Synthesis of Nanoparticles Loading Indenopyrazole Derivatives and Evaluation of Biological Features

Year 2022, Volume 12, Issue 4, 830 - 834, 26.10.2022

Murat DOĞAN Ümit Muhammet KOÇYİĞİT Hayreddin GEZEGEN

https://doi.org/10.33808/clinexphealthsci.1055921

Abstract

Objective: In this study, it was aimed to prepare nanoparticle formulations using chitosan, a cationic natural polymer, and tripoly phosphate, and to perform mechanical characterization and in vitro cell culture studies. In addition, the cytotoxic effects of nanoparticles containing indenopyrazol derivatives against human glioma cells (C6) and human cervical cancer cells (HeLa) were investigated. Methods: Within the scope of the study, nanoparticles containing indenopyrazole derivative were prepared and characterization of particle size, zeta potential and morphological properties were performed. XTT cytotoxicity test was applied to evaluate the antiproliferative activities of nanoparticles containing these components. Results: Particle size, zeta potential and morphological properties of nanoparticles were observed to be suitable for application. In vitro cell culture studies showed that nanoparticles containing indenopyrazol derivatives showed better cytotoxic effects in both cell lines. Conclusion: The results showed that the mechanical properties of nanoparticles containing indenopyrazol derivatives are suitable and can be applied in anticancer activity studies.

Keywords

Chitosan, Indenopyrazole derivatives, Nanoparticle, Antiproliferative activity

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