INVESTIGATION ON ANTIMICROBIAL EFFECTS OF SOME MOSS SPECIES COLLECTED FROM KASTAMONU REGION

Abstract

It has been known for centuries that mosses are effective in wound healing process. Previous studies presented that some secondary metabolites of mosses have anti-infective effect on some microorganisms. In this study, in vitro antimicrobial effect of Bryum capillare var. capillare, Plagiomnium affine, Homalothecium sericeum, Homalia besseri, Amblystegium tenax and Homalothecium lutescens samples collected from Kastamonu region were examined against Candida albicans ATCC 26555, Salmonella enterica Serotype Typhium SL 1344, Escherichia coli ETEC LM 63083, Shigella flexneri and Bacillus megaterium. The antimicrobial activities of moss extracts were evaluated by disc diffusion method and the results were supported MIC (minimum inhibitory concentration) and MBC/MFC (minimum bactericidal/fungicidal concentration) tests. According to the results, among the moss samples, Bryum capillare var. capillare presented an antimicrobial activity against S. flexneri and B. megaterium, where Homalothecium sericeum and Amblystegium tenax had an antimicrobial activity against only S. flexneri, and Homalia besseri against only B. megaterium

Keywords

• Mosses, antimicrobial activity, disc diffusion method, MIC, MBC, MFC

References

1. Altuner EM (2008). Bazı karayosunu türlerinin antimikrobiyal aktivitesinin belirlenmesi, Ph. D., Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara.
2. Altuner EM, Çetin B (2009). Antimicrobial activity of Thuidium delicatulum (Bryopsida) extracts. Kafkas Üniversitesi, Fen Bilimleri Enstitüsü Dergisi. 2: 85-92.
3. Altuner EM, Çetin B (2010). Antimicrobial activity of Tortella tortulosa (Hedw.) Limpr. extracts, Kastamonu University Journal of Forestry Faculty, 10(2): 111-116.
4. Altuner EM, Çetin B, Çökmüş C. (2010a). Antimicrobial Screening of Some Mosses Collected From Anatolia, Pharmacognosy Magazine, 6 (22): 56.
5. Altuner EM, Çetin B, Çökmüş C (2010b). Isotechium alopecuroides Türünün Parametresinin Etkisi, Ecology 2010 Symposium Proceedings, p.96. Aktivitesi Üzerine Lokasyon
6. Andrews JM (2007). BSAC standardized disc susceptibility testing method (version 6). Journal of Antimicrobial Chemotherapy, 60:20-41.
7. Basile A, Vuotto ML, Ielpo TL, Moscatiello V, Ricciardi L, Giordano S, Cobianchi RC (1998). Antibacterial activity in Rhynchostegium riparoides (Hedw.) Card. Extract (Bryophyta). Phytotherapy Research, 12: 146 - 148.
8. Cowan MM (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 564-582.

9. Erdogrul ÖT (2002). Antibacterial Activities of Some Plant Extracts Used in Folk Medicine. Pharmaceutical Biology, 40:4, 269-273.
10. Geissman TA (1963). Flavonoid compounds, tannins, lignins and related compounds, p. 265. In M. Florkin and E. H. Stotz (ed.), Pyrrole pigments, isoprenoid compounds and phenolic plant constituents, vol. 9. Elsevier, New York.
11. Hammer KA, Carson CF, Riley TV (1999). Antimicrobial activity of essential oils and other plant extracts. Journal of Applied Microbiology, 86: 985-990.
12. Lis-Balchin M, Deans SG (1997). Bioactivity of selected plant essential oils against Listeria monocytogenes. Journal of Applied Bacteriology, 82: 759-762.
13. Mahasneh AM, El-Oqlah AA (1999). Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan. J Ethnopharmacol, 64: 271-276.
14. Reynolds JEF (1996). Martindale - the Extra Pharmacopoeia, thirty first ed. Royal Pharmaceutical Society of Great Britain, London.
15. Samidurai K, Saravanakuma A (2009). Antibacterial Activity of Pemphis Acidula Forst. Global Journal of Pharmacology, 3(2):113-115.
16. Saroya AS (2011). Herbalism, Phytochemistry and Ethnopharmacology. Herbal Consultant, Punjab, India.
17. Silici S, Koc AN (2006). Comparative study of in vitro methods to analyse the antifungal activity of propolis against yeasts isolated from patients with superficial mycoses. Letters in Applied Microbiology, 43:318-324.
18. Veljic M, Tarbuk M, Marin PD, Ciric A, Sokovic M, Marin M (2008). Antimicrobial Activity of Methanol Extracts of Mosses from Serbia. Pharmaceutical Biology, 46:12, 871-875.