INVESTIGATION OF THE ANTIMICROBIAL ACTIVITY OF GAGEA

Abstract

The main objective of this study is to evaluate the antimicrobial effect of ethanol solution extracts of G. dubia A. Terracc. on nineteen gram positive and negative bacteria (Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Enterobacter aerogenes, Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Salmonella infantis, Salmonella typhimurium, Salmonella kentucky, Salmonella enterica, Pseudomonas aeruginosa, Pseudomonas fluorescens) and one fungi (Candida albicans). Different parts (flowers, leaves, stems, and roots) of Gagea dubia samples, were extracted with 80% ethanol solution. These extracts were tested in vitro for their antimicrobial activity against 15 microorganism by disk diffusion (DD) and minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests. Ethanol extracts showed antimicrobial activity against some bacteria. According to the results of disk diffusion test G. dubia have effect on four bacteria (B. subtilis, S. aureus, L. innocua and E. durans) and (C. albicans) and didn’t show effect on other microorganisms. The antibacterial effect observed in 10 µL extract of G.dubia against B. subtilis, S. aureus, L.innocua with 7,66; 13 and 9 mm zone diameter respectively and 50 µL extract of G. dubia against E.durans with 11 mm zone diameter. G. dubia extract show antimicrobial activity at three concentration (10;50 and 100 µL) against C. albicans between 915,66 mm zone diameter. MIC result for G. dubia determined as 5; 1,25; 10; 5 and 0,15 mg/100 µL against B. subtilis, S. aureus, L.innocua, E.durans and C. albicans respectively. According to MBC test all MIC value against maicroorganism determined as bacteriostatic

Keywords

• Gagea dubia,Liliaceae,Antimicrobial effect,MIC,MBC,Disk diffusion

References

1. [1] A.A. Abdalla, C.Y. Ishak and S.M.H Ayoub, Antimicrobial activity of four medicinal plants used by Sudanese traditional medicine. Journal of Forest Products and Industries, 2, (2013) 29-33.
2. [2] M.A. Weiner, Earth medicine-earth food: plant remedies, drugs, and natural foods of the North American Indians. Macmillan. (1980).
3. [3] D.E. Moerman, An analysis of the food plants and drug plants of native North America. Journal of Ethnopharmacology, 52(1), (1996) 1-22.
4. [4] B. Klink, Alternative medicines: is natural really better. Drug Top, 141(2), (1997) 99-100.
5. [5] M.W. Iwu, A.R. Duncan, and C.O. Okunji, New antimicrobials of plant origin. Perspectives on new crops and new uses. ASHS Press, Alexandria, VA, (1999) 457-462.
6. [6] C.U. Anyanwu and G.C Nwosu, . Assessment of the anticrobial acts of piler guineense leaves. Journal of Medicinal Plant Research, 8(10), (2014) 436- 440.
7. [7] B.B. Petrovska, Historical review of medicinal plants’usage. Pharmacognosy Reviews, 6(11), (2012) 1-5
8. [8] N. Stojanoski, Development of health culture in Veles and its region from the past to the end of the 20th century. Veles Society of Science and Art, (1999) 13-34.

9. [9] R.P. Borris, Natural products research: perspectives from a major pharmaceutical company. Journal of Ethnopharmacology, 51(1-3), (1996) 29-38.
10. [10] A.T. Sheldon Jr, Antibiotic resistance: a survival strategy. Clinical Laboratory Science, 18 (3), (2005) 170-180.
11. [11] M.S. Ali-Shtayeh, A.A. Al-Assali and R.M. Jamous, Antimicrobial activity of Palestinian medicinal plants against acne-inducing bacteria. African Journal of Microbiology Research, 7(21), (2013) 2560-2573.
12. [12] E.M. Altuner, T. Ceter, E. Bayar, S. Aydın, F. Arıcı, G. Suleymanoglu and A. Edis, Investigation on Antimicrobial Effects of Some Moss Species Collected From Kastamonu Region, Commun. Fac. Sci. Univ. Ank. Series C: Biology, 23(1-2), (2011) 33-43.
13. [13] E.M. Altuner, T. Ceter, D. Demirkapı, K. Ozkay, U. Hayal, G.Eser, Investigation on Antimicrobial Effects of Some Lichen Species Collected From Kastamonu Region, Communication Faculty Science University Ankara Series C: Biology, 23(1-2), (2011) 21-31.
14. [14] J.M. Andrews, BSAC standardized disc susceptibility testing method (version 6). Journal of Antimicrobial Chemotherapy, 60, (2007) 20-41.
15. [15] E.M. Altuner, T. Ceter and C. Islek, Cilt enfeksiyonlarının geleneksel tedavisinde kullanılan Ononis spinosa L. kulunun antifungal etkisinin araştırılması. Mikrobiyoloji Bülteni, 44(4), (2010) 633-639.