Embryonic development of the lemon-yellow tree frog, $Hyla~ savignyi$ Audouin, 1827

Year 2023, Volume: 32 Issue: 2, 192 - 207, 30.12.2023

Şefik Karanlık , Elnaz Najafı-majd , Elif Yıldırım , Uğur Kaya

https://doi.org/10.53447/communc.1350057

Abstract

Amphibians are widely used in temperature adaptation studies due to their compatibility in laboratory experiments. We investigated the embryonic development stages (from fertilization to $25^{th}$) of $Hyla ~savignyi$ following Gosner’s generalized table. Three pairs of $H.~ savignyi$ were collected during the breeding season (February 2015) from Northern Cyprus, Kalkanlı Region and maintained at 21±1 °C in the laboratory. The samples were set in 3 groups and examinations of embryos and photographs taken every 10 minutes were carried out during the 9-days embryonic period. Embryos hatched at stage 20 or 21 come up to 3rd – 4th days after fertilization. Embryonic development of $H.~ savignyi$ is about 157 hours (7 days). Cleavage is unequal holoblastic. The embryonic developmental stages of $H.~ savignyi$ were compared with the result of a similar study of two other $Hyla$ species ($H.~orientalis$ and $H.~ annectans$) at various temperatures, and the possible temporal effect of the temperature and ovum size on the growth rate of these species was discussed.

Keywords

Fertilization, cleavage, gastrula, tadpole, growth rate, ovum size, gosner stages, hylidae

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