TEMPERATURE AND THE LIFE CYCLE OF HETERODERA SCHACHTII (NEMATODA: HETERODERIDAE)

Abstract

Development of the sugar beet cyst nematode, Heterodera schachtii, was studied in growth chambers at 20, 25, 29, 32 and 35 ± 1 ºC on Beta vulgaris cv. Saccharifera. The optimum temperature for reproduction appeared to be 32 ºC, at which the life cycle, from second-stage juvenile (J2) to J2, was completed in 16-19 days; at 35 ºC, 20-21 days were required. Juveniles emerged from eggs within 29 days at 29 ºC and after 43 days at 25 ºC. Although J2 were present within eggs after 62 days at 20 ºC, emergence was not observed up to 101 days after inoculation. Female nematodes produced fewer eggs at 20 ºC than at higher temperatures

Keywords

• Sugar beet cyst nematode,Heterodera schachtii,life cycle,temperature

References

1. Asbach, K., Cai, D., Jung, C.H., Pauselius, I., 2004. Investigation on the interaction between beet cyst nematode and Arabidopsis thaliana carrying resistance from sugar beet. Plant Nematology, 2: 256-270.
2. Bajaj, H.K., Gupta, D.C., Dahiya, R.S., 1986. Development of Heterodera zeae on wheat and maize. Nematologica, 32: 209-215.
3. Baltensperger, D.B., Kerr, E., Nielsen, E., 2000. Evaluating relative suitability of rapeseed vs. sugarbeet as a host for Heterodera schachtii. Web/URL: http://www.sbreb.org/research/ento/ento99/99p220.htm.
4. Byrd, D.W., Kirkpatrick, T., Barker, K.R., 1983. An improved techniquie for clearing and staining plant tissues for detection of nematodes. Journal of Nematology, 15: 142-143.
5. De Greef, W., Jacobs, M., 1979. In vitro culture of sugar beet: Description of a cell line with high regeneration capacity. Plant Sci. Lett., 17: 55-61.
6. Ferris, P.A.R., Williamson, V.M., 2004. Host plant resistance (HPR) against nematodes. Biol. Approaches to manage. California, 14: 21-30.
7. Franklin, M.T., 1977. Heterodera schachtii. In: C.I.H. Description of plant parasitic nematodes. Eds. S. Willmott, P.S. Gooch, M.R. Siddiqi and M.T. Franklin. Commonwealth Agricultural Bureaux, Famham Royal Slough, U.K., Set: 1, No.1.
8. Gamborg, O.L., Miller, R.A., Ojima, K., 1976. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res., 50: 151-158.

9. Paul, H., Deelen, J.E.M., Henken, B., Bock, S.M., Lang, W., Krens, F.A., 1990. Expression in vitro of resistance to Heterodera schachtii in hairy roots of an alien monotelosomic addition plant of Beta vulgaris, transformed by Agrobacterium rhizogenes. Euphytica, 48: 153-157.
10. Shadmehr, A., Nouroozi, P., Garousi, G., Ahmedi, A.R., 2007. Study of sugar beet cyst nematode life cycle using plant tissue culture method. Pakistan Journal of Biological Science, 10(17): 2910-2914.
11. Southey, J.F., 1970. Laboratory methods for work with plant and soil nematodes. Ministry of Agriculture, Fisheries Food. London. pp:72.
12. Srivastava, A.N., Sethi, C.L., 1985. On the larval penetration and biology of Heterodera zeae on maize. Indian Journal of Nematology, 15: 18-20.