Evaluation of tissue factor activities and sialic acid levels in D-galactose induced aging model

Year 2014, Volume: 4 Issue: 1, 5 - 9, 25.04.2014

Ünsal Veli Üstündağ , Şehkar Oktay Ebru Emekli-alturfan Ahmet Ata Alturfan Karolin Yanar Murat Mengi Tamer Cebe Seval Aydın Ufuk Çakatay

Abstract

Objective: The aims of this study are to evaluate the changes in tissue factor (TF) activities and sialic acid (SA) concentrations of D-galactose induced rat hepatic and renal tissues and compare with the naturally aged and the control groups. 

Methods: In this study Sprague-Dawley male rats (n=28, 20 weeks old) were used. The experiment and the control groups were organized as follows: 1) young male control group (n= 8, 20 weeks old). 2) naturally aged male group (n= 10) 3) D-Galactose induced ageing group (D-galactose injected (60 mg/kg/day) for 6 weeks, n= 8, 20-week-old). At the end of the treatment, the rats were sacrificed following an overnight fast, kidney and liver tissues were immediately collected for homogenization. TF activity and SA levels were measured by the methods of Quick and Warren respectively.

Results: SA levels of kidney decreased whereas TFa (TF activity) increased significantly in the D-galactose induced aging group when compared with the control and the naturally aged groups. SA levels of liver did not change significantly between the groups. TFa of liver increased significantly compared with the control group.

Conclusion: Increased TFa (TF activity) of kidney and liver and decreased SA levels of kidney in D-galactose induced aging model show that this model can be used in studies related with aging, thrombosis and haemostasis.

Key words: D-galactose, aging, tissue factor, sialic acid

Keywords

D-galactose, aging, tissue factor, sialic acid

D-galaktoz ile oluşturulmuş yaşlanma modelinde doku faktörü aktivitesinin ve sialik asit miktarının değerlendirilmesi

Abstract

Amaç: Bu çalışmada D-galaktoz ile yaşlandırılmış, doğal yaşlı ve kontrol grubu sıçanların karaciğer ve renal dokularının doku faktörü (TF) aktivitelerinin ve inflamatuvar hastalıklar için değerli bir indikatör olan sialik asit (SA) miktarlarının ölçülmesi amaçlandı. 

Yöntemler: Çalışmada yirmi haftalık 28 adet Sprague-Dawley erkek rat kullanıldı. Gruplar; 1) genç kontrol grubu (n= 8), 2) doğal yaşlı erkek grup (n= 10), 3) 6 hafta süreyle 60 mg/kg/gün i.p. D-galaktoz enjekte edilen D-galaktoz ile yaşlandırılmış grup (n= 10) şeklinde oluşturuldu. İşlemler sonunda, ratlar bir gece aç bırakılarak sakrifiye edildi ve homojenizasyon için böbrek ve karaciğer dokuları toplandı. TF aktivitesi Quick metoduna, SA miktarı ise Warren’ın metoduna göre ölçüldü. 

Bulgular: D-galaktoz grubu böbrek dokusunda SA miktarı kontrol ve doğal yaşlı gruplara oranla anlamlı derecede azalmış, TF aktivitesi ise anlamlı derecede artmıştır. Karaciğer dokusu SA miktarında gruplar arasında anlamlı bir değişiklik gözlenmemiştir. Karaciğer TF aktivitesi ise D-galaktoz grubunda kontrol grubuna göre artmıştır. 

Sonuç: D-galaktoz ile oluşturulan yaşlanma modelinde böbrek ve karaciğer dokularında meydana gelen TF aktivitesi artışı ve böbrek SA seviyelerindeki azalma D-galaktozun yaşlanma ile ilgili tromboz/hemostaz çalışmalarında kullananılabileceğini göstermektedir.

 

Anahtar Kelimeler : D-galaktoz, yaşlanma, doku faktörü, sialik asit

Keywords

D-galaktoz, yaşlanma, doku faktörü, sialik asit

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