Determination of Total Phenolic Amounts of Chloroform, Acetone and Methanol Extracts of Sargassum sp., Ulva sp. and Schizochytrium sp.
Abstract
The present study was aimed to determine the total phenolic amounts of the bioactive contents resulting from the extraction of Sargassum sp., Ulva sp. and Schizochytrium sp. with different solvents such as chloroform, acetone and methanol. Total phenolic content was determined using the Folin-Ciocalteu method. Samples (12.5%) were extracted with chloroform, acetone, and methanol, then filtered and the solvents were evaporated. Absorbance was measured at 760 nm. Total phenolic content was expressed as gallic acid equivalents (GAE). The total phenolic contents obtained from chloroform, acetone and methanol extracts of Sargassum sp., Ulva sp. and Schizochytrium sp. were determined as 136.40±1.93 mg GAE/g extract -3.61±0.18 mg GAE/g extract -7.83±0.03 mg GAE/g extract, 5.25±0.07 mg GAE/g extract -4.27±0.1 mg GAE/g extract -14.5±0.06 mg GAE/g extract and 15.7±0.27 mg GAE/g extract -2.6±0.04 mg GAE/g extract -14.84±0.11 mg GAE/g extract, respectively. Differences between total phenolic values were found to be statistically significant (p<0.05). The extraction yields obtained from chloroform, acetone and methanol extracts of Sargassum sp., Ulva sp. and Schizochytrium sp. were determined as 2.2±0.06%-4.35±0.07%-6.68±0.14%, 2.96±0.02%-4.33±0.09%-9.74±0.16% and 14.96±0.24%-10.03±0.004%-19.03±0.3%, respectively (p<0.05). Biochemical parameters such as dry matter, protein, ash and lipid of Sargassum sp., Ulva sp. and Schizochytrium sp. were found to be 94.05±0.01% -20.92±0.35% -26.55±1.77% -1.36±0.01%, 94.1±0.005%-17.04±0.04%-28.11±0.06% -2.66±0.05% and 95.1±0.05%- 23.01±0.07%- 9.59±0.787%- 25.5±0.21%. The soluble protein concentrations of Sargassum sp., Ulva sp. and Schizochytrium sp. in distilled water were determined as 66.45±0.5mg/g, 83.38±0.88 mg/g and 115.95±0.89 mg/g, respectively. In conclusion, chloroform and methanol extracts of Schizochytrium sp. had good extraction yield and phenolic content. On the other hand, the chloroform extract of Sargassum sp. had the highest phenolic content, while the extraction yield was at the lowest level. Acetone extracts of the 3 species tested exhibited low phenolic activity.
Keywords
Sargassum sp., Ulva sp., Schizochytrium sp., Different solvents, Total phenolics
Ethical Statement
Since no human or animal subjects were used in this study, ethical approval was not required.
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