ISOLATION OF ENTOMOPATHOGENIC FUNGI FROM TURKEY SOIL AND TESTING OF DIFFERENT DOSES ON GALLERIA MELLONELLA (LEPIDOPTERA: PYRALIDAE)

Year 2020, Volume: 29 Issue: 2, 213 - 242, 31.12.2020

Yusuf Doğan , Nevin Keskin

Abstract

Isolation of biological control agents which would be used with pests control could be done with different methods. The most sensitive of these methods is the insect bait method. Besides, it is known that the effects of different entomopathogen fungi isolates, which would be used as biological control agents varies in different hosts. In this study, since the target pest organism is the larval period of Galleria mellonella Linnaeus (Lepidoptera: Pyralidae), this pest is used for insect bait method and as a result of isolation from 180 soil samples, 48 Beauveria bassiana Bals.-Criv. Vuill. (Hypocreales: Cordycipitaceae) and 1 Beauveria brongniartii (Sacc.) Petch (Hypocreales: Cordycipitaceae) was detected but Metarhizium spp. wasn’t detected. Furthermore, it is known that the precise determination of the entomopathogen fungi isolates, plays a crucial role in the success of a biological control program. In this study, as biological control agent, four concentrations (1x104, 1x105, 1x106 and 1x107 conidiya/ml) of 10 different isolates of Beauveria bassiana Bals.-Criv. Vuill. (Hypocreales: Cordycipitaceae), which were obtained from soils of our country and a standard strain from Denmark were applied to Galleria mellonella Linnaeus (Lepidoptera: Pyralidae) larvae. When Lethal times (LT50) compared, it was found that the highest concentration of each isolates was also the most effective one.

Keywords

Biological control, Entomoğathogen fungi, Insect bait method, Beauveria bassiana, Galleria mellonella

Supporting Institution

Hacettepe Üniversitesi Fen Bilimleri Enstitüsü Biyoloji Bölümü

Thanks

We thank to Nicolai V. Meyling, who shared the type species sample which is KVL 03-129 (ARSEF 8032) with us for use in our study and Prof. Dr. Alev UZTAN, who assisted in the diagnosis of entomopathogen fungi isolated from the soil.

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