Nesfatin-1 ameliorates sepsis-induced remote organ injury: The role of oxidant-antioxidant status and neutrophils

Year 2018, Volume: 8 Issue: 3, 175 - 181, 28.09.2018

Zarife Nigâr Özdemir-kumral Ahmet Cumhur Ali İhsan Oluk Aykut Hoşcan İdris Önem Gazi Contuk Feriha Ercan Berrak Yeğen

https://doi.org/10.5152/clinexphealthsci.2017.606

Abstract

Purpose: Protective effects of nesfatin-1 was studied in sepsis-induced injury of

remote organs.

Methods: Male rats were randomly divided as control and sepsis (cecal ligationperforation)

groups, treated with either saline or nesfatin-1 (10 μg/kg). At 16 h

following surgery, samples of brain, kidney, liver and lung tissues were removed

and myeloperoxidase (MPO) activity, glutathione (GSH), catalase (CAT), superoxide

dismutase (SOD) and malondialdehyde (MDA) levels were measured in these

tissues.

Results: In saline-treated septic rats, elevated MDA and MPO activities were

accompanied with depleted CAT, SOD and GSH levels in the brain, kidney, liver

and lung tissues, implicating extensive oxidative damage in all remote organs.

Nesfatin-1 reduced MDA levels (brain, lung) and MPO activities (brain, kidney),

and preserved antioxidant GSH (brain, lung), CAT (brain) and SOD (kidney) levels.

Severe hepatocyte degeneration, neuronal damage, glomerulotubular degeneration

and alveolar disturbance in saline-treated septic rats were replaced with regular tissue

morphologies in nesfatin-1-treated rats.

Conclusion: Nesfatin-1 alleviates oxidative damage by enhancing endogenous

antioxidant systems and inhibiting recruitment of neutrophils, suggesting that

nesfatin-1 may be have a potential therapeutic impact on the treatment of septic

shock to reduce subsequent remote organ failure.

Keywords

Sepsis, CLP, Nesfatin-1, oxidative stress

References

• Lever A, Mackenzie I: Sepsis: definition, epidemiology, and diagnosis. BMJ 2007; 335(7625):879-83.