T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Mustafa Ergül

doi:10.17776/csj.1238418

EN

T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells

Abstract

Accumulating evidence reports that T-type calcium channels play crucial roles in tumor formation and development. However, the roles of inhibiting calcium channels in tumor cells with various inhibitors in tumor progression remain unclear. This study aimed to investigate the cytotoxic and apoptotic effects of NNC 55-0396, a T-type calcium channel inhibitor, against SNU-1 gastric cancer cells. Cytotoxic and apoptotic effects of NNC 55-0396 were evaluated by the XTT assay and flow cytometry. The results showed that NNC 55-0396 had concentration-dependent cytotoxicity in SNU-1 cells and its the half-maximal inhibitory concentration (IC50) value was calculated as 4.17 µM. The results of the Annexin V experiments also showed that this inhibitor significantly increased apoptosis in SNU-1 cells. In conclusion, these results demonstrated that NNC 55-0396 induces cytotoxic effects by increasing apoptosis in gastric cancer cells. However, further research is required for its use as a possible therapeutic agent in the treatment of gastric cancer.

Keywords

Gastric cancer, NNC 55-0396, Cytotoxicity, Apoptosis.

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Details

Primary Language

English

Subjects

Pharmacology and Pharmaceutical Sciences

Journal Section

Research Article

Authors

Mustafa Ergül ^*
0000-0003-4303-2996
Türkiye

Publication Date

June 30, 2023

Submission Date

January 18, 2023

Acceptance Date

June 7, 2023

Published in Issue

Year 2023 Volume: 44 Number: 2

DOI

https://doi.org/10.17776/csj.1238418

IZ

https://izlik.org/JA37TK34AX

APA

Ergül, M. (2023). T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal, 44(2), 254-257. https://doi.org/10.17776/csj.1238418

AMA

1.Ergül M. T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. CSJ. 2023;44(2):254-257. doi:10.17776/csj.1238418

Chicago

Ergül, Mustafa. 2023. “T-Type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells”. Cumhuriyet Science Journal 44 (2): 254-57. https://doi.org/10.17776/csj.1238418.

EndNote

Ergül M (June 1, 2023) T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. Cumhuriyet Science Journal 44 2 254–257.

IEEE

[1]M. Ergül, “T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells”, CSJ, vol. 44, no. 2, pp. 254–257, June 2023, doi: 10.17776/csj.1238418.

ISNAD

Ergül, Mustafa. “T-Type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells”. Cumhuriyet Science Journal 44/2 (June 1, 2023): 254-257. https://doi.org/10.17776/csj.1238418.

JAMA

1.Ergül M. T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. CSJ. 2023;44:254–257.

MLA

Ergül, Mustafa. “T-Type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells”. Cumhuriyet Science Journal, vol. 44, no. 2, June 2023, pp. 254-7, doi:10.17776/csj.1238418.

Vancouver

1.Mustafa Ergül. T-type Calcium Channel Blocker, NNC 55-0396, Suppress Cell Proliferation and Promote Apoptosis in SNU-1 Gastric Cancer Cells. CSJ. 2023 Jun. 1;44(2):254-7. doi:10.17776/csj.1238418

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