In Vitro Assessment of Anti-inflammatory Effect of Apigenin on Renal Cell Inflammation

Year 2022, Volume: 12 Issue: 3, 739 - 745, 28.09.2022

Selen Özsoy , Gül Fatma Yarım

https://doi.org/10.33808/clinexphealthsci.1018335

Abstract

Objective: This study aimed to evaluate in vitro effect of apigenin on anti – and pro-inflammatory cytokines including interleukin-6 (IL-6), IL-10, tumor necrosis factor-alpha (TNF-α), and transforming growth factor-beta (TGF-β) levels in an in vitro model of renal cell inflammation induced with lipopolysaccharide (LPS).

Methods: For the in vitro renal cell inflammation model, the African green monkey kidney cell line (Vero) was used. Four groups as NC (without any treatment), LPS (Vero cells treated with 10 μg/mL of LPS for 4 hours), API (Vero cells treated with 5 μg/mL of apigenin for 12 hours), and LPS+API (Vero cells treated with 5 μg/mL of apigenin for 12 hours + 10 μg/mL of LPS for 4 hours) was formed. The non-cytotoxic dose of apigenin in Vero cells was evaluated by a cell count test. IL-6, IL-10, TNF-α, and TGF-β concentrations in the cell culture medium were measured by enzyme-linked immunosorbent assay kits. All analyses were performed in four repetitions.

Results: IL-6, IL-10, TNF-α, and TGF-β concentrations of the LPS group increased compared to NC, API, and LPS+API groups (p<0.05). We found that treatment with apigenin led to significant attenuation in the LPS-induced secretion of IL-6, IL-10, TNF-α, and TGF-β in the Vero cell line.

Conclusion: Our findings showed that apigenin significantly reduced LPS-induced IL-6, IL-10, TNF-α, and TGF-β formations in Vero cells. Taken together, these results suggest that apigenin may be a therapeutic candidate for relieving inflammatory renal cell damage. These results need to be supported by in vivo trials and clinical applications.

Keywords

Apigenin, in vitro, lipopolysaccharide, renal cell damage

Supporting Institution

Ondokuz Mayis University

Project Number

PYO.VET.1904.17.014

Thanks

This study was supported by the Scientific Project Management Office of the Ondokuz Mayis University, Samsun, Turkey (PYO.VET.1904.17.014).

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