Evaluation of Bioactivity of Ethanol and Water Extracts and Determination of Nutrient Concentrations of Laurel (Laurus nobilis) Plants Grown in İzmir Province
Abstract
This study was conducted to determination of ethanol and water extracts obtained from the leaves of Laurus nobilis (Laurel) plant of the general content by GC-MS analysis, antimicrobial activities of these extracts on some microorganisms, total antioxidant levels (TAS), total oxidant levels (TOS), oxidative stress index (OSI) values, anti-carcinogenic effects on various cell lines and macro and micronutrient concentrations of Laurel (Laurus nobilis) plants cultivated for decorative purposes in parks and gardens of Izmir province of Turkey. TAS, TOS, and OSI values were determined with the aid of Rel Assay Diagnostics kits. In antimicrobial activity analysis, the minimum inhibition concentration (MIC) of the plant extracts against microorganisms was determined by microdilution broth method. Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Escherichia coli (ATCC 25922), Bacillus cereus (ATCC11778), Candida albicans (ATCC 10231) and Candida tropicalis (DSM11953) microorganisms were used in this analysis. Furthermore, cytotoxic activities of plant extracts were determined by XTT method in normal rat fibroblast cell line, HUVEC, and 2 different human cancer cell lines. At the end of the study, it was found that ethanol and water extracts of the Laurel plants had no antimicrobial activity, low oxidative stress index and good antioxidant activity, and moderate cytotoxic activity on experimented cell lines. Nitrogen (N), Phosphorus (P), Potassium (P), Calcium (Ca), Magnesium (Mg), Iron (Fe), Zinc (Zn), Manganese (Mn) and Copper (Cu) concentrations of plant extracts were respectively identified as 1.68% N, 0.72% P, 1.12% K, 2.40% Ca, 0.45% Mg, 147.4 mg/kg Fe, 13.6 mg/kg Zn, 326.3 mg/kg Mn and 27.8 mg/kg Cu.
Keywords
Antimicrobial activity, Antioxidant activity, Nutrient, Laurus nobilis (Laurel).
Supporting Institution
Scientific Research Projects Department of Sivas Cumhuriyet University
Project Number
YMYO-007 and V-066
Thanks
The authors would like to thank Dr. Mehmet Atas for his aid and support, and Dr. Mustafa Sevindik for his aid in plant species identification.
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