Determination of chemical content of Lemna minor L. by GC-MS and investigation of antioxidant activity
Year 2022,
Volume: 31 Issue: 1, 53 - 64, 30.06.2022
Sema Yiyit Doğan
,
Sibel Atasagun
,
Mehmet Borga Ergönül
Abstract
Lemna minor L. has been traditionally used for a long time for its analgesic, antipyretic, vitamin C supplement, astringent, antipruritic effects. Although there are many heavy metal removals using L. minor, unfortunately, biological activity studies are very limited. In this study, the chemical content and total phenol content, DPPH removal, metal chelation (Fe2+) and β-Carotene-lycopene methods of the L. minor macrophyte we obtained from Turkey were determined by GC-MS. The results of the study showed that our plant contains 25 different essential oils and has a high phenol content. In addition, 72% DPPH removal of L.minor was determined when it had 71% iron chelating ability. As a result of our study, it has been revealed that the L. minor we use is an effective antioxidant. It is thought that its usability in the fields of food and medicine can be investigated with further studies.
Supporting Institution
Coordination of Scientific Research Projects of Ankara University
Project Number
21L0430004
Thanks
This work was financially supported by the Coordination of Scientific Research Projects of Ankara University (Research Project No: 21L0430004) in Turkey. We would like to thank Asst. Prof. Ayşegül H. MENDİ for her assistance.
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Year 2022,
Volume: 31 Issue: 1, 53 - 64, 30.06.2022
Sema Yiyit Doğan
,
Sibel Atasagun
,
Mehmet Borga Ergönül
Project Number
21L0430004
References
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- Preci, D. P., Almeida, A., Weiler, A. L., Franciosi, M. L. M., Cardoso, A. M., Oxidative damage and antioxidants in cervical cancer, International Journal of Gynecologic Cancer, 31(2) (2021). http://dx.doi.org/10.1136/ijgc-2020-001587
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- González-Renteria, M., del Carmen Monroy-Dosta, M., Guzmán-García, X., Hernández-Calderas, I., Antibacterial activity of Lemna minor extracts against Pseudomonas fluorescens and safety evaluation in a zebrafish model, Saudi Journal of Biological Sciences, 27(12) (2020), 3465-3473. https://doi.org/10.1016/j.sjbs.2020.09.043
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- Yağcıoğlu, P., Farklı ekstraksiyon metotları ile adaçayı (Salvia officinalis L.) bitkisinden antioksidan ekstraksiyonunun optimizasyonu (Doctoral dissertation, Fen Bilimleri Enstitüsü), 2015.
- Barros, L., Ferreira, M. J., Queiros, B., Ferreira, I. C., Baptista, P., Total phenols, ascorbic acid, β-carotene and lycopene in Portuguese wild edible mushrooms and their antioxidant activities, Food chemistry, 103(2) (2007), 413-419. https://doi.org/10.1016/j.foodchem.2006.07.038
- Blois, M. S., Antioxidant determinations by the use of a stable free radical, Nature, 181(4617) (1958), 1199-1200. https://doi.org/10.1038/1811199a0
- Decker, E. A., & Welch, B., Role of ferritin as a lipid oxidation catalyst in muscle food, Journal of Agricultural and food Chemistry, 38(3) (1990), 674-677.
- Nagata, M., Yamashita, I., Simple method for simultaneous determination of chlorophyll and carotenoids in tomato fruit, Nippon Shokuhin Kogyo Gakkaishi, 39(10) (1992), 925-928. https://doi.org/10.3136/nskkk1962.39.925
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- Moraes, J., de Oliveira, R. N., Costa, J. P., Junior, A. L., de Sousa, D. P., Freitas, R. M., & Pinto, P. L., Phytol, a diterpene alcohol from chlorophyll, as a drug against neglected tropical disease Schistosomiasis mansoni, PLoS Neglected Tropical Diseases, 8(1) (2014), e2617. https://doi.org/10.1371/journal.pntd.00026
Varsha, K. K., Devendra, L., Shilpa, G., Priya, S., Pandey, A., Nampoothiri, K. M., 2, 4-Di-tert-butyl phenol as the antifungal, antioxidant bioactive purified from a newly isolated Lactococcus sp., International journal of food microbiology, 211 (2015), 44-50. https://doi.org/10.1016/j.ijfoodmicro.2015.06.025
- Petrova-Tacheva, V., Ivanov, V., & Atanasov, A., Lemna minor L. as a source of antioxidants, Trakia Journal of Science, 18 (2020), 157-162. http://doi 10.15547/tjs.2020.s.01.029
- Kim, Y., Hyun, S. H., Park, H. E., & Choi, H. K., Metabolic profiling, free-radical scavenging and tyrosinase inhibitory activities of Lemna minor whole plants cultivated in various concentrations of proline and sucrose, Process Biochemistry, 47(1) (2012), 62-68. https://doi.org/10.1016/j.procbio.2011.10.010
- Tran, H. C., Le, H. A. T., Le, T. T., Effects of Enzyme Types and Extraction Conditions on Protein Recovery and Antioxidant Properties of Hydrolysed Proteins Derived from Defatted Lemna minor, Applied Science and Engineering Progress, 14(3) (2021), 360-369. https://doi.org/10.14416/j.asep.2021.05.003
- Saritha, K., Saraswathi, U., Antioxidant activity of gold nanoparticles synthesized using Lemna minor, World Journal of Pharmaceutical Sciences, (2014), 1545-1551.
- Iskandar, I., Andriani, Y., Rostika, R., Zidni, I., & Riyanti, N. A., Effect of using fermented Lemna sp. in fish feed on growth rate of nilem carp (Osteochilus hasselti), World News of Natural Sciences, 26 (2019), 157-166.
- Baynal, Ö. F., Likopen, Erciyes Üniversitesi Eczacılık Fakültesi, Bitirme tezi, 2012.