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Effect of Experimental Morphine Dependence and Morphine Withdrawal on Bladder Smooth Muscle Contraction

Year 2022, Volume: 2 Issue: 1, 1 - 10, 16.06.2022

Abstract

Although the effects of morphine on renal functions and bladder have been investigated, it has not been studied yet whether the long-term morphine dependence and related withdrawal syndrome have a different effect on the bladder. Aim of the study is to investigate the effects of morphine addiction and morphine withdrawal on bladder smooth muscle. Adult male Wistar-Albino rats (n:30) included in the study were randomly divided into 3 groups. 10 mg/kg 0.9% NaCl solution was injected subcutaneously once a day for 7 days. 10 mg/kg morphine was injected subcutaneously once a day for 7 days in morphine and morphine-withdrawal group. After two hours of the last injections, 3 mg/kg 0.9% NaCl solution was injected intraperitoneally in both control and morphine groups, while 3 mg/kg naloxone for morphine-withdrawal group. Then the behavior of the animals was observed for thirty hours. Afterwards, the bladders were rapidly extracted and their tension was recorded in the isolated organ bath. Morphine withdrawal behaviors were observed in rats in the morphine withdrawal group. When the bladder contractions of the groups were examined, no statistically significant difference was found between the tension values (p>0.05). In summary the study carried out in rats with morphine addiction, it was observed that being addicted to morphine or being on morphine withdrawal did not affect bladder contractions.

References

  • Acevedo, C. G., Tamayo, L. ve Contreras, E. (1986). Effects of morphine in the isolated mouse urinary bladder. Gen Pharmacol, 17(4), 449-452.
  • Akan, M., Toplu, S. A. ve Cumurcu, H. B. (2020). Naltrekson implanta bağlı gelişmiş lokal komplikasyonlara multidisipliner yaklaşım: Olgu sunumu. Current Addiction Research, 4(1), 28-34.
  • Bender, A. M., Clark, M. J., Agius, M. P., Traynor, J. R. ve Mosberg, H. I. (2014) Synthesis and evaluation of 4-substituted piperidines and piperazines as balanced affinity μ opioid receptor (MOR) agonist/δ opioid receptor (DOR) antagonist ligands. Bioorg Med Chem Lett, 24(2), 548-551.
  • Broseta I., Rodríguez-Arias. M., Stinus, L., ve Miñarro, J. (2002) Ethological analysis of morphine withdrawal with different dependence programs in male mice. Prog Neuropsychopharmacol Biol Psychiatry, 26(2), 335-347.
  • Carpenter, F. G. (1986). Micturition in naive and morphine-dependent rats. Br J Pharmacol, 87(4), 725-731.
  • Dray, A. ve Metsch R. (1984). Inhibition of urinary bladder contractions by a spinal action of morphine and other opioids. J Pharmacol Exp Ther, 231(2), 254-260.
  • Fennessy, M. R., Heimans, R. L. ve Rand, M. J. (1969). Comparison of effect of morphine-like analgesics on transmurally stimulated guinea-pig ileum. Br J Pharmacol, 37(2), 436-449.
  • Gellert, V. F. ve Holtzman, S. G. (1978). Development and maintenance of morphine tolerance and dependence in the rat by scheduled access to morphine drinking solutions. J. Pharmacol. Exp. Ther. 205, 536-546.
  • Kaya, E., Akpınar, D. ve Akpınar, H. (2019). Bağımlılığın patofizyolojisi. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi, 6(3), 166-170.
  • Kiefer, F. ve Mann, K. (2005). New achievements and pharmacotherapeutic approaches in the treatment of alcohol dependence. Eur J Pharmacol, 526(1-3), 163-171.
  • Kontani, H., Nakagawa, M. ve Sakai, T. (1989). Effects of central nervous system-acting drugs on urinary bladder contraction in unanesthetized rats. Jpn J Pharmacol, 50(3), 327-332.
  • Kranzler, H. R. ve Ciraulo, D. A. (2013). Clinical manual of addiction psychopharmacology. American Psychiatric Press.
  • Listos, J., Łupina, M., Talarek, S., Mazur, A., Orzelska-Górka, J. ve Kotlińska, J. (2019). The mechanisms involved in morphine addiction: an overview. Int J Mol Sci, 20(17), 4302.
  • Shimizu, I., Kawashima, K., Ishii, D. ve Oka, M. (2000). Effects of (+)-pentazocine and 1,3-di-o-tolylguanidine (DTG), sigma (sigma) ligands, on micturition in anaesthetized rats. Br J Pharmacol, 131(3), 610-616.
  • World Health Organization. (2009) Department of Mental Health, Substance abuse. Guidelines for the psychosocially assisted pharmacological treatment of opioid dependence. Available from https://www.who.int/substance_abuse/publications/opioid_dependence_guidelines.pdf
  • Zhou, P., Li, Y., Yong, Z., Yan, H., Su, R. ve Gong, Z. (2016). Effects of thienorphine on the contraction of isolated ureter and bladder of guinea pigs. Eur J Pharmacol, 789, 144-151.

