Effect of Various Extracts Obtained From Bee Pollen on L929 Fibroblast Cell Proliferation

Year 2024, , 1 - 7, 28.02.2024

İrfan Çınar , Çiğdem Sevim

https://doi.org/10.5281/zenodo.10779249

Abstract

AIM: Since ancient times bee pollen has been considered a good source of bioactive substances and energy, so its effects on tissue regeneration has been seen and evaluated. Considering the increasing demand for healthy and natural foods in recent years, it is unsurprising that bee pollen has been attracting commercial interest, making it one of the most widely consumed food supplements.
The substances contributing to tissue regeneration are essential for skin health and cosmetology. Cell culture studies have gained importance in tissue regeneration studies in recent years. Fibroblast cell line is frequently used in control studies, especially on the efficacy and toxicity of cosmetic products and chemical substances. In light of these data, we investigated the effects of bee pollen extracts on L929 fibroblast cells.
METHOD: Different concentrations of Turkey Bee pollen extract obtained with different solvents (hexane, dichloromethane, methanol, methanol + water, acetone, water) were applied to L929 fibroblast cells. The effects of pollen on cell proliferation were examined time-dependent by real-time cell counting system Xcelligence.
RESULT: In our studies, all bee pollen extracts in the fibroblast cell line increased cell proliferation. The methanol extract was especially observed to improve L929 cell proliferation by dose-dependent.
CONCLUSION: This study concluded that bee pollen extracts obtained at various concentrations and by different extractions do not cause any toxic effects on fibroblasts but significantly affect proliferation. From these results, bee pollen contributes to fibroblast proliferation and maybe a new target in cosmetics and developing drugs. However, more detailed work is needed to determine which pollen component is practical.

Keywords

L929, Fibroblast, Bee Pollen, Proliferation

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