Research Article
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Year 2022, , 587 - 593, 28.09.2022
https://doi.org/10.33808/clinexphealthsci.943255

Abstract

References

  • Chevion M, Berenshtein E, Stadtman ER. Human studies related to protein oxidation: protein carbonyl content as a marker of damage. Free Radical Research 2000;33:99-108.
  • Ferrier DR. Mikrobesinler: Minareller. Lippincott Görsel Anlatımlı Çalışma Kitapları Biyokimya. 2019.p.399 (Turkish)
  • Davies MJ. Reactive species formed on proteins exposed to singlet oxygen. Photochemical Photobiological Sciences 2004; 3(1): 17-25.
  • Stadtman ER, Levine RL. Protein oxidation. Annals of the New York Academy of Sciences 2000; 899 (1); 191-208.
  • Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Acta 2003; 329(1-2); 23-38.
  • Purdel NC, Margina D, Ilie M. Current methods used in the protein carbonyl assay. Annual Research Review in Biology 2014;2015-2026.
  • Requena JR, Levine RL, Stadtman ER. Recent advances in the analysis of oxidized proteins. Amino Acids 2003;25(3-4): 221- 226.
  • Büyükgüzel E. Protein oksidasyonun biyokimyasal ve moleküler mekanizması. Karaelmas Fen ve Mühendislik Dergisi, 2013;3(1):40-51 (Turkish)
  • Zhang M, Swarts SG, Yin L, Liu C, Tian Y, Cao Y, Ju S. Antioxidant properties of quercetin. Adv Exp Med Biol. 2011;701:283-289.
  • Alrawaiq NS, Abdullah A. A review of flavonoid quercetin: metabolism, bioactivity and antioxidant properties. International Journal of PharmTech Research 2014;6(3): 933-941.
  • Baytop T. Türkiye’de Bitkiler ile Tedavi, İstanbul Üniv. Yay. No. 3637, Ecz. Fak. No.40, İstabul, 1984;240-376. (Turkish)
  • Oktay M, Yildirim A, Bilaloglu V, Gülçin I. Antioxidant activity of different parts of isgin (Rheum ribes L.). Asian Journal of Chemistry 2007;19(4): 3047.
  • Andiç S, Tunçtürk Y, Ocak E, Köse S. Some chemical characteristics of edible wild Rhubarb species (Rheum ribes L.). Res J Agric Biol Sci. 2009;5: 973-977.
  • Naqishbandi AM, Josefsen K, Pedersen ME, Jäger AK. 2009. Hypoglycemic activity of Iraqi Rheum ribes root extract. Pharmaceutical Biology 2009;47(5): 380-383.
  • Eddouks M, Maghrani M, Zeggwagh NA, Michel JB. Study of the hypoglycaemic activity of Lepidium sativum L. Aqueous extract in normal and diabetic rats, J Ethnopharmacology 2005;97: 391–395.
  • Xia E, Rao G, Van Remmen H, Heydari AR, Richardson A. Activities of antioxidant enzymes in various tissues of male Fischer 344 rats are altered by food restriction. The Journal of Nutrition 1994;125(2): 195-201.
  • Reznick AZ, Packer L. Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods in Enzymology 1994;233: 357-363.
  • Slater TF. Activation of carbon tetrachloride: Chemical principle and biological significance. Free radicals, Lipid Peroxidation and Cancer 1982;243-270.
  • Abraham P, Wilfred G. Oxidative damage to the lipids and proteins pf the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clinica Chimica Acta; International Journal of Clinical Chemistry 1999;289(1-2): 177-179.
  • Ruprah M, Mant TGK, Flanagan RJ. Acute carbon tetrachloride poisoning in 19 patients: implications for diagnosis and treatment. The Lancet 1985;325(8436):1027-1029.
  • Chen H, Tappel AL. Protection of multiple antioxidants against heme protein oxidation and lipid peroxidation induced by CBrCl3 in liver, lung, kidney, heart, and spleen. Journal of Agricultural and Food Chemistry 1996;44(3): 854-858.
  • Tirkey N, Pilkhwal S, Kuhad A, Chopra K. Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC Pharmacology 2005;5(1): 2.
  • Mehmetçik G, Özdemirler G, Koçak-Toker N, Çevikbaş U, Uysal M. Effect of pretreatment with artichoke extract on carbon tetrachloride-induced liver injury and oxidative stress. Experimental and Toxicologic Pathology 2008;60(6): 475-480.
  • Stadtman ER, Berlett BS. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metabolism Reviews 1998;30(2): 225-243.
  • Levine RL. Carbonyl assay for determination of oxidatively modified proteins. Methods. Enzymol. 1994;233: 246-257.
  • Bhadauria M, Nirala SK, Shukla S. Propolis protects CYP 2E1 enzymatic activity and oxidative stress induced by carbon tetrachloride. Molecular and Cellular Biochemistry 2007;302(1-2): 215-224.
  • Lee CP, Shih PH, Hsu CL, Yen GC. Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl4-induced oxidative damage in rats. Food and hemical Toxicology 2007;45(6): 888-895.
  • Gooneratne SR, Christensen DA. Effect of chelating agents on the excretion of copper, zinc and iron in the bile and urine of sheep. The Veterinary Journal 1997;153(2): 171-178.
  • Andersen SM. Vitamins and Minerals in The Traditional Greenland Diet. NERI Technical Report 2005;528.
  • Kuo YM, Gybina AA, Pyatskowit JW, Gitschier J, Prohaska JR. Copper transport protein (Ctr1) levels in mice are tissue specific and dependent on copper status. The Journal of Nutrition 2006;136 (1): 21-26.
  • Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Lundby C. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle. Blood 2007;109(11): 4724-4731.
  • Kasnak C, Palamutoğlu R. Doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri. Turkish Journal of Agriculture-Food Science and Technology 2015;3(5): 226-234. (Turkish)
  • Tran L, Batech M, Rhee CM, Streja E, Kalantar-Zadeh K, Jacobsen SJ, Sim JJ. Serum phosphorus and association with anemia among a large diverse population with and without chronic kidney disease. Nephrology Dialysis Transplantation 2015;31(4): 636-645.
  • Pope AM, Rall DP. Environmental medicine: İntegrating a missing element into medical education.1995
  • Sen S, Chakraborty R, Sridhar C, Reddy YSR, De B. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. International Journal of Pharmaceutical Sciences Review and Research 2010;3(1): 91-100.
  • İlhan N, Seçkin D. Protective effect of Nigella sativa seeds on CCl4-induced hepatotoxicity. FÜ Sağlık Bil Dergisi, 2005;19(3): 175-179.
  • Bayram I, Özbek H, Uğraş S, Tuncer I, Reçber D. Askorbik asit ve alfatokoferol’ün karbon tetraklorürle oluşturulmuş akut karaciğer toksisitesi modelinde karaciğeri koruyucu etkisi. Van Tıp Dergisi 2004; 11: 32-38 (Turkish).
  • Şahin A, Yener Z, Dağoğlu G. Karbon tetraklorür (CCl4) ile deneysel olarak karaciğer nekrozu oluşturulan ratlarda Vitamin E + Selenyum ve Nigella sativa’nın karaciğer yıkımını engelleyici etkileri. Turkish Journal of Veterinary and Animal Sciences 2003; 27: 141-152. (Turkish).
  • Klebanoff SJ. Myeloperoxidase: Friend and foe. J Leukoc Biol 2005; 77(5): 598 – 625.
  • Sundari PN, Wilfred G, Ramakrishna B. Does oxidative protein damage play a role in the pathogenesis of carbon tetrachloride-induced liver injury in the rat? Biochim Biopyis Acta 1997; 1362: 169-176.
  • Kılıçgün H, Altıner D. Karbontetraklorür ile karaciğer hasarı oluşturulmuş sıçanlarda Rosa canina’nın (kuşburnu) in vivo antioksidan Etkisi. Fen Bilimleri Dergisi, 2009;30(2): 10-16 (Turkish)