Deneysel Morfin Bağımlılığının ve Morfin Çekilmesinin Mesane Düz Kas Kontraksiyonuna Etkisi

Year 2022, Volume: 2 Issue: 1, 1 - 10, 16.06.2022

Abstract

Morfinin renal fonksiyonlar ve mesane üzerine etkileri araştırılmış olmakla birlikte morfinin uzun süreli kullanımı sonucu oluşan bağımlılık ve buna bağlı yoksunluk sendromunun mesane üzerinde farklı etki oluşturup oluşturmadığı henüz çalışılmamıştır. Bu çalışmanın amacı, morfin bağımlılığının ve morfin yoksunluğunun mesane düz kası üzerine olan etkisini araştırmaktır.Çalışmaya alınan yetişkin erkek Wistar-Albino ratlar (n:30) rastgele 3 gruba ayrıldı. Kontrol grubuna 7 gün boyunca 10 mg/kg %0.9 NaCl çözeltisi cilt altı yoldan enjekte edildi. Benzer şekilde, morfin ve morfin yoksunluğu grubuna ise 10 mg/kg morfin verildi. Son enjeksiyondan 2 saat sonra morfin yoksunluğu grubuna intraperitoneal yoldan 3 mg/kg nalokson verilirken, kontrol ve morfin gruplarına %0.9 NaCl verildi ve yarım saat boyunca hayvanların davranışları gözlendi. Sonrasında ise izole organ banyosunda gerimlerini kaydetmek amacıyla hayvanların mesaneleri ayrıldı. Morfin çekilmesi grubundaki sıçanlarda morfin yoksunluk davranışları gözlemlenmiştir. Grupların mesane kontraksiyonları incelendiğinde ise gerim değerleri arasında istatistiksel olarak anlamlı bir farklılık bulunmamıştır (p>0.05). Sonuç olarak, morfin bağımlılığı oluşturulmuş sıçanlarda yapılan çalışma sonucunda morfin bağımlılığı ya da morfin yoksunluğunun mesane kontraksiyonlarını etkilemediği görülmüştür.

