Nadir Yayılışlı Tanacetum nivale Sch.Bip. (Asteraceae) Üzerine Morfolojik, Palinolojik ve Mikromorfolojik İncelemeler

Abstract

Asteraceae familyasının Anthemideae tribusuna ait kozmopolit cinslerden biri olan Tanacetum L., yaklaşık 160 türü kapsamaktadır. Türkiye’de bu cinse ait 61 takson bulunmakta olup, bunların 27’si endemiktir. Bu araştırmada, Tanacetum nivale Sch.Bip.’nin morfolojik, polen ve aken özellikleri ışık mikroskobu (LM) ve taramalı elektron mikroskobu (SEM) kullanılarak incelenmiş; cins içerisindeki sistematik problemlerin çözümüne katkı sağlayabilecek ek karakterler ortaya konulmuştur. Hakkâri’deki Cilo Dağları’ndan toplanan örneklere dayanılarak türün genişletilmiş ve revize edilmiş bir tanımı sunulmuştur. Polenler izopolar, radyal simetrili ve trizonokolporat apertür tipine sahiptir. Oblat-sferoidal şekilli olup, polar eksen uzunluğu 25–30 μm, ekvatoral eksen uzunluğu ise 26–32 μm arasında değişmektedir. Ekzin ornamentasyonu ekinat yapıdadır ve dikenler arasında mikroretikülat-rugulat bir desen gözlenmiştir. Akenler saman sarısı renkli, obkonikal-oblong şekilli, 1,24–2,15 × 0,32–0,62 mm boyutlarında olup, yüzey ornamentasyonu skalariform-verrukat-striat karakter göstermektedir. Elde edilen verilerin, Tanacetum cinsine ait diğer taksonlar üzerinde yürütülecek gelecekteki çalışmalara ışık tutacağı ve morfolojik, palinolojik ile aken makro-mikromorfolojik özelliklere dayalı takson ayrımında kullanılabilecek sistematik karakterlerin belirlenmesine katkı sağlayacağı düşünülmektedir.

Keywords

• Tanacetum
• Morfoloji
• Polen
• Aken
• SEM

Supporting Institution

TÜBİTAK, proje numarası 123Z282

Project Number

123Z282

Ethical Statement

Bu araştırma kapsamında insan katılımcılar veya hayvanlar üzerinde herhangi bir çalışma yürütülmemiştir. Bu nedenle etik kurul onayı gerekmemektedir.

Thanks

Bu araştırma, Gazi Üniversitesi Biyoloji Bölümü’nde yürütülen “Tanacetum nivale Sch.Bip., Tanacetum oltense (Sosn.) Grierson ve Tanacetum punctatum (Desr.) Grierson Türleri Üzerine Morfolojik, Mikromorfolojik, Anatomik ve Palinolojik Araştırmalar” başlıklı yüksek lisans tez çalışmasının bir bölümünü oluşturmaktadır. Çalışma, TÜBİTAK tarafından 123Z282 numaralı proje kapsamında finansal olarak desteklenmiştir.

Morphological, Palynological and Micromorphological Investigations on Unusual Tanacetum nivale Sch.Bip. (Asteraceae)

Abstract

The genus Tanacetum L., is one of the cosmopolitan members of the tribe Anthemideae (Asteraceae), encompassing approximately 160 species. Within Türkiye, 61 taxa are recorded, 27 of which are endemic. This research investigates Tanacetum nivale Sch.Bip. using LM and SEM to assess its morphological, pollen, and achene traits, providing additional characters that may assist in resolving systematic issues within the genus. An expanded and revised description is presented based on specimens collected in the Cilo Mountains in Hakkari. The pollen exhibit isopolar, radial symmetry, and a trizonocolporate aperture type. They display an oblate-spheroidal shape, with a polar axis measuring 25–30 μm and an equatorial axis measuring 26–32 μm. The exine ornamentation is echinate with a microreticulate-rugulate pattern observed between the spines. The achenes are straw-colored, obconical-oblong, 1.24–2.15 × 0.32–0.62 mm, with scalariform-verrucate-striate surface ornamentation. The obtained data will shed light on future studies on other taxa of the genus Tanacetum, and contribute to systematic characters for the discrimination of taxa based on morphological, palynological, and achene macro-micromorphological features.

