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Year 2019, Volume: 28 Issue: 1, 91 - 100, 30.06.2019

Abstract

References

  • U. M. Zanger and M. Schwab, Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation, Pharmacology & Therapeutics, 138 (1), (2013) 103-141.
  • K. Barabas, R. Milner, D. Lurie, and C. Adin, Cisplatin: a review of toxicities and therapeutic applications, Veterinary and Comparative Oncology, 6 (1), (2008) 1-18.
  • R.P. Miller, R.K. Tadagavadi, G. Ramesh and W.B. Reeves, Mechanisms of cisplatin nephrotoxicity, Toxins, 2 (11), (2010) 2490-2518.
  • R. Mosenden and K. Tasken, Cyclic AMP-mediated immune regulation—overview of mechanisms of action in T cells, Cellular Signalling, 23 (6), (2011) 1009-1016.
  • G. Akbal, Sisplatin verilmiş ratlarda cAMP ve CoQ’nun karaciğer radikal süpürücü enzim aktiviteleri üzerine etkilerinin ve hasar giderilmesindeki rollerinin araştırılması, Doktora Tezi, Ondokuz Mayıs Universitesi, Fen Bilimleri Enstitüsü, (2010).
  • F.J. Gonzalez and H.V. Gelboin, Role of human cytochromes P450 in the metabolic activation of chemical carcinogens and toxins, Drug Metabolism Reviews, 26 (1-2), (1994) 165-183.
  • V.P. Androutsopoulos, A.M. Tsatsakis and D.A. Spandidos, Cytochrome P450 CYP1A1: wider roles in cancer progression and prevention, BMC Cancer, 9 (1), (2009) 187.187.
  • H. Liu, L. Shi, J.P. Giesy and H. Yu, Polychlorinated diphenyl sulfides can induce ROS and genotoxicity via the AhR-CYP1A1 pathway, Chemosphere, 223, (2019) 165-170.
  • S. Winslow, A. Scholz, P. Rappl, T.F. Brauß, C. Mertens, M. Jung and T. Schmid, Macrophages attenuate the transcription of CYP1A1 in breast tumor cells and enhance their proliferation, Plos One, 14 (1), (2019) e0209694.
  • J. P. Wu, L. W. Chang, H. T. Yao, H. Chang, H. T. Tsai, M. H.Tsai and P. Lin, Involvement of oxidative stress and activation of aryl hydrocarbon receptor in elevation of CYP1A1 expression and activity in lung cells and tissues by arsenic: an in vitro and in vivo study, Toxicological sciences, 107 (2), (2008) 385-393.
  • L. Han, L. Li, G.J. Muehlbauer, J.E. Fowler and M M. Evans, RNA Isolation and analysis of LncRNAs from gametophytes of maize, In Plant Long Non-Coding RNAs (pp. 67-86), Humana Press, New York, (2019).
  • F.J. Gonzalez, The molecular biology of cytochrome P450s, Pharmacological Reviews, 40 (4), (1988) 243-288.
  • X. Ding and L.S. Kaminsky, Human extrahepatic cytochromes P450: function in xenobiotic metabolism and tissue-selective chemical toxicity in the respiratory and gastrointestinal tracts, Annual Review of Pharmacology and Toxicology, 43 (1), (2003) 149-173.
  • S.A.Wrighton, M.VandenBranden and B.J. Ring, The human drug metabolizing cytochromes P450, Journal of Pharmacokinetics and Biopharmaceutics, 24 (5), (1996) 461-473.
  • M.E. McManus, I. Stupans, B. Ioannoni, W. Burgess, R.A. Robson and D.J. Birkett, Identification and quantitation in human liver of cytochromes P-450 analogous to rabbit cytochromes P-450 forms 4 and 6, Xenobiotica, 18 (2), (1988) 207-216.
  • D.J. Adams, S. Seilman, Z. Amelizad, F. Oesch and C.R. Wolf, Identification of human cytochromes P-450 analogous to forms induced by phenobarbital and 3-methylcholanthrene in the rat, Biochemical Journal, 232 (3), (1985) 869-876.
  • S.A. Wrighton, C. Campanile, P.E. Thomas, S.L. Maines, P.B. Watkins, G. Parker and W. Levin et al., Identification of a human liver cytochrome P-450 homologous to the major isosafrole-inducible cytochrome P-450 in the rat. Molecular Pharmacology, 29 (4), (1986) 405-410.
  • H. Schweikl, J.A. Taylor, S. Kitareewan, P. Linko, D. Nagorney and J.A. Goldstein, Expression of CYP1A1 and CYP1A2 genes in human liver. Pharmacogenetics, 3 (5), (1993) 239-249.
  • F.P. Guengerich, Mechanisms of cytochrome P450 substrate oxidation: MiniReview, Journal of Biochemical and Molecular Toxicology, 21(4), (2007) 163-168.

COMPARISON OF TOXIC AND ANTITOXIC EFFECTS OF CISPLATIN AND CAMP, AT CYP1A1 GENE EXPRESSION LEVEL OF WISTAR ALBINO TYPE RAT LIVER

Year 2019, Volume: 28 Issue: 1, 91 - 100, 30.06.2019

Abstract

Throughout the course of cancer treatment, cisplatin has proven to be a
successful chemotherapy agent. Several researches have been conducted in vivo and/or in
vitro on gene expression changes occurring with the impact of this agent. The current study
focuses on the alterations in CYP1A1 gene expression levels in liver cells by cisplatin and
cAMP treatment. CYP1A1 gene which the expression levels were analyzed, encodes a
member of the cytochrome P450 superfamily of enzymes. Throughout the study, liver
tissues from Wistar-type albino rat, also known as the laboratory rat, were used. For
normalization of the data, the housekeeping gene TATA box binding protein (TBP) was
preferred. The results showed that; gene expression was reduced in tissues subjected
independently to cisplatin and cAMP, however the tissues treated with cAMP showed
further reduction in gene expression levels. In contrast, tissues subjected to both cisplatin
and cAMP showed an increase in the level of expression. This study aims to provide an
alternative perspective for comparative toxicology cases and will also serve as a guide for
elucidation of cases connected with forensic toxicology.
  

