INVESTIGATION OF THE EFFECTS OF PREGABALIN ON WOUND HEALING IN L929 FIBROBLAST CELLS

Abstract

Aim: Wound healing is a multifaceted, complex process consisting of sequential and interrelated phases including hemostasis/inflammation phase, proliferation phase and remodeling phase. Pregabalin (PGB), a gabapentin derivative, is an anticonvulsant agent with anti-inflammatory and antioxidant properties. Therefore, in this study, we aimed to show the effect of pregabalin on cell viability in L929 fibroblast cells and its effects on fibroblast migration and wound closure during the wound healing process. Materials And Methods: In this study, the effect of different concentrations of pregabalin on cell viability and proliferation in L929 skin fibroblast cells was investigated using MTT assay. In addition, a scratch wound healing model was established in L929 skin fibroblast cells and the effects of pregabalin concentrations that increase cell proliferation on wound healing in MTT assay were shown. At the end of the experiment, TGF-β1 levels of all groups were measured by ELISA method. Results: In our studies, it was observed that 100, 50, 25, 10 µM concentrations of pregabalin increased cell proliferation. In the scratch wound healing model, pregabalin at concentrations of 100 and 50 µM showed a significant closure compared to control and other groups. TGF-β1 levels were decreased in groups with good healing scores (50, 25 µM). Conclusion: Pregabalin has been shown to enhance wound healing in in vitro experiments. This effect needs to be evaluated holistically within the organ system in vivo. There is also a need for experimental and clinical studies to evaluate the wound healing effects and mechanism of pregabalin.

Keywords

• Wound Healing
• pregabalin
• scratch wound assay
• L929 cell line

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