The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats

Year 2022, , 587 - 593, 28.09.2022
https://doi.org/10.33808/clinexphealthsci.943255

Abstract

Objective: This study was designed to examine the potential protective effects of Rheum ribes L., and quercetin on protein carbonyl (PCO) in kidney and liver tissue, trace elements (Fe, Cu, Zn) and mineral (P) in serum samples in Wistar rats of carbon tetrachloride (CCl4)-induced oxidative damage.
Methods: The 2, 4-dinitrophenylhydrazine (DNPH) method is the most reliable method widely used to measure carbonyl levels in proteins. In this study, the effect of Rheum ribes L. (Rr) and quercetin on protein carbonyl, trace elements (Fe, Cu, Zn) and mineral (P) levels against carbon tetrachloride (CCl4) mediated liver and kidney damage was investigated. For this purpose, 56 Wistar albino female rats weighing 200 ± 220 g were used. Groups were designed as: controls, 0.3 ml DMSO, 1 ml/kg olive oil, 1 ml/kg CCl4, 100 mg/kg Rr, 100 mg/kg quercetin, 100 mg/kg Rr+1 ml/kg CCl4 and 100 mg/kg quercetin+1 ml/kg CCl4 groups.
Results: The results showed that the CCl4 group had significantly higher level of protein carbonyl (PCO) than the control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.05, p<0.001, p<0.01, and p<0.01, respectively). A significant elevation in the group of CCl4 + quercetin, compare to control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.05, p<0.001, p<0.01, and p<0.05, respectively) in the liver tissue. Additionally, the CCl4 group had significantly higher level of PCO than the control, DMSO, olive oil, Rr and quercetin groups (p<0.001, p<0.01, p<0.001, p<0.001, and p<0.001, respectively). Similarly, the CCl4 + Quercetin group had increased level of PCO compared to the control and Rr.groups (p<0.05 and p<0.05) in the kidney tissue.
Conclusion: In the study, it was seen that the bioactive substances in Rheum ribes L. (root) and quercetin, a standard antioxidant, could be an alternative against the toxic effect of CCl4.

