COBALT AND ASCORBIC ACID TREATMENT: EFFECTS ON TISSUE NITRIC OXIDE LEVELS IN STREPTOZOTOCIN- DIABETIC RAT

Abstract

The aim of present study was to determine the effect of cobalt (II) chloride (CoCl2) and CoCl2plus ascorbic acid (AA) on liver and kidney nitric oxide level at different stage of diabetes. Three days after streptozotocin (STZ) injection, rats were divided into six groups: control, diabetic, control and diabetic treated with cobalt chloride, control and diabetics treated with cobalt chloride plus ascorbic acid. 0.5 mM CoCl2 or 0.5 mM CoCl2 plus 1 g/l ascorbic acid were added to their drinking water daily. At the end of the second, fourth and sixth week of the treatment all animals were sacrified by decapitation. Liver and kidney tissues were removed for the determination of nitric oxide (NO) levels. Cobalt treatment effectively decreased the nitric oxide levels both in liver and kidney tissues of diabetic rats. Thus, the effect of oral administration of cobalt treatment may prove effective in improving the impaired nitrite status during the early stage of diabetes and ascorbic acid supplementation at this dose (1g/l) potentiates the effectiveness of cobalt action

Keywords

• Nitric oxide,diabetes,cobalt,ascorbic acid,liver and kidney

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