Preparation and In Vitro Characterization of Alginate Beads Containing the Aqueous Extract of Artemisia dracunculus

Abstract

Objective: Artemisia dracunculus is a plant traditionally used in folk medicine, and its antioxidant, anti-inflammatory, and antihyperglycemic effects have been demonstrated in scientific studies. This study aims to prepare alginate beads containing the aqueous extract of Artemisia dracunculus and perform in vitro characterization studies. Methods: Four different extraction methods were tested to prepare the water extract, and the total phenolic content of the extracts was determined using the Folin–Ciocalteu method. The extract with the highest phenolic content was selected, and alginate beads were prepared by ionotropic gelation. The beads were dried using oven-drying or lyophilization methods. The beads were characterized in vitro (particle size, morphological analysis, encapsulation efficiency, swelling behavior, and FT-IR analyses). Results: The bead sizes were found to range from 0.73 ± 0.12 mm to 1.76 ± 0.13 mm, and it was determined that lyophilized beads were larger and more porous. The entrapment efficiency was found to range from 4.86% to 14.28%. Swelling studies have shown that lyophilized beads have a higher swelling capacity. FT-IR analysis revealed that the extracts were retained within the polymer matrix. Conclusion: Alginate beads containing Artemisia dracunculus aqueous extract have been successfully prepared and characterized in vitro.

Keywords

• Alginate
• Artemisia dracunculus
• beads
• in vitro characterization

Artemisia dracunculus Su Ekstresi İçeren Aljinat Boncukların Hazırlanması ve İn Vitro Karakterizasyonu

Abstract

Amaç: Artemisia dracunculus, geleneksel halk tıbbında kullanılan bir bitkidir ve antioksidan, antiinflamatuar ve antihiperglisemik etkileri bilimsel çalışmalarda gösterilmiştir. Bu çalışmanın amacı, Artemisia dracunculus'un su ekstresini içeren aljinat boncukları hazırlamak ve in vitro karakterizasyon çalışmaları yapmaktır. Yöntemler: Su ekstresinin hazırlanmasında dört farklı ekstraksiyon yöntemi denenmiş ve ekstrelerin toplam fenolik içerikleri Folin–Ciocalteu yöntemi ile belirlenmiştir. En yüksek fenolik içeriğe sahip olan ekstre seçilerek iyonotropik jelasyon ile aljinat boncukları hazırlanmıştır. Boncuklar etüvde kurutma veya liyofilizasyon yöntemleriyle kurutulmuştur. Boncukların in vitro karakterizasyonu (partikül boyutu, morfolojik analiz, enkapsülasyon etkinliği, şişme davranışı ve FT-IR analizleri) yapılmıştır. Şişme çalışmaları sonucunda, liyofilize boncukların şişme kapasitelerinin daha yüksek olduğu görülmüştür. Bulgular: Boncuk boyutları 0.73 ± 0.12 mm ile 1.76 ± 0.13 mm arasında bulunmuş ve liyofilize boncukların daha büyük ve daha gözenekli yapıda olduğu belirlenmiştir. Yükleme etkinliğinin %4.86 ile %14.28 arasında değiştiği bulunmuştur. FT-IR analizleri, ekstrelerin polimer matrisi içinde tutulduğunu ortaya koymuştur. Sonuç: Artemisia dracunculus su ekstresi içeren aljinat boncukları başarıyla hazırlanmış ve in vitro karakterize edilmiştir.

Keywords

• Aljinat
• Artemisia dracunculus
• boncuk
• in vitro karakterizasyon

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