Group B Streptococci Induce Interleukin 8 Production in Human Cervical Epithelial Cell Cultures: The Role of Capsule Polysaccharide

Year 2019, Volume: 9 Issue: 1, 49 - 52, 29.03.2019

Burak Aksu Özgür Yanılmaz

https://doi.org/10.5152/clinexphealthsci.2018.1079

Abstract

Objective: Group B streptococci (GBS) are the major cause of pneumonia, sepsis, and meningitis in neonates and adults. Epithelial invasion and

early cytokine response of female genital tract considered to be important in the pathogenesis of GBS infection. In this study, we studied the IL-8

induction in cervical epithelial cells in response to stimulus with encapsulated (COH1) and unencapsulated (COH1-13) strains of group B streptococci.

Methods: Human cervical epithelial cancer cell (HeLa) cultures were stimulated with different concentrations (106 CFU/ml and 108 CFU/ml) of two

GBS strains. E.coli LPS was used as positive control and at specified time points (4, 8 and 24 hour) cell culture supernatant samples were collected.

IL-8 level in samples was quantified by using ELISA assay.

Results: Both GBS strains caused an equal IL-8 response in HeLa cells in a time-dependent manner. In addition, cytokine levels triggered by different

bacterial concentrations were similar and comparable with LPS.

Conclusion: Our study showed that GBS induce proinflammatory IL-8 levels in cervix epithelial cells. This induction seems to be independent from

capsule polysaccharides and suggesting that other bacterial components are involved in IL-8 stimulation.

Keywords

Streptococcus Group B, bacterial capsule, epithelial cells, IL-8

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