References

  • U. M. Zanger and M. Schwab, Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation, Pharmacology & Therapeutics, 138 (1), (2013) 103-141.
  • K. Barabas, R. Milner, D. Lurie, and C. Adin, Cisplatin: a review of toxicities and therapeutic applications, Veterinary and Comparative Oncology, 6 (1), (2008) 1-18.
  • R.P. Miller, R.K. Tadagavadi, G. Ramesh and W.B. Reeves, Mechanisms of cisplatin nephrotoxicity, Toxins, 2 (11), (2010) 2490-2518.
  • R. Mosenden and K. Tasken, Cyclic AMP-mediated immune regulation—overview of mechanisms of action in T cells, Cellular Signalling, 23 (6), (2011) 1009-1016.
  • G. Akbal, Sisplatin verilmiş ratlarda cAMP ve CoQ’nun karaciğer radikal süpürücü enzim aktiviteleri üzerine etkilerinin ve hasar giderilmesindeki rollerinin araştırılması, Doktora Tezi, Ondokuz Mayıs Universitesi, Fen Bilimleri Enstitüsü, (2010).
  • F.J. Gonzalez and H.V. Gelboin, Role of human cytochromes P450 in the metabolic activation of chemical carcinogens and toxins, Drug Metabolism Reviews, 26 (1-2), (1994) 165-183.
  • V.P. Androutsopoulos, A.M. Tsatsakis and D.A. Spandidos, Cytochrome P450 CYP1A1: wider roles in cancer progression and prevention, BMC Cancer, 9 (1), (2009) 187.187.
  • H. Liu, L. Shi, J.P. Giesy and H. Yu, Polychlorinated diphenyl sulfides can induce ROS and genotoxicity via the AhR-CYP1A1 pathway, Chemosphere, 223, (2019) 165-170.
  • S. Winslow, A. Scholz, P. Rappl, T.F. Brauß, C. Mertens, M. Jung and T. Schmid, Macrophages attenuate the transcription of CYP1A1 in breast tumor cells and enhance their proliferation, Plos One, 14 (1), (2019) e0209694.
  • J. P. Wu, L. W. Chang, H. T. Yao, H. Chang, H. T. Tsai, M. H.Tsai and P. Lin, Involvement of oxidative stress and activation of aryl hydrocarbon receptor in elevation of CYP1A1 expression and activity in lung cells and tissues by arsenic: an in vitro and in vivo study, Toxicological sciences, 107 (2), (2008) 385-393.
  • L. Han, L. Li, G.J. Muehlbauer, J.E. Fowler and M M. Evans, RNA Isolation and analysis of LncRNAs from gametophytes of maize, In Plant Long Non-Coding RNAs (pp. 67-86), Humana Press, New York, (2019).
  • F.J. Gonzalez, The molecular biology of cytochrome P450s, Pharmacological Reviews, 40 (4), (1988) 243-288.
  • X. Ding and L.S. Kaminsky, Human extrahepatic cytochromes P450: function in xenobiotic metabolism and tissue-selective chemical toxicity in the respiratory and gastrointestinal tracts, Annual Review of Pharmacology and Toxicology, 43 (1), (2003) 149-173.
  • S.A.Wrighton, M.VandenBranden and B.J. Ring, The human drug metabolizing cytochromes P450, Journal of Pharmacokinetics and Biopharmaceutics, 24 (5), (1996) 461-473.
  • M.E. McManus, I. Stupans, B. Ioannoni, W. Burgess, R.A. Robson and D.J. Birkett, Identification and quantitation in human liver of cytochromes P-450 analogous to rabbit cytochromes P-450 forms 4 and 6, Xenobiotica, 18 (2), (1988) 207-216.
  • D.J. Adams, S. Seilman, Z. Amelizad, F. Oesch and C.R. Wolf, Identification of human cytochromes P-450 analogous to forms induced by phenobarbital and 3-methylcholanthrene in the rat, Biochemical Journal, 232 (3), (1985) 869-876.
  • S.A. Wrighton, C. Campanile, P.E. Thomas, S.L. Maines, P.B. Watkins, G. Parker and W. Levin et al., Identification of a human liver cytochrome P-450 homologous to the major isosafrole-inducible cytochrome P-450 in the rat. Molecular Pharmacology, 29 (4), (1986) 405-410.
  • H. Schweikl, J.A. Taylor, S. Kitareewan, P. Linko, D. Nagorney and J.A. Goldstein, Expression of CYP1A1 and CYP1A2 genes in human liver. Pharmacogenetics, 3 (5), (1993) 239-249.
  • F.P. Guengerich, Mechanisms of cytochrome P450 substrate oxidation: MiniReview, Journal of Biochemical and Molecular Toxicology, 21(4), (2007) 163-168.
There are 19 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Review Articles
Authors

Kumbirai Deon Mandebere This is me 0000-0002-8234-1444

F. Seyma Gokdemır 0000-0003-2951-848X

Gonul Solmaz 0000-0002-9400-5173

Sumer Aras This is me 0000-0003-3474-9493

Publication Date June 30, 2019
Acceptance Date May 20, 2019
Published in Issue Year 2019 Volume: 28 Issue: 1

Cite

Communications Faculty of Sciences University of Ankara Series C-Biology.

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