References

  • Acevedo, C. G., Tamayo, L. ve Contreras, E. (1986). Effects of morphine in the isolated mouse urinary bladder. Gen Pharmacol, 17(4), 449-452.
  • Akan, M., Toplu, S. A. ve Cumurcu, H. B. (2020). Naltrekson implanta bağlı gelişmiş lokal komplikasyonlara multidisipliner yaklaşım: Olgu sunumu. Current Addiction Research, 4(1), 28-34.
  • Bender, A. M., Clark, M. J., Agius, M. P., Traynor, J. R. ve Mosberg, H. I. (2014) Synthesis and evaluation of 4-substituted piperidines and piperazines as balanced affinity μ opioid receptor (MOR) agonist/δ opioid receptor (DOR) antagonist ligands. Bioorg Med Chem Lett, 24(2), 548-551.
  • Broseta I., Rodríguez-Arias. M., Stinus, L., ve Miñarro, J. (2002) Ethological analysis of morphine withdrawal with different dependence programs in male mice. Prog Neuropsychopharmacol Biol Psychiatry, 26(2), 335-347.
  • Carpenter, F. G. (1986). Micturition in naive and morphine-dependent rats. Br J Pharmacol, 87(4), 725-731.
  • Dray, A. ve Metsch R. (1984). Inhibition of urinary bladder contractions by a spinal action of morphine and other opioids. J Pharmacol Exp Ther, 231(2), 254-260.
  • Fennessy, M. R., Heimans, R. L. ve Rand, M. J. (1969). Comparison of effect of morphine-like analgesics on transmurally stimulated guinea-pig ileum. Br J Pharmacol, 37(2), 436-449.
  • Gellert, V. F. ve Holtzman, S. G. (1978). Development and maintenance of morphine tolerance and dependence in the rat by scheduled access to morphine drinking solutions. J. Pharmacol. Exp. Ther. 205, 536-546.
  • Kaya, E., Akpınar, D. ve Akpınar, H. (2019). Bağımlılığın patofizyolojisi. Muğla Sıtkı Koçman Üniversitesi Tıp Dergisi, 6(3), 166-170.
  • Kiefer, F. ve Mann, K. (2005). New achievements and pharmacotherapeutic approaches in the treatment of alcohol dependence. Eur J Pharmacol, 526(1-3), 163-171.
  • Kontani, H., Nakagawa, M. ve Sakai, T. (1989). Effects of central nervous system-acting drugs on urinary bladder contraction in unanesthetized rats. Jpn J Pharmacol, 50(3), 327-332.
  • Kranzler, H. R. ve Ciraulo, D. A. (2013). Clinical manual of addiction psychopharmacology. American Psychiatric Press.
  • Listos, J., Łupina, M., Talarek, S., Mazur, A., Orzelska-Górka, J. ve Kotlińska, J. (2019). The mechanisms involved in morphine addiction: an overview. Int J Mol Sci, 20(17), 4302.
  • Shimizu, I., Kawashima, K., Ishii, D. ve Oka, M. (2000). Effects of (+)-pentazocine and 1,3-di-o-tolylguanidine (DTG), sigma (sigma) ligands, on micturition in anaesthetized rats. Br J Pharmacol, 131(3), 610-616.
  • World Health Organization. (2009) Department of Mental Health, Substance abuse. Guidelines for the psychosocially assisted pharmacological treatment of opioid dependence. Available from https://www.who.int/substance_abuse/publications/opioid_dependence_guidelines.pdf
  • Zhou, P., Li, Y., Yong, Z., Yan, H., Su, R. ve Gong, Z. (2016). Effects of thienorphine on the contraction of isolated ureter and bladder of guinea pigs. Eur J Pharmacol, 789, 144-151.
There are 16 citations in total.

Details

Primary Language Turkish
Subjects Psychiatry
Journal Section Research Articles
Authors

Rabia Solak This is me 0000-0003-1547-5351

Faik Özdengül This is me 0000-0001-9670-0789

Zülfikare Işık Solak Görmüş This is me 0000-0001-6762-6225

Oğuzhan Yaylalı This is me 0000-0003-2064-5773

Hasan Bakay This is me 0000-0002-8864-2942

Publication Date June 16, 2022
Submission Date February 14, 2022
Published in Issue Year 2022 Volume: 2 Issue: 1

Cite

APA Solak, R., Özdengül, F., Solak Görmüş, Z. I., Yaylalı, O., et al. (2022). Deneysel Morfin Bağımlılığının ve Morfin Çekilmesinin Mesane Düz Kas Kontraksiyonuna Etkisi. Current Research and Reviews in Psychology and Psychiatry, 2(1), 1-10.