Investigation of Gpx1 Gene Expression, Serum Gpx1 and Selenium Levels on Colorectal Cancer

Year 2019, Volume: 9 Issue: 1, 63 - 68, 29.03.2019

Elif Sinem Iplik İhsan Nalkiran Saime Turan Soykan Arikan İlhan Yaylim Arzu Ergen

https://doi.org/10.5152/clinexphealthsci.2018.1239

Abstract

Objective: Colorectal cancer is one of the first lines when examined the death cause of cancer. It is well known that family history, and racial

and ethnic background and daily life are related with colorectal cancer and its treatment options are surgery, chemotherapeutic agents, and

radiotherapy. One of the factors that play a critical role in the development of colorectal cancer is the oxidative stress. In this research, we aimed to

investigate the association of level of glutathione peroxidase 1 (GPX1) and selenium element with colon cancer.

Methods: In this study, we aimed to determine expression levels of GPX1 genes and selenium that maintain protection against oxidative stress, with

35 colon cancer patients and their normal and tumor tissues associated with clinical and prognostic aspects by using qRT-PCR and atomic absorption

methods.

Results: The results of our study showed that GPX1 gene expression were found to be statistically significantly different (two-fold greater in normal

tissue; p < 0.05) between normal and tumor tissue. Although there was a positive correlation between serum GPX1 and selenium levels and

increase in expression of GPX1 gene and serum GPX1 levels, there was no statistical significance (p>0,05).

Conclusion: Colorectal cancer, that many factors are involved in its etiology, we have found that our preliminary studies results might show the

potential role of association between GPX1 gene expressions and selenium levels. For this reason, it has been suggested that the subject should be

supported by a large-scale group of patients.

Keywords

Colorectal cancer, glutathione peroxidase 1, selenium.

