Evaluation of Wound Healing Potential of Galangin on L929 Mouse Fibroblast Cell Lines Using In Vitro Scratch Assay

Year 2024, Volume: 1 Issue: 3, 99 - 104, 28.10.2024

Pelin Aydın , Zeynep Karaköy , Hamza Halıcı

https://doi.org/10.5281/zenodo.13976728

Abstract

AIM: The aim of this study was to investigate the effect of galangin, which is known to have anti-inflammatory and antioxidant properties, on cell proliferation and migration in a scratch wound healing model in L929 cell line.
MATERIALS AND METHODS: In this study, we investigated the effects of different concentrations of galangin on cell proliferation and viability in L929 cell line using MTT method. We then examined the effects of different concentrations of galangin on wound closure in the wound line created by scratch wound healing test. At 0, 12, 24 and 36 hours, microscopic images of the wound line were taken. Wound closure rates were calculated and percent wound closure graph was created. At the end of our study, TGF-β levels of all groups were measured using ELISA kits.
RESULTS: According to the viability percentages determined by MTT method in L929 cells, 10, 25 and 50 µM concentrations of galangin significantly increased cell viability at 24, 48 and 72 hours. In the scratch wound healing model in which these 3 concentrations of galangin were applied as treatment, it was observed that 25 and 50 µM concentrations of galangin showed a near complete closure at the end of the experiment. When the measured TGF-β levels were analyzed, a significant decrease was observed in the galangin-treated groups compared to the control group.
CONCLUSION: In the in vitro scratch wound healing model, the observed reduction in TGF-β levels at the conclusion of the experiment in the galangin-treated groups, compared to the control group, suggests that the healing process is nearing completion, resulting in a concomitant decrease in cytokine release. These findings are consistent with the percentage closure rates assessed microscopically across the different treatment groups.

Keywords

Galangin, migration, mouse skin fibroblast, scratch wound assay

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