Antioxidant and cytotoxic activity studies of sulfur containing glycine imine derivatives MCF-7 and DLD-1 cell lines

Year 2021, Volume: 11 Issue: 1, 81 - 90, 31.03.2021

Seda Mesci Melek Gül Tuba Yıldırım

https://doi.org/10.33808/clinexphealthsci.787954

Cited By: 1

Abstract

Objective: To investigate the antioxidant and cytotoxic activities of sulfur-containing glycine imine derivatives MCF-7 (human breast
adenocarcinoma) and DLD-1 (colorectal adenocarcinoma) cell lines.

Methods: This study examined the antioxidant activities (25-200 µM) of sulfur-containing glycine imine derivatives via the DPPH, metal chelating and reduction methods. Furthermore the cytotoxic activity of MCF-7, MCF-12A (normal breast epithelial) and DLD-1, CCD-18CO (normal colon fibroblast) were examined with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) and RTCA (Real-time Cell Analysis) assays.

Results: The antioxidant assay of the metal chelating activity showed significant results (71, 77 and 40% respectively) as compared to
knowing synthetic antioxidant (trolox; 95.45, EDTA; 97.06 %). Reducing activity was found to be very low compared to the standard compounds.Compounds were shown to be moderated by DPPH (2,2-Diphenyl-1-picrylhydrazyl) activity, and the IC50 value ranged from 91
to 150. The IC50 values (100 µM) of the MTT and RTCA analyses were similar.

Conclusion: The study showed that the compounds had selective and significant antioxidant activities, and we also found that they
had cytotoxic effects on MCF-7 and DLD-1 cells.

Keywords

Sulfur, Glycine Imine, MTT, RTCA, DPPH, Metal chelating

Project Number

FMB-BAP 18-0333

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