References

• [1] Nakaji S, Umeda T, Shimoyama T, et al. Environmental factors affect colon carcinoma and rectal carcinoma in men and women differently. Int J Colorectal Dis 2003;18:481-86.
• [2] Saif MW, Chu E. Biology of colorectal cancer. Cancer J 2010; 16:196-201.
• [3] Niv Y, Goel A, Boland CR. JC virus and colorectal cancer: a possible trigger in the chromosomal instability pathways. Curr Opin Gastroenterol 2005; 21:85-9.
• [4] Igney FH, Krammer PH. Death and anti-death: tumour resistance to apoptosis. Nat Rev Cancer 2002; 2:277-88.
• [5] Kerr JF, Winterford CM, Harmon BV. Apoptosis: Its significance in cancer and cancer therapy. Cancer 1994; 73(8): 2013-26.
• [6] Kairemo K, Erba P, Bergström K, Pauwels E. Nanoparticles in Cancer. Curr Radiopharm 2008; 1:30-36.
• [7] Nagler R, Savulescu D, Gavish M. Cigarette smoke-induced reduction in binding of the salivary translocator protein is not mediated by free radicals. Biochimie 2016; 121: 1-4.
• [8] Zhao J, Zhang L, Li J, Wu T, Wang M, Xu G, Zhang F, Liu L, Yang J, Sun S. A novel pyrazolone-based derivative induces apoptosis in human esophageal cells via reactive oxygen species (ROS) generation and caspase-dependent mitochondria-mediated pathway. Chem Biol Interact. 2015; 231:1-9.
• [9] Yamamoto N, Onda T, Sugahara K, Nomura T, Shibahar T. Molecular biological change in oral cancer, summary of our researches. Jpn Dent Sci Rev 2015; 51:25-33.
• [10] Limón-Pacheco J, Gonsebatt ME. The role of antioxidants and antioxidant-related enzymes in protective responses to environmentally induced oxidative stress. Mutat Res 2009; 674:137-47.
• [11] Cheeseman KH, Slater TF. An introduction to free radical biochemistry. Br Med Bull 1993; 49 (3): 481-93.
• [12] Charalabopoulos KA. Serum and tissue selenium levels in gastric cancer patients and correlation with CEA Anticancer Research 2009; 29:3465-68.
• [13] Whanger PD. Selenium and its relationship to cancer: an update dagger. Br J Nutr 2004;91:11-28.
• [14] Rayman MP. Selenium in cancer prevention: a review of the evidence and mechanism of action. Proc Nutr Soc 2005; 64:527-42.
• [15] Charalabopoulos K. Serum and tissue selenium levels in gastric cancer patients and correlation with CEA. Anticancer Res 2009; 29:3465-68.
• [16] Brinkman M. Use of selenium in chemoprevention of bladder cancer. Lancet Oncol 2006; 7:766-74.
• [17] Brinkman M. Are men with low selenium levels at increased risk of prostate cancer? Eur J Cancer 2006; 42: 2463-71.
• [18] Chen YC. Is selenium a potential treatment for cancer metastasis? Nutrients 2013; 5(4):1149-68.
• [19] Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-ΔΔCT Method. Methods 2001; 25:402-8.
• [20] Roberts RA, Laskin DL, Smith CV, Robertson FM, Allen EM, Doorn JA, et al. Nitrative and oxidative stress in toxicology and disease. Toxicol Sci 2009; 112(1): 4-16.
• [21] Hoffman CE, Webster NR, Wiggins PA, Chisholm EM, Giles GR, Leveson SH. Free radical detoxifying systems in human colorectal cancer. Br J Cancer 1985; 51: 127-29.
• [22] Hasegawa Y, Takano T, Miyauchi A, Matsuzuka F, Yoshida H, Kuma K, Amino N. Decreased expression of glutathione peroxidase mRNA in thyroid anaplastic carcinoma. Cancer Lett 2002; 182: 69-74.
• [23] Malinowska K, Mik M, Dziki Ł, Dziki A, Majsterek I. Evaluation of antioxidant defense in patients with colorectal carcinoma. Pol Przegl Chir. 2015; 87(7):357-61.
• [24] Singh SV, Brunnert SR, Roberts B, Krishan A. Differential expression of glutathione S-transferase, glutathione peroxidase and glutathione reductase in normal and malignant human breast tissues. Cancer Lett 1990; 51:43-8.
• [25] Kumaraguruparan R, Subapriya R, Viswanathan P, Nagini S. Tissue lipid peroxidation and antioxidant status in patients with adenocarcinoma of the breast. Clin Chim Acta 2002; 325 (1-2):165-70.
• [26] Brigelius-Flohe R. Selenium compounds and selenoproteins in cancer. Chem Biodivers 2008; 5: 389-95.
• [27] Saedi MS, Smith CG, Frampton J, Chambers I, Harrison PR, Sunde RA. Effect of selenium status on mRNA levels for glutathione peroxidase in rat liver. Biochem Biophys Res Commun 1988; 153(2): 855-61.
• [28] Simon HU, Haj-Yehia A, Levi-Schaffer F. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis 2000; 5: 415- 18.
• [29] Rayman MP. The importance of selenium to human health. Lancet 2000; 356 (9225): 233-41.
• [30] Chang PW, Tsui SK, Liew C, Lee CC, Waye MM, Fung KP. Isolation, characterization, and chromosomal mapping of a novel cDNA clone encoding human selenium binding protein. J Cell Biochem 1997; 64:217-24.
• [31] Lener MR, Gupta S, Scott RJ, Tootsi M, Kulp M, Tammesoo ML, Viitak A, Metspalu A, Serrano-Fernández P, Kładny J, Jaworska- Bieniek K, Durda K, Muszyńska M, Sukiennicki G, Jakubowska A, Lubiński J. Can selenium levels act as a marker of colorectal cancer risk? BMC Cancer 2013;13:214.
• [32] Duffield-Lillico AJ. Baseline characteristics and the effect of selenium supplementation on cancer incidence in a randomized clinical trial: a summary report of the Nutritional Prevention of Cancer Trial. Cancer Epidemiol Biomarkers 2002; 11:630-39.
• [33] Lippman SM. Effect of selenium and vitamin E on risk of prostate cancer and other cancers: the Selenium and Vitamin E Cancer Prevention Trial (SELECT). JAMA 2009; 301:39-51.
• [34] Reid ME. Selenium supplementation and colorectal adenomas: an analysis of the nutritional prevention of cancer trial. Int J Cancer 2006; 118:1777-81.
• [35] Hughes DJ, Fedirko V, Jenab M, Schomburg L, Méplan C, Freisling H, Bueno-de-Mesquita HB, Hybsier S, Becker NP, Czuban M, Tjønneland A, Outzen M, Boutron-Ruault MC, Racine A, Bastide N, Kühn T, Kaaks R, Trichopoulos D, Trichopoulou A, Lagiou P, Panico S, Peeters PH, Weiderpass E, Skeie G, Dagrun E, Chirlaque MD, Sánchez MJ, Ardanaz E, Ljuslinder I, Wennberg M, Bradbury KE, Vineis P, Naccarati A, Palli D, Boeing H, Overvad K, Dorronsoro M, Jakszyn P, Cross AJ, Quirós JR, Stepien M, Kong SY, Duarte-Salles T, Riboli E, Hesketh JE. Selenium status is associated with colorectal cancer risk in the European prospective investigation of cancer and nutrition cohort. Int J Cancer 2015; 136(5):1149-61.
• [36] Schrauzer GN CJ. Cancer mortality correlation studies, III. Statistical association with dietary selenium intakes. Bioinorg Chem 1977; 7:23-31.
• [37] Nilsonne G. Selenite induces apoptosis in sarcomatoid malignant mesothelioma cells through oxidative stress. Free Radic Biol Med 2006; 41:874-85.
• [38] Husbeck B. Tumor-selective killing by selenite in patientmatched pairs of normal and malignant prostate cells. Prostate 2006; 66:218-25.
• [39] Olm E. Extracellular thiol-assisted selenium uptake dependent on the x(c) – cystine transporter explains the cancer specific cytotoxicity of selenite. Proc Natl Acad Sci U S A 2009; 106:11400-