Keywords

• Tanacetum
• Morphology
• Pollen
• Achene
• SEM

Supporting Institution

TUBITAK

Project Number

123Z282

Ethical Statement

This work was performed without involving human participants or animal subjects. Therefore, no ethical approval was required.

Thanks

This research constitutes a section of a master’s thesis titled “Morphological, Micromorphological, Anatomical and Palynological Studies on Tanacetum nivale Sch.Bip., Tanacetum oltense (Sosn.) Grierson and Tanacetum punctatum (Desr.) Grierson” being conducted at Gazi University, Department of Biology. Financial support for this study was provided by TUBITAK, project number 123Z282.

References

1. Bremer, K., Humphries, C.J., Generic monograph of the Asteraceae–Anthemideae. Bulletin of the British Museum (Natural History) Botany, 23 (1993), 71-177.
2. Funk, V.A., Susanna, A., Stuessy, T.F., Robinson, H. Classification of Compositae. In: Funk, V.A., Susanna, A., Stuessy, T.F., Bayer, R.J. Editors. Systematics, evolution, and biogeography of Compositae. International Association for Plant Taxonomy (IAPT), Vienna, Austria, (2009), 171-189.
3. Heywood, V.H. Tanacetum. In: Tutin, T.G., Heywood, V.H., Burges, N.A., Moore, D.M., Valentine, D.H., Walters, S.M., Webb, D.A. Editors. Flora Europaea, Volume 4. Cambridge University Press, Cambridge, (1976), 169-171.
4. Kadereit, J.W., Jeffrey, C., The Families and Genera of Vascular Plants, Vol. 8: Flowering Plants, Eudicots, Asterales. Springer-Verlag: Berlin, 2007. https://doi.org/10.1007/978-3-540-31051-8
5. Grierson, A.J.C. Tanacetum L. In: Davis, P.H. Editor. Flora of Turkey and the East Aegean Islands, Volume 5. Edinburgh University Press, Edinburgh, (1975), 256-292.
6. Güler, H.S., Karyotype analyses of some Tanacetum L. (Asteraceae) taxa. Master Thesis, Karadeniz Technical University, Trabzon, Türkiye, 2010.
7. Linnaeus, C., Species Plantarum. Salvius: Stockholm, 1753.
8. Boissier, E., Flora Orientalis, Volume 3. H. Georg: Geneva, Switzerland, 1875.

