In Vitro Antioxidant Assessment of Methanol and Water Extracts of Anthemis kotschyana Boiss. var. kotschyana
Abstract
The genus Anthemis, with approximately 130 species globally, is significant within the Asteraceae family and is widely used in traditional medicine. Studies on the biological activity of Anthemis kotschyana, an endemic species found in Turkey, are limited. This study investigated the chemical constituents and antioxidant activity of Anthemis kotschyana using two different extracts, methanol and water. The major components of the plant were determined by GC‒MS. According to the data obtained, the major component was found to be "2-propenoic acid, a tridecyl ester" in the water extract and "cyclododecane" in the methanol extract. When the DPPH and ABTS radical scavenging activities were analysed, the methanol extract was found to be more active than the water extract in the DPPH experiment, while the opposite was observed in the ABTS experiment. The TPC was greater than the TFC based on the acquired results. The TPC of the water extract was 83.7 ± 15.6 mg GAE/g, the TPC of the MeOH extract was 170.7 ± 17.4 mg GAE/g, and the TFC of the water extract was 24.3 ± 7.2 QE/g, while the TFC of the MeOH extract was 65.6 ± 5.3 QE/g. For both substances, the amount of methanol extract was greater than that of the water extract. In conclusion, further studies on this species, known for its antioxidant activity and potential as a source of phenolic compounds, could support its use as a therapeutic agent in the future.
Keywords
Anthemis kotschyana, Antioxidant, DPPH, ABTS, Medicinal plant.
Thanks
I would like to thank Prof. Dr. Hüseyin Aşkın Akpulat for identifying of the plant.
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