Pharmacopoeia Researches and Antimicrobial Activity Studies on Matricaria chamomilla L.

Year 2021, Volume: 11 Issue: 4, 801 - 808, 26.10.2021

Meryem Özdemir , Sevda Süzgeç Selçuk , Emel Mataracı Kara , Berna Özbek Çelik

https://doi.org/10.33808/clinexphealthsci.940847

Cited By: 2

Abstract

ABSTRACT
Objective: The aim of this study is to compare Matricaria chamomilla samples collected from nature and supplied from pharmacies and herbalists by performing quality control analyses and investigate the antimicrobial activities of them.
Methods: These samples were examined according to Matricariae flos monograph analysis in European Pharmacopoeia. Macroscopic and microscopic examination, loss on drying and total ash experiment were performed. Essential oil was obtained from the drugs and analyzed by thin layer chromatography. Additionally, aqueous and ethanolic extracts of all samples were compared by thin layer chromatography. Antimicrobial activity was determined by microdilution method in aqueous and ethanolic extracts.
Results: According to the pharmacopoeia analysis; in macroscopic and microscopic examinations, all samples except 2 herbalist samples were found to suitable for the pharmacopoeia standards. In loss on drying, none of the samples were found to suitable for the standards except 2 herbalist samples. In total ash, all samples were suitable for the standards. While the essential oil of M. chamomilla samples collected from nature was yellow-orange colored, we obtained the blue essential oil containing chamazulene from pharmacy samples and 3 of herbalist samples. The highest activity in antimicrobial activity assay was observed in ethanol extracts against Staphylococcus aureus, Staphylococcus epidermidis and Candida tropicalis strains.
Conclusion: Examples of true M. chamomilla were found among herbalist samples, contrary to the previous studies. Pharmacy samples were qualified but limited in terms of variety. Essential oil analyses pointed out the importance of geographical location among natural samples. Antimicrobial activity tests did not provide significant results.