9. Güner, A., Özhatay, N., Ekim, T., Başer, K.H.C., Flora of Turkey and the East Aegean Islands, supplement 2. Edinburgh University Press: Edinburgh, 2000.
10. Korkmaz, M., Kandemir, A., İlhan, V., Yıldırım Doğan, N., Tanacetum erzincanense (Asteraceae), a new species from Erzincan, Turkey. Turkish Journal of Botany, 39 (2015), 96-104. https://doi.org/10.3906/bot-1311-2
11. Kandemir, A. Tanacetum. In: Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. Editors. Checklist of the Flora of Turkey (Vascular Plants). Nezahat Gökyiğit Botanik Bahçesi Yayınları, İstanbul, 2012.
12. Abad, M.J., Bermejo, P., Villar, A., An approach to the genus Tanacetum L. (Compositae): Phytochemical and pharmacological review. Phytotherapy Research, 9 (2) (1995), 79-92. https://doi.org/10.1002/ptr.2650090202
13. Bohlmann, F., Zdero, C., Sesquiterpene lactones and other constituents from Tanacetum parthenium. Phytochemistry, 21 (10) (1982), 2543-2549. https://doi.org/10.1016/0031-9422(82)85253-9
14. Berry, M.I., Feverfew faces the future. Pharmaceutical Journal, 232 (1984), 611-614.
15. Asımgil, A., Şifalı bitkiler. Timaş Yayınları: İstanbul, 1993.
16. Gören, N., Tahtasakal, E, Sesquiterpenoids from Tanacetum argenteum subsp. canum var. canum. Phytochemistry, 45 (1997), 107-109. https://doi.org/10.1016/S0031-9422(96)00790-X
17. Newall, C.A., Anderson, L.A., Phillipson, J.D., Herbal Medicines, a Guide for Health-Care Professionals. The Pharmaceutical Press: London, 1996.
18. Baytop, T., Türkiye’de Bitkiler ile Tedavi, Geçmiste ve Bugün. Nobel Tıp Kitabevleri: İstanbul, 1999.
19. Wodehouse, R.P., Pollen grain morphology in the classification of the Anthemideae. Bulletin of the Torrey Botanical Club, 53 (1926), 479-485. https://doi.org/10.2307/2480028
20. Wodehouse, R.P., The phylogenetic value of pollen grain characters. Annals of Botany, 42 (1928), 891-934. https://doi.org/10.1093/oxfordjournals.aob.a090149
21. Wodehouse, R.P., Pollen Grains: Their Structure, Identification and Significance in Science and Medicine. McGraw-Hill: New York, 1935.
22. Erdtman, G., Berglund, B., Praglowski, J., An introduction to a scandinavian pollen flora. Grana Palynology, 2 (2) (1961), 3-92. https://doi.org/10.1080/00173136109428945
23. Punt, W., Hoen, P.P., The Northwest European pollen flora, 70: Asteraceae– Asteroideae. Review of Palaeobotany and Palynology, 157 (2009), 22-183. https://doi.org/10.1016/j.revpalbo.2008.12.003
24. Osman, A.K., Zareh, M.M. Palynological studies on Anthemideae (Asteraceae) in Egypt. In: Flora & Phytotaxonomy researches Dept. and The Egyptian Botanical Society. Proceedings of First International Conference Strategy of Egyptian Herbaria, Giga, Egypt, March 9-11, 2004.
25. Özmen, E., Kızılpınar, I., Özudoğru, B., Doğan, C., Erik, S., Pollen morphology of some taxa of aromatic genus Tanacetum L. (Asteraceae). Journal of Pharmaceutical Sciences, 34 (2009), 1-11.
26. Kodak, E., Pınar, N.M., Adıgüzel, N., Acar Şahin, A., Pollen morphology of some taxa of genus Tanacetum L. (Asteraceae) in Turkey. Mellifera, 12 (24) (2012), 2-10.
27. Kürşat, M., Çeltik, İ., Başer, B., Özbey, F., Emre, İ., Pollen morphology of some Tanacetum L. (Asteraceae) taxa and its systematic value. Turkish Journal of Botany 47 (5) (2023), 372-387. https://doi.org/10.55730/1300-008X.2775
28. Mukherjee, S.K., Sarkar, A.K., Morphology and structure of cypselas in thirteen species of the tribe Astereae (Asteraceae). Phytomorphology, 51 (2001), 17-26.
29. Abid, R., Qaiser, M., Taxonomic significance of the cypsela morphology in the tribe Anthemideae (Asteraceae) from Pakistan and Kashmir. Pakistan Journal of Botany, 41 (2009), 555-579.
30. Kothari, J., Sharma, K.C., Sharma, M., Micromorphology of fruit surfaces in some taxa of the tribe Astereae (Asteraceae) and their taxonomic significance. Indian Journal of Plant Sciences, 1 (2012), 240-262.
31. Kulkarni, S.V., SEM studies of achenes in some taxa of Asteraceae. International Journal for Environmental Rehabilitation and Conservation, 4 (2013), 87-97.
