Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties

Derya Kılıçaslan

doi:10.17776/csj.1414168

EN

Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties

Abstract

A novel donepezil-squaric acid (DS) hybrid compound was designed, synthesized and biologically evaluated as multi-target-directed ligands against neurodegenerative disease by fusing a fragment of donepezil (D) and squaric acid (S). This study focuses on investigating the binding mechanism of double-stranded fish sperm DNA (Fsds-DNA) with DS and S. The interaction between DS and S with Fsds-DNA was explored using spectrophotometric and viscometric methods. Besides, DNA binding constants (Kb) were determined. The results reveal that DS binds to Fsds-DNA via the minor groove binding mode. The hybrid molecule demonstrates potent inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Furthermore, it exhibits significant antioxidant activity, surpassing the scavenging ability of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals compared to squaric acid alone. In conclusion, these results suggest that the hybrid molecule may serve as a potential multifunctional agent for the treatment of Alzheimer’s disease.

Keywords

Donepezil, Squaric acid, DNA binding, Acetylcholinesterase, Butyrylcholinesterase

Supporting Institution

Kahramanmaras Sütçü İmam University Research Unit

Project Number

2019/3-21 M

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Details

Primary Language

English

Subjects

Analytical Spectrometry

Journal Section

Research Article

Authors

Derya Kılıçaslan ^*
0000-0001-7830-8214
Türkiye

Publication Date

June 30, 2024

Submission Date

January 3, 2024

Acceptance Date

June 14, 2024

Published in Issue

Year 2024 Volume: 45 Number: 2

DOI

https://doi.org/10.17776/csj.1414168

IZ

https://izlik.org/JA74MH49TC

APA

Kılıçaslan, D. (2024). Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties. Cumhuriyet Science Journal, 45(2), 216-226. https://doi.org/10.17776/csj.1414168

AMA

1.Kılıçaslan D. Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties. CSJ. 2024;45(2):216-226. doi:10.17776/csj.1414168

Chicago

Kılıçaslan, Derya. 2024. “Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties”. Cumhuriyet Science Journal 45 (2): 216-26. https://doi.org/10.17776/csj.1414168.

EndNote

Kılıçaslan D (June 1, 2024) Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties. Cumhuriyet Science Journal 45 2 216–226.

IEEE

[1]D. Kılıçaslan, “Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties”, CSJ, vol. 45, no. 2, pp. 216–226, June 2024, doi: 10.17776/csj.1414168.

ISNAD

Kılıçaslan, Derya. “Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties”. Cumhuriyet Science Journal 45/2 (June 1, 2024): 216-226. https://doi.org/10.17776/csj.1414168.

JAMA

1.Kılıçaslan D. Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties. CSJ. 2024;45:216–226.

MLA

Kılıçaslan, Derya. “Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties”. Cumhuriyet Science Journal, vol. 45, no. 2, June 2024, pp. 216-2, doi:10.17776/csj.1414168.

Vancouver

1.Derya Kılıçaslan. Donepezil-Squaric Acid Hybrid: Synthesis, Characterization and Investigation of Anticholinesterase Inhibitory, DNA Binding and Antioxidant Properties. CSJ. 2024 Jun. 1;45(2):216-2. doi:10.17776/csj.1414168

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