Keywords

Matricaria chamomilla, chamomile, pharmacopoeia analysis, antimicrobial activity

References

• Baytop T. Therapy with Medicinal Plants in Turkey. 2nd ed. Istanbul: Nobel Medical Publishing; 1999.
• Blumenthal M, Goldberg A, Brinckmann J. Herbal Medicine: Expanded Commission E Monographs. Newton, Massachusetts: Integrative Medicine Communications; 2000.
• Bisset NG. Herbal Drugs and Phytopharmaceuticals. Stuttgart: Medpharm Scientific Publishers; 1994.
• Gruenwald J, Brendler T, Jaenicek C. PDR for Herbal Medicines. 2nd ed. Montvale, New Jersey: Medical Economics Company; 2000.
• Barnes J, Anderson LA, Phillipson JD. Herbal Medicines. 3rd ed. Chicago: Pharmaceutical Press; 2007.
• Roby MHH, Sarhan MA, Selim KAH, Khalel KI. Antioxidant and antimicrobial activities of essential oil and extracts of fennel (Foeniculum vulgare L.) and chamomile (Matricaria chamomilla L.). Ind Crop Prod 2013; 44:437-445.
• Göger G, Demirci B, Ilgın S, Demirci F. Antimicrobial and toxicity profiles evaluation of the chamomile (Matricaria recutita L.) essential oil combination with standard antimicrobial agents. Ind Crop Prod 2018; 120:279-285.
• Flemming M, Kraus B, Rascle A, Jürgenliemk G, Fuchs S, Fürst R, Heilmann J. Revisited anti-inflammatory activity of matricine in vitro: Comparison with chamazulene. Fitoterapia 2015; 106:122-128.
• Cvetanović A, Švarc-Gajić J, Zeković Z, Jerković J, Zengin G, Gašić U, Tešić Ž, Mašković P, Soares C, Barroso MF, Delerue-Matos C, Đurović S. The influence of the extraction temperature on polyphenolic profiles and bioactivity of chamomile (Matricaria chamomilla L.) subcritical water extracts. Food Chem 2019; 271:328-337.
• Nayak BS, Raju SS, Rao AV. Wound healing activity of Matricaria recutita L. extract. J Wound Care 2007; 16(7):298-302.
• Sampaio TPD, Holmes TSV, De Medeiros Nóbrega DR, Dos Santos MGC, Bento PM, De Castro Gomes DQ, Pereira JV. Matricaria recutita Linn. in the prevention and control of radio-induced oral mucositis. Oral Surg Oral Med Oral Pathol Oral Radiol 2014; 117(2):e215.
• Jabri MA, Aissani N, Tounsi H, Sakly M, Marzouki L, Sebai H. Protective effect of chamomile (Matricaria recutita L.) decoction extract against alcohol-induced injury in rat gastric mucosa. Pathophysiology 2017; 24(1):1-8.
• Yazdi H, Seifi A, Changizi S, Khori V, Hossini F, Davarian A, Jand Y, Enayati A, Mazandarani M, Nanvabashi F. Hydro-alcoholic extract of Matricaria recutita exhibited dual anti-spasmodic effect via modulation of Ca2+ channels, NO and PKA2-kinase pathway in rabbit jejunum. Avicenna J Phytomed 2017; 7(4):334-344.
• Hajaji S, Sifaoui I, López-Arencibia A, Reyes-Batlle M, Jiménez IA, Bazzocchi IL, Valladares B, Akkarari H, Lorenzo-Morales J, Piñero JE. Leishmanicidal activity of α-bisabolol from Tunisian chamomile essential oil. ParasitoL Res 2018; 117(9):2855-2867.
• Prasanna R, Ashraf EA, Essam MA. Chamomile and oregano extracts synergistically exhibit antihyperglycemic, antihyperlipidemic, and renal protective effects in alloxan-induced diabetic rats. Can J Physiol Pharmacol 2017; 95(1):84-92.
• Ionita R, Postu PA, Mihasan M, Gorgan DL, Hancianu M, Cioanca O, Hritcu L. Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study. Phytomedicine 2018; 47:113-120.
• Chandrashekhar VM, Halagali KS, Nidavani RB, Shalavadi MH, Biradar BS, Biswas D, Muchchandi IS. Anti-allergic activity of German chamomile (Matricaria recutita L.) in mast cell mediated allergy model. J Ethnopharmacol 2011; 137(1):336-340.
• Zargaran A, Borhani-Haghighi A, Salehi-Marzijarani M, Faridi P, Daneshamouz S, Azadi A, Sadeghpour H, Sakhteman A, Mohagheghzadeh A. Evaluation of the effect of topical chamomile (Matricaria chamomilla L.) oleogel as pain relief in migraine without aura: A randomized, double-blind, placebo-controlled, crossover study. Neurol Sci 2018; 39(8):1345-1353.
• Abdoul-Latif FM, Mohamed N, Edou P, Ali AA, Djama SO, Obame LC, Bassole HN, Dicko MH. Antimicrobial and antioxidant activities of essential oil and methanol extract of Matricaria chamomilla L. from Djibouti. J Med Plant Res 2011; 5(9):512-1517.
• Mekonnen A, Yitayew B, Tesema A, Taddese S. In vitro antimicrobial activity of essential oil of Thymus schimperi, Matricaria chamomilla, Eucalyptus globulus, and Rosmarinus officinalis. Int J Microbiol 2016; 2016.
• İlkimen H, Gülbandılar A. Lavanta, ada çayı, kekik ve papatya ekstrelerinin antimikrobiyal etkilerinin araştırılması. Turk Microbiol Cem Derg 2018; 48(4):241-246.
• Tolouee M, Alinezhad S, Saberi R, Eslamifar A, Zad SJ, Jaimand K, Taeb J, Rezaee MB, Kawachi M, Shams-Ghahfarokhi M. Effect of Matricaria chamomilla L. flower essential oil on the growth and ultrastructure of Aspergillus niger van Tieghem. Int J Food Microbiol 2010; 139(3):127-133.
• Kazemi M. Chemical composition and antimicrobial activity of essential oil of Matricaria recutita. Int J Food Prop 2015; 17(11).
• Jamalian A, Shams-Ghahfarokhi M, Jaimand K, Pashootan N, Amani A, Razzaghi-Abyaneh M. Chemical composition and antifungal activity of Matricaria recutita flower essential oil against medically important dermatophytes and soil-borne pathogens. J Mycol Med 2012; 22(4):308-315.
• Rahman H, Chandra A. Microbiologic evaluation of Matricaria and chlorhexidine against E. faecalis and C. albicans. Indian J Dent 2015; 6(2):60.
• Vora J, Srivastava A, Modi H. Antibacterial and antioxidant strategies for acne treatment through plant extracts. Inform Med Unlocked 2018; 13:128-132.
• Cvetanović A, Švarc-Gajić J, Mašković P, Savić S, Nikolić L. Antioxidant and biological activity of chamomile extracts obtained by different techniques: perspective of using superheated water for isolation of biologically active compounds. Ind Crop Prod 2015; 65:582-591.
• Munir N, Iqbal AS, Altaf I, Bashir R, Sharif N, Saleem F, Naz S. Evaluation of antioxidant and antimicrobial potential of two endangered plant species Atropa belladonna and Matricaria chamomilla. Afr J Tradit Complement Altern Med 2014; 11(5):111-117.
• Serpi M, Ozdemir ZO, Salman Y. Investigation of the antibacterial effects of some plant extracts on Propionibacterium acnes. KSU J Agric Nat 2012; 15(1):7-12.
• Jafarzadeh MM, Moghaddam MJM, Bakhshi D. Antimicrobial activity of three plant species against multi-drug resistant E. coli causing urinary tract infection. J Herb Med 2020; 22:100352.
• Guzelmeric E, Vovk I, Yesilada E. Development and validation of an HPTLC method for apigenin 7-O-glucoside in chamomile flowers and its application for fingerprint discrimination of chamomile-like materials. J Pharm Biomed Anal 2015; 107:108-118.
• European Pharmacopoeia 7th ed. Council of Europe, Strasbourg, France; 2011.
• Baytop A. Bitkisel Drogların Anatomik Yapısı. İstanbul: İstanbul Üniversitesi Eczacılık Fakültesi Yayınları; 1975.
• Abdelhameed RM, Abdel-Gawad H, Taha M, Hegazi B. Separation of bioactive chamazulene from chamomile extract using metal-organic framework. J Pharm Biomed Anal 2017; 146:126-134.
• Mericli AH. The lipophilic compounds of a Turkish Matricaria chamomilla variety with no chamazulene in the volatile oil. Pharm Biol 1990; 28(2):145-147.