References

  • Chevion M, Berenshtein E, Stadtman ER. Human studies related to protein oxidation: protein carbonyl content as a marker of damage. Free Radical Research 2000;33:99-108.
  • Ferrier DR. Mikrobesinler: Minareller. Lippincott Görsel Anlatımlı Çalışma Kitapları Biyokimya. 2019.p.399 (Turkish)
  • Davies MJ. Reactive species formed on proteins exposed to singlet oxygen. Photochemical Photobiological Sciences 2004; 3(1): 17-25.
  • Stadtman ER, Levine RL. Protein oxidation. Annals of the New York Academy of Sciences 2000; 899 (1); 191-208.
  • Dalle-Donne I, Rossi R, Giustarini D, Milzani A, Colombo R. Protein carbonyl groups as biomarkers of oxidative stress. Clinica Chimica Acta 2003; 329(1-2); 23-38.
  • Purdel NC, Margina D, Ilie M. Current methods used in the protein carbonyl assay. Annual Research Review in Biology 2014;2015-2026.
  • Requena JR, Levine RL, Stadtman ER. Recent advances in the analysis of oxidized proteins. Amino Acids 2003;25(3-4): 221- 226.
  • Büyükgüzel E. Protein oksidasyonun biyokimyasal ve moleküler mekanizması. Karaelmas Fen ve Mühendislik Dergisi, 2013;3(1):40-51 (Turkish)
  • Zhang M, Swarts SG, Yin L, Liu C, Tian Y, Cao Y, Ju S. Antioxidant properties of quercetin. Adv Exp Med Biol. 2011;701:283-289.
  • Alrawaiq NS, Abdullah A. A review of flavonoid quercetin: metabolism, bioactivity and antioxidant properties. International Journal of PharmTech Research 2014;6(3): 933-941.
  • Baytop T. Türkiye’de Bitkiler ile Tedavi, İstanbul Üniv. Yay. No. 3637, Ecz. Fak. No.40, İstabul, 1984;240-376. (Turkish)
  • Oktay M, Yildirim A, Bilaloglu V, Gülçin I. Antioxidant activity of different parts of isgin (Rheum ribes L.). Asian Journal of Chemistry 2007;19(4): 3047.
  • Andiç S, Tunçtürk Y, Ocak E, Köse S. Some chemical characteristics of edible wild Rhubarb species (Rheum ribes L.). Res J Agric Biol Sci. 2009;5: 973-977.
  • Naqishbandi AM, Josefsen K, Pedersen ME, Jäger AK. 2009. Hypoglycemic activity of Iraqi Rheum ribes root extract. Pharmaceutical Biology 2009;47(5): 380-383.
  • Eddouks M, Maghrani M, Zeggwagh NA, Michel JB. Study of the hypoglycaemic activity of Lepidium sativum L. Aqueous extract in normal and diabetic rats, J Ethnopharmacology 2005;97: 391–395.
  • Xia E, Rao G, Van Remmen H, Heydari AR, Richardson A. Activities of antioxidant enzymes in various tissues of male Fischer 344 rats are altered by food restriction. The Journal of Nutrition 1994;125(2): 195-201.
  • Reznick AZ, Packer L. Oxidative damage to proteins: Spectrophotometric method for carbonyl assay. Methods in Enzymology 1994;233: 357-363.
  • Slater TF. Activation of carbon tetrachloride: Chemical principle and biological significance. Free radicals, Lipid Peroxidation and Cancer 1982;243-270.
  • Abraham P, Wilfred G. Oxidative damage to the lipids and proteins pf the lungs, testis and kidney of rats during carbon tetrachloride intoxication. Clinica Chimica Acta; International Journal of Clinical Chemistry 1999;289(1-2): 177-179.
  • Ruprah M, Mant TGK, Flanagan RJ. Acute carbon tetrachloride poisoning in 19 patients: implications for diagnosis and treatment. The Lancet 1985;325(8436):1027-1029.
  • Chen H, Tappel AL. Protection of multiple antioxidants against heme protein oxidation and lipid peroxidation induced by CBrCl3 in liver, lung, kidney, heart, and spleen. Journal of Agricultural and Food Chemistry 1996;44(3): 854-858.
  • Tirkey N, Pilkhwal S, Kuhad A, Chopra K. Hesperidin, a citrus bioflavonoid, decreases the oxidative stress produced by carbon tetrachloride in rat liver and kidney. BMC Pharmacology 2005;5(1): 2.
