Synthesis of Nanoparticles Loading Indenopyrazole Derivatives and Evaluation of Biological Features

Year 2022, Volume 12, Issue 4, 830 - 834, 30.12.2022

Murat DOĞAN Ümit Muhammet KOÇYİĞİT Hayreddin GEZEGEN

https://doi.org/10.33808/clinexphealthsci.1055921

Abstract

Objective: In this study, it was aimed to prepare nanoparticle formulations using chitosan, a cationic natural polymer, and tripoly phosphate, and to perform mechanical characterization and in vitro cell culture studies. In addition, the cytotoxic effects of nanoparticles containing indenopyrazol derivatives against human glioma cells (C6) and human cervical cancer cells (HeLa) were investigated. Methods: Within the scope of the study, nanoparticles containing indenopyrazole derivative were prepared and characterization of particle size, zeta potential and morphological properties were performed. XTT cytotoxicity test was applied to evaluate the antiproliferative activities of nanoparticles containing these components. Results: Particle size, zeta potential and morphological properties of nanoparticles were observed to be suitable for application. In vitro cell culture studies showed that nanoparticles containing indenopyrazol derivatives showed better cytotoxic effects in both cell lines. Conclusion: The results showed that the mechanical properties of nanoparticles containing indenopyrazol derivatives are suitable and can be applied in anticancer activity studies.

Keywords

Chitosan, Indenopyrazole derivatives, Nanoparticle, Antiproliferative activity

References

• Kumar J. Ashoket. Synthesis, anticancer activity and photophysical properties of novel substituted 2-oxo-2Hchromenylpyrazolecarboxylates. European Journal of Medicinal Chemistry 2013; 65: 389-402.
• Kamel MM, El-Ansary AK and Milad YR. Design, synthesis, and cytotoxicity of pyridine, pyrazole, and thiazole derivatives derived from N-alkyl-4, 5, 6, 7-tetrahydro-1-benzothiophene. Chemistry of Heterocyclic Compounds 2013; 49(3): 392-403.
• Mor S, Nagoria S, Kumar A, Monga J, Lohan S. Convenient synthesis, anticancer evaluation and QSAR studies of some thiazole tethered indenopyrazoles. Medicinal Chemistry Research 2016; 25(6): 1096-1114.
• Tutone M, and Almerico A. Recent advances on CDK inhibitors: An insight by means of in silico methods. European Journal of Medicinal Chemistry 2017; 142: 300-315.
• Singh S, Sharma B, Kanwar SS, Kumar A, Lead Phytochemicals for Anticancer Drug Development. Front. Plant Sci. 2016; 7: 8973-8985.
• Gezegen H, Tutar U, Hepokur C, Ceylan M. Synthesis and biological evaluation of novel indenopyrazole derivatives. J Biochem Mol Toxicol. 2019; 33: e22285.
• Hamedi H, Moradi S, Hudson SM, Tonelli AE. Chitosan based hydrogels and their applications for drug delivery in wound dressings: A review. Carbohydr Polym. 2018; 199: 445-460.
• Chikara S, Nagaprashantha LD, Singhal J, Horne D, Awasthi S, Singhal SS. Oxidative stress and dietary phytochemicals: Role in cancer chemoprevention and treatment. Cancer Lett. 2018; 413: 122–134.
• Pucci C, Martinelli C, Ciofani G. Innovative approaches for cancer treatment: Current perspectives and new challenges. Ecancermedicalscience 2019; 13:961.
• Michael J, Majcher H, Todd H. Functional Biopolymers, Polymers and Polymeric Composites: A Reference Series (1st ed). Applications of Hydrogels 2019; 453-491.
• Martinho N, Damgé C, Reis CP. Recent advances in drug delivery systems. J Biomater Nanobiotechnol. 2011; 2: 510-526.
• Watkins R, Wu L, Zhang C, Davis RM, Xu B. Natural productbased nano medicine: recent advances and issues. Int J Nanomed. 2015;10: 6055-6074.
• Andrews GP, Laverty TP, Jones DS. Mucoadhesive polymeric platforms for controlled drug delivery. Eur. J. Pharm. Biopharm. 2009; 71: 505-518.
• Taşkın T, Dogan M, Yilmaz BN, Senkardes I, Phytochemical screening and evaluation of antioxidant, enzyme inhibition, anti-proliferative and calcium oxalate anti-crystallization activities of Micromeria fruticosa spp. brachycalyx and Rhus coriaria. Biocatalysis and Agricultural Biotechnology 2020; 27: 1-7.
• Calvo P, Remunan-Lopez C, Vila-Jato JL, Alonso MJ. Novel hydrophilic chitosan – polyethylene oxide nanoparticles as protein carriers. J Appl Polym Sci. 1997; 63: 125-32.
• Wikanta T, Erizal T, Tjahyono T, Sugiyono T. Synthesis of polyvinyl alcohol-chitosan hydrogel and study of its swelling and antibacterial properties. Squalen 2012; 7(1):1-10.
• Gezegen H, Tutar U, Hepokur C, Ceylan M. Synthesis and biological evaluation of novel indenopyrazole derivatives. Journal of Biochemical and Molecular Toxicology. 2019; 33(5):e22285.