolation and Characterisation of Beta-sitosterol from the rhizomes of Arisaema utile and its Evaluation for Antioxidant Activity. Int. J. Sci. Res. Biol. Sci. 2019;6(2):111-118. DOI:10.26438/ijsrbs/v6i2.111118
  • Paniagua-Pérez R, Flores-Mondragón G, Reyes-Legorreta C, Herrera-López B, Cervantes-Hernández I, Madrigal-Santillán O, Morales-González JA, Álvarez-González I, Madrigal-Bujaidar E. Evaluation of the Anti-Inflammatory Capacity of Beta-Sitosterol in Rodent Assays. Afr J Tradit Complement Altern Med. 2017;14(1):123-130. DOI: 10.21010/ajtcam.v14i1.13
  • Valerio M, Awad AB. beta-Sitosterol down-regulates some pro-inflammatory signal transduction pathways by increasing the activity of tyrosine phosphatase SHP-1 in J774A.1 murine macrophages. Int Immunopharmacol. 2011;11(8):1012-1017. DOI: 10.1016/j.intimp.2011.02.018
  • Jewo PI, Fadeyibi IO, Babalola OS, Saalu LC, Benebo AS, Izegbu MC, Ashiru OA. A Comparative Study of the Wound Healing Properties of Moist Exposed Burn Ointment (MEBO) and Silver Sulphadiazine. Ann Burns Fire Disasters. 2009;22(2):79-82.
  • Brandolini A, Hidalgo A. Wheat germ: not only a by-product. Int J Food Sci Nutr. 2012;63 Suppl 1:71-74. DOI: 10.3109/09637486.2011.633898
  • Ghafourian M, Tamri P, Hemmati A. Enhancement of human skin fibroblasts proliferation as a result of treating with quince seed mucilage. Jundishapur J Nat Pharm Prod. 2015;10(1):e18820. DOI: 10.17795/jjnpp-18820
  • Izadyari Aghmiuni A, Heidari Keshel S, Sefat F, Akbarzadeh Khiyavi A. Quince seed mucilage-based scaffold as a smart biological substrate to mimic mechanobiological behavior of skin and promote fibroblasts proliferation and h-ASCs differentiation into keratinocytes. Int J Biol Macromol. 2020;142:668-679. DOI: 10.1016/j.ijbiomac.2019.10.008
  • Tamri P, Hemmati A, Boroujerdnia MG. Wound healing properties of quince seed mucilage: in vivo evaluation in rabbit full-thickness wound model. Int J Surg. 2014;12(8):843-847. DOI: 10.1016/j.ijsu.2014.06.016
  • Ousey K, Cutting KF, Rogers AA, Rippon MG. The importance of hydration in wound healing: reinvigorating the clinical perspective. J Wound Care. 2016;25(3):122, 4-30. DOI: 10.12968/jowc.2016.25.3.122
There are 32 citations in total.

Details

Primary Language English
Subjects Clinical Pharmacy and Pharmacy Practice
Journal Section Articles
Authors

Canan Çulha 0000-0002-7714-7444

Zeynep Ülkü Gün 0000-0002-7131-6585

Aysu Selçuk 0000-0001-6424-7457

Şule Apikoğlu-rabuş 0000-0001-9137-4865

Publication Date September 11, 2023
Submission Date May 5, 2022
Published in Issue Year 2023

Cite

APA Çulha, C., Gün, Z. Ü., Selçuk, A., Apikoğlu-rabuş, Ş. (2023). Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats. Clinical and Experimental Health Sciences, 13(3), 555-561. https://doi.org/10.33808/clinexphealthsci.1112790
AMA Çulha C, Gün ZÜ, Selçuk A, Apikoğlu-rabuş Ş. Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats. Clinical and Experimental Health Sciences. September 2023;13(3):555-561. doi:10.33808/clinexphealthsci.1112790
Chicago Çulha, Canan, Zeynep Ülkü Gün, Aysu Selçuk, and Şule Apikoğlu-rabuş. “Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats”. Clinical and Experimental Health Sciences 13, no. 3 (September 2023): 555-61. https://doi.org/10.33808/clinexphealthsci.1112790.
EndNote Çulha C, Gün ZÜ, Selçuk A, Apikoğlu-rabuş Ş (September 1, 2023) Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats. Clinical and Experimental Health Sciences 13 3 555–561.
IEEE C. Çulha, Z. Ü. Gün, A. Selçuk, and Ş. Apikoğlu-rabuş, “Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats”, Clinical and Experimental Health Sciences, vol. 13, no. 3, pp. 555–561, 2023, doi: 10.33808/clinexphealthsci.1112790.
ISNAD Çulha, Canan et al. “Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats”. Clinical and Experimental Health Sciences 13/3 (September 2023), 555-561. https://doi.org/10.33808/clinexphealthsci.1112790.
JAMA Çulha C, Gün ZÜ, Selçuk A, Apikoğlu-rabuş Ş. Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats. Clinical and Experimental Health Sciences. 2023;13:555–561.
MLA Çulha, Canan et al. “Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats”. Clinical and Experimental Health Sciences, vol. 13, no. 3, 2023, pp. 555-61, doi:10.33808/clinexphealthsci.1112790.
Vancouver Çulha C, Gün ZÜ, Selçuk A, Apikoğlu-rabuş Ş. Assessment of the Effects of Quince Seed Mucilage and Wheat Germ Oil on Wound Healing in Rats. Clinical and Experimental Health Sciences. 2023;13(3):555-61.

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