  • Mehmetçik G, Özdemirler G, Koçak-Toker N, Çevikbaş U, Uysal M. Effect of pretreatment with artichoke extract on carbon tetrachloride-induced liver injury and oxidative stress. Experimental and Toxicologic Pathology 2008;60(6): 475-480.
  • Stadtman ER, Berlett BS. Reactive oxygen-mediated protein oxidation in aging and disease. Drug Metabolism Reviews 1998;30(2): 225-243.
  • Levine RL. Carbonyl assay for determination of oxidatively modified proteins. Methods. Enzymol. 1994;233: 246-257.
  • Bhadauria M, Nirala SK, Shukla S. Propolis protects CYP 2E1 enzymatic activity and oxidative stress induced by carbon tetrachloride. Molecular and Cellular Biochemistry 2007;302(1-2): 215-224.
  • Lee CP, Shih PH, Hsu CL, Yen GC. Hepatoprotection of tea seed oil (Camellia oleifera Abel.) against CCl4-induced oxidative damage in rats. Food and hemical Toxicology 2007;45(6): 888-895.
  • Gooneratne SR, Christensen DA. Effect of chelating agents on the excretion of copper, zinc and iron in the bile and urine of sheep. The Veterinary Journal 1997;153(2): 171-178.
  • Andersen SM. Vitamins and Minerals in The Traditional Greenland Diet. NERI Technical Report 2005;528.
  • Kuo YM, Gybina AA, Pyatskowit JW, Gitschier J, Prohaska JR. Copper transport protein (Ctr1) levels in mice are tissue specific and dependent on copper status. The Journal of Nutrition 2006;136 (1): 21-26.
  • Robach P, Cairo G, Gelfi C, Bernuzzi F, Pilegaard H, Vigano A, Lundby C. Strong iron demand during hypoxia-induced erythropoiesis is associated with down-regulation of iron-related proteins and myoglobin in human skeletal muscle. Blood 2007;109(11): 4724-4731.
  • Kasnak C, Palamutoğlu R. Doğal antioksidanların sınıflandırılması ve insan sağlığına etkileri. Turkish Journal of Agriculture-Food Science and Technology 2015;3(5): 226-234. (Turkish)
  • Tran L, Batech M, Rhee CM, Streja E, Kalantar-Zadeh K, Jacobsen SJ, Sim JJ. Serum phosphorus and association with anemia among a large diverse population with and without chronic kidney disease. Nephrology Dialysis Transplantation 2015;31(4): 636-645.
  • Pope AM, Rall DP. Environmental medicine: İntegrating a missing element into medical education.1995
  • Sen S, Chakraborty R, Sridhar C, Reddy YSR, De B. Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect. International Journal of Pharmaceutical Sciences Review and Research 2010;3(1): 91-100.
  • İlhan N, Seçkin D. Protective effect of Nigella sativa seeds on CCl4-induced hepatotoxicity. FÜ Sağlık Bil Dergisi, 2005;19(3): 175-179.
  • Bayram I, Özbek H, Uğraş S, Tuncer I, Reçber D. Askorbik asit ve alfatokoferol’ün karbon tetraklorürle oluşturulmuş akut karaciğer toksisitesi modelinde karaciğeri koruyucu etkisi. Van Tıp Dergisi 2004; 11: 32-38 (Turkish).
  • Şahin A, Yener Z, Dağoğlu G. Karbon tetraklorür (CCl4) ile deneysel olarak karaciğer nekrozu oluşturulan ratlarda Vitamin E + Selenyum ve Nigella sativa’nın karaciğer yıkımını engelleyici etkileri. Turkish Journal of Veterinary and Animal Sciences 2003; 27: 141-152. (Turkish).
  • Klebanoff SJ. Myeloperoxidase: Friend and foe. J Leukoc Biol 2005; 77(5): 598 – 625.
  • Sundari PN, Wilfred G, Ramakrishna B. Does oxidative protein damage play a role in the pathogenesis of carbon tetrachloride-induced liver injury in the rat? Biochim Biopyis Acta 1997; 1362: 169-176.
  • Kılıçgün H, Altıner D. Karbontetraklorür ile karaciğer hasarı oluşturulmuş sıçanlarda Rosa canina’nın (kuşburnu) in vivo antioksidan Etkisi. Fen Bilimleri Dergisi, 2009;30(2): 10-16 (Turkish)
There are 41 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Ahmet Bakır 0000-0003-0797-285X