32. Hussein, H.A., Eldemerdash, M.M., Comparative morphology and surface microsculpture of cypsela in some taxa of the Asteraceae and their taxonomic significance. Egyptian Journal of Botany, 56 (2) (2017), 409-422. https://doi.org/10.21608/ejbo.2017.1133
33. Ghimire, B., Gang, U.S., Lee, C.H., Heo, K., Jeong, M.J., Cypsela morphology of Cirsium species (Asteraceae) and its systematic implications. Flora, 249 (2018), 40-52.
34. Özbek, M.U., Özbek, F., Vural, M., Achene morphology of the genus Cota J. Gay (Asteraceae) from Turkey and its taxonomic significance. Turkish Journal of Botany, 42 (2) (2018), 208-223. https://doi.org/10.3906/bot-1707-61
35. Bona, M., Systematic importance of achene macro-micromorphological characteristics in selected species of the genera Crupina, Jurinea, and Klasea (Asteraceae) from Turkey. Microscopy Research and Technique, 83 (11) (2020), 1345-1353. https://doi.org/10.1002/jemt.23526
36. Özbek, F., Morphological characteristics of pollen and cypsela in the genus Rhaponticum (Asteraceae) from Turkey and their systematic significance. Plant Biosystems, 156 (6) (2022), 1485-1497. https://doi.org/10.1080/11263504.2022.2098867
37. Kynclova, M., Comparative morphology of achenes of the tribe Anthemideae Cass. (Asteraceae) and its taxonomic significance. Preslia, 42 (1970), 33-53.
38. Bhar, I., Mukherjee, S.K., Macromorphological and micromorphological study of cypselas in seven species of the tribe Anthemideae (Asteraceae). Journal of Economic and Taxonomic Botany, 28 (2004), 788-794.
39. Kodak, E., Pollen, fruit and leaf micromorphology of some Tanacetum taxa. Doctoral Thesis, Ankara University, Ankara, Türkiye, 2013.
40. Behjou, A.M., Sonboli, A., Riahi, H., Kazempour Osaloo, S., Achene micromorphology in Tanacetum (Asteraceae–Anthemideae) and its taxonomic and phylogenetic implications. Flora, 222 (2016), 37-51. https://doi.org/10.1016/j.flora.2016.03.012
41. Erdtman, G., Handbook of Palynology: Morphology, Taxonomy and Ecology. Munksgaard: Copenhagen, 1969.
42. Fægri, K., Iversen, J., Textbook of Pollen Analysis, 3rd ed. Munksgaard: Copenhagen, 1975.
43. Punt, W., Hoen, P.P., Blackmore, S., Nilsson, S., Le Thomas, A., Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology, 143 (2007), 1-81. https://doi.org/10.1016/j.revpalbo.2006.06.008
44. Özbek, M.U., Özbek, F., Başer, B., Cabi, E., Vural, M., Pollen morphology of the genus Cota J. Gay (Asteraceae) in Turkey. Botany Letters, 163 (4) (2016), 435-448. https://doi.org/10.1080/23818107.2016.1225266
45. Barthlott, W., Epidermal and seed surface characters of plants: systematic applicability and some evolutionary aspects. Nordic Journal of Botany, 1 (1981), 345-355. https://doi.org/10.1111/j.1756-1051.1981.tb00704.x
46. Bojňanský, V., Fargašová, A., Atlas of Seeds and Fruits of Central and East-European Flora: The Carpathian Mountains Region. Springer: Dordrecht, 2007. https://doi.org/10.1007/978-1-4020-5362-7
47. Erdtman, G., Pollen Morphology and Plant Taxonomy-Angiosperms. Almqvist & Wiksell: Stockholm, 1952.
48. Stix, E., Pollen morphologische untersuchungen an compositen. Grana Palynologica, 2 (1960), 41-114. https://doi.org/10.1080/00173136009429443
49. Skvarla, J.J., Turner, B.L., Patel, V.C., Tobb, A.S. Pollen morphology in the Compositae and in morphologically related families. In: Heywood, V.H., Harborne, J.B., Turner, B.L. Editors. The biology and chemistry of the Compositae. Academic Press, London, (1977), 141-248.
50. Mesfin, T., Crawford, D.J., Smith, E.B., Pollen morphology of North Coreopsis (Compositae). Grana, 34 (1) (1995), 21-27. https://doi.org/10.1080/00173139509429029
51. Çeter, T., Pınar, N.M., İnceer, H., Hayırlıoğlu Ayaz, S., Yaprak, A.E., The comparative pollen morphology of genera Matricaria L. and Tripleurospemum Sch. Bip. (Asteraceae) in Turkey. Plant Systematics and Evolution, 299 (2013), 959-977. https://doi.org/10.1007/s00606-013-0776-z
52. Lo Presti, R.M., Oppolzer, S., Oberprieler, C.H., A molecular phylogeny and a revised classification of the Mediterranean genus Anthemis s.l. (Compositae, Anthemideae) based on three molecular markers and micromorphological characters. Taxon, 59 (2010), 1441-1456. https://doi.org/10.1002/tax.595010