In Vitro Activity of Fosfomycin on Biofilm in Community-Acquired Staphylococcus Aureus Isolates

Year 2019, Volume: 9 Issue: 3, 202 - 209, 30.09.2019

Fikriye Milletli Sezgin Mustafa Avcu Elif Sevim Ulken Tunga Babaoglu

https://doi.org/10.33808/clinexphealthsci.599855

Abstract

Objective: Staphylococcus aureus is a significant
pathogen microorganism that can lead to serious infections. In this study, we
researched the activity of biofilm formation and fosfomycin on biofilm in
community-acquired S. aureus isolates
that were drawn from human noses.

Methods: Microtitration plate
method was used to determine biofilm formation. The effect of fosfomycin on
sessile cells was studied on biofilm matrix composed around plastic beads. The
icaA, icaD, icaB, icaC, bap, eno, fnbA, fnbB, clfA, clfB, fib, ebpS, cna and
mecA genes were screened by Polymerase Chain Reactions (PCR).

Results: S. aureus was isolated from 87 samples (13.2%) out of a total 658
nasal samples. We found that 10 of these isolates (11.4%) were
methicillin-resistant S. aureus
(MRSA). A total of 86 isolates had the ability to form biofilm. The biofilm
inhibitor concentration (BIC) and minimum biofilm eradication concentration
(MBEC) of fosfomycin were determined as 8 µg/ml and 32 µg/ml, respectively. In
the molecular detection results of biofilm-related genes of these isolates,
ica-dependent genes were determined to be quite high. However, no bap gene was
observed to be positive in any of the isolates. Among the other genes, the most
frequent genes to be declared positive were eno (97.6%) and fnbA (94.1%).

Conclusion: This study
indicates that prevalence of biofilm genes in S. aureus isolates in nasal flora is high and fosfomycin is an
effective anti-biofilm agent alone. However, to increase fosfomycin’s
efficiency, there is a need for more combination studies to make it more
effective.   

Keywords

Staphylococcus aureus, biofilm, fosfomycin, biofilm-related genes, nasal colonisation

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