Suat Ekin 0000-0002-6502-5028

Sevgi Yüksek 0000-0002-9342-996X

Gokhan Oto 0000-0001-7310-7800

Publication Date September 28, 2022
Submission Date May 26, 2021
Published in Issue Year 2022

Cite

APA Bakır, A., Ekin, S., Yüksek, S., Oto, G. (2022). The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats. Clinical and Experimental Health Sciences, 12(3), 587-593. https://doi.org/10.33808/clinexphealthsci.943255
AMA Bakır A, Ekin S, Yüksek S, Oto G. The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats. Clinical and Experimental Health Sciences. September 2022;12(3):587-593. doi:10.33808/clinexphealthsci.943255
Chicago Bakır, Ahmet, Suat Ekin, Sevgi Yüksek, and Gokhan Oto. “The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats”. Clinical and Experimental Health Sciences 12, no. 3 (September 2022): 587-93. https://doi.org/10.33808/clinexphealthsci.943255.
EndNote Bakır A, Ekin S, Yüksek S, Oto G (September 1, 2022) The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats. Clinical and Experimental Health Sciences 12 3 587–593.
IEEE A. Bakır, S. Ekin, S. Yüksek, and G. Oto, “The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats”, Clinical and Experimental Health Sciences, vol. 12, no. 3, pp. 587–593, 2022, doi: 10.33808/clinexphealthsci.943255.
ISNAD Bakır, Ahmet et al. “The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats”. Clinical and Experimental Health Sciences 12/3 (September 2022), 587-593. https://doi.org/10.33808/clinexphealthsci.943255.
JAMA Bakır A, Ekin S, Yüksek S, Oto G. The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats. Clinical and Experimental Health Sciences. 2022;12:587–593.
MLA Bakır, Ahmet et al. “The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats”. Clinical and Experimental Health Sciences, vol. 12, no. 3, 2022, pp. 587-93, doi:10.33808/clinexphealthsci.943255.
Vancouver Bakır A, Ekin S, Yüksek S, Oto G. The Protective Effect of Rheum Ribes L., and Quercetin on Protein Carbonyl Levels Against Carbon Tetrachloride-Induced Liver and Kidney Damage in the Rats. Clinical and Experimental Health Sciences. 2022;12(3):587-93.

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