Effect of Various Extracts Obtained From Bee Pollen on L929 Fibroblast Cell Proliferation

Year 2024, Volume: 1 Issue: 1, 1 - 7, 28.02.2024

İrfan Çınar , Çiğdem Sevim

https://doi.org/10.5281/zenodo.10779249

Abstract

AIM: Since ancient times bee pollen has been considered a good source of bioactive substances and energy, so its effects on tissue regeneration has been seen and evaluated. Considering the increasing demand for healthy and natural foods in recent years, it is unsurprising that bee pollen has been attracting commercial interest, making it one of the most widely consumed food supplements.
The substances contributing to tissue regeneration are essential for skin health and cosmetology. Cell culture studies have gained importance in tissue regeneration studies in recent years. Fibroblast cell line is frequently used in control studies, especially on the efficacy and toxicity of cosmetic products and chemical substances. In light of these data, we investigated the effects of bee pollen extracts on L929 fibroblast cells.
METHOD: Different concentrations of Turkey Bee pollen extract obtained with different solvents (hexane, dichloromethane, methanol, methanol + water, acetone, water) were applied to L929 fibroblast cells. The effects of pollen on cell proliferation were examined time-dependent by real-time cell counting system Xcelligence.
RESULT: In our studies, all bee pollen extracts in the fibroblast cell line increased cell proliferation. The methanol extract was especially observed to improve L929 cell proliferation by dose-dependent.
CONCLUSION: This study concluded that bee pollen extracts obtained at various concentrations and by different extractions do not cause any toxic effects on fibroblasts but significantly affect proliferation. From these results, bee pollen contributes to fibroblast proliferation and maybe a new target in cosmetics and developing drugs. However, more detailed work is needed to determine which pollen component is practical.

Keywords

L929, Fibroblast, Bee Pollen, Proliferation

References

• Almaraz-Abarca, N., M. D. Campos, J. A. Avila-Reyes, N. Naranjo-Jimenez, J. Herrera-Corral and L. S. Gonzalez-Valdez (2004). "Variability of antioxidant activity among honeybee-collected pollen of different botanical origin." Interciencia 29(10): 574-578.
• Bayram, N. E., Y. C. Gercek, S. Çelik, N. Mayda, A. Ž. Kostić, A. M. Dramićanin and A. Özkök (2021). "Phenolic and free amino acid profiles of bee bread and bee pollen with the same botanical origin–similarities and differences." Arabian Journal of Chemistry 14(3): 103004.
• Campos, M. G., S. Bogdanov, L. B. de Almeida-Muradian, T. Szczesna, Y. Mancebo, C. Frigerio and F. Ferreira (2008). "Pollen composition and standardisation of analytical methods." Journal of Apicultural Research 47(2): 154-161.
• Ebadi, P. and M. Fazeli (2021). "Evaluation of the potential in vitro effects of propolis and honey on wound healing in human dermal fibroblast cells." South African Journal of Botany 137: 414-422.
• Fengyan, S. (2014). "Dose Analysis of Methanol, Ethanol, Acetone and Glycerol to PC-12 Tumor Cells' Morphology and Growth." Agricultural Science & Technology 15(8).
• Forman, S., J. Kás, F. Fini, M. Steinberg and T. Ruml (1999). "The effect of different solvents on the ATP/ADP content and growth properties of HeLa cells." J Biochem Mol Toxicol 13(1): 11-15.
• Gethin, G. (2012). "Understanding the inflammatory process in wound healing." Br J Community Nurs Suppl: S17-18, S20, S22.
• Komosinska-Vassev, K., P. Olczyk, J. Kaźmierczak, L. Mencner and K. Olczyk (2015). "Bee pollen: chemical composition and therapeutic application." Evidence-Based Complementary and Alternative Medicine 2015.
• Nguyen, S. T., H. T.-L. Nguyen and K. D. Truong (2020). "Comparative cytotoxic effects of methanol, ethanol and DMSO on human cancer cell lines." Biomedical Research and Therapy 7(7): 3855-3859.
• Pascoal, A., S. Rodrigues, A. Teixeira, X. Feás and L. M. Estevinho (2014). "Biological activities of commercial bee pollens: Antimicrobial, antimutagenic, antioxidant and anti-inflammatory." Food and Chemical Toxicology 63: 233-239.
• Pereira, D. D. T., M. H. M. Lima-Ribeiro, N. T. de Pontes, A. M. D. Carneiro-Leao and M. T. D. Correia (2012). "Development of Animal Model for Studying Deep Second-Degree Thermal Burns." Journal of Biomedicine and Biotechnology.
• Peršurić, Ž. and S. K. Pavelić (2021). "Bioactives from bee products and accompanying extracellular vesicles as novel bioactive components for wound healing." Molecules 26(12): 3770.
• Reinke, J. M. and H. Sorg (2012). "Wound repair and regeneration." Eur Surg Res 49(1): 35-43.
• Rzepecka-Stojko, A., B. Pilawa, P. Ramos and J. Stojko (2012). "Antioxidative properties of bee pollen extracts examined by EPR spectroscopy." Journal of Apicultural Science 56(1): 23.
• Sankar, S., N. Mahooti-Brooks, L. Bensen, T. L. McCarthy, M. Centrella and J. A. Madri (1996). "Modulation of transforming growth factor beta receptor levels on microvascular endothelial cells during in vitro angiogenesis." The Journal of clinical investigation 97(6): 1436-1446.
• Shier, W. T. (1988). "Studies on the mechanisms of mammalian cell killing by a freeze-thaw cycle: conditions that prevent cell killing using nucleated freezing." Cryobiology 25(2): 110-120.
• Sinno, H. and S. Prakash (2013). "Complements and the wound healing cascade: an updated review." Plast Surg Int 2013: 146764.
• Stammati, A., F. Zampaglioni and F. Zucco (1997). "Furaltadone cytotoxicity on three cell lines in the presence or absence of DMSO: comparison with furazolidone." Cell Biol Toxicol 13(2): 125-130.
• Velnar, T., T. Bailey and V. Smrkoli (2009). "The Wound Healing Process: an Overview of the Cellular and Molecular Mechanisms." Journal of International Medical Research 37(5): 1528-1542.
• Zizic, J. B., N. L. Vukovic, M. B. Jadranin, B. D. Andelkovic, V. V. Tesevic, M. M. Kacaniova, S. B. Sukdolak and S. D. Markovic (2013). "Chemical composition, cytotoxic and antioxidative activities of ethanolic extracts of propolis on HCT-116 cell line." J Sci Food Agric 93(12): 3001-3009