PUTRESCINE AS A PROTECTIVE MOLECULE ON DNA DAMAGE AND DNA METHYLATION CHANGES IN WHEAT UNDER DROUGHT

Abstract

The world suffers with the agricultural drought stress which leading to decreasing crop production, and also adversely affecting cereals on morphological, physiological, biochemical and molecular levels. However, exogenous treatment of some osmotically active materials like putrescine has been regarded as a good preventive against these harmful effects of drought. But there is a lack of information on putrescine has any effects on DNA damage and DNA methylation in crops. The current study was goal to determine DNA damage levels and DNA methylation changes in Triticum aestivum cv. Karasu 90 subjected to different concentrations of drought (-2, -4, -6 bar PEG) and whether putrescine (0.01, 0.1, 1 mM) has any ameliorative effect on these changes is determined with RAPDs and CRED-RAs tecniques. In addition, total oxidant status (TOS) and total antioxidant status (TAS) values were investigated based on drought and putrescine treatments. The findings showed that drought stress caused DNA damage and DNA methylation changes. However, these effects decreased after putrescine treatments. Putrescine has been shown to decrease oxidative damage caused by drought via increasing antioxidant status in drought stress. According to results, it was concluded that putrescine could be preferred for its force to protect wheat DNA from the damaging effects of drought and the demethylation positively contributed to drought stress tolerance.

Keywords

• CRED-RA,DNA Methylation,Genomic Template Stability,RAPD,Total oxidant status,Total antioxidant status

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