Glutathione Reductase: Purification and Characterization from Quail Liver

Sarkat Salim Mohammed Taher; Yusuf Temel; Mehmet Çiftci

doi:10.17776/csj.1758754

Glutathione Reductase: Purification and Characterization from Quail Liver

Abstract

Glutathione reductase (glutathione: NADP⁺ oxidoreductase, EC 1.8.1.7; GR) maintains the cellular level of reduced glutathione (GSH) by catalyzing the reduction of oxidized glutathione (GSSG) through a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reaction. Therefore, GR plays a crucial role in the cellular antioxidant defense system. In the present study, GR enzyme was purified from quail liver tissue in two steps using salting-out (ammonium sulfate precipitation) followed by affinity chromatography on 2',5'-ADP Sepharose-4B. The results indicated that the enzyme was purified 142.18-fold with a yield of 13.6% and a specific activity of 22.75 EU/mg protein. Enzyme activity was determined spectrophotometrically. The molecular mass of the GR enzyme subunits in its native state was estimated to be approximately 59 kDa by SDS–PAGE analysis. Characterization studies revealed that the optimum pH of the enzyme was 8.0 in K-phosphate buffer, while the optimum ionic strength was determined as 600 mM in the same buffer system. The optimum temperature for enzyme activity was found to be 85°C, and the enzyme exhibited maximum stability at pH 8.0 in Tris–HCl buffer. Kinetic parameters of the enzyme were also determined. The K_m and V_max values for NADPH, one of the physiological substrates of GR, were calculated as 0.109 mM and 0.603 EU/mL, respectively. For GSSG, the K_M and Vmax values were determined to be 0.044 mM and 0.272 EU/mL, respectively.

Keywords

Characterization, Glutathione reductase, Purification, Quail liver

Project Number

FEF.3.16.002

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Details

Primary Language

English

Subjects

Enzymes

Journal Section

Research Article

Authors

Sarkat Salim Mohammed Taher
0009-0007-7644-554X
Türkiye

Yusuf Temel ^*
0000-0001-8148-3718
Türkiye

Mehmet Çiftci
0000-0002-1748-3729
Türkiye

Publication Date

June 29, 2026

Submission Date

August 5, 2025

Acceptance Date

May 11, 2026

Published in Issue

Year 2026 Volume: 47 Number: 3

DOI

https://doi.org/10.17776/csj.1758754

IZ

https://izlik.org/JA88NC79MJ

APA

Taher, S. S. M., Temel, Y., & Çiftci, M. (2026). Glutathione Reductase: Purification and Characterization from Quail Liver. Cumhuriyet Science Journal, 47(3), 496-503. https://doi.org/10.17776/csj.1758754

AMA

1.Taher SSM, Temel Y, Çiftci M. Glutathione Reductase: Purification and Characterization from Quail Liver. CSJ. 2026;47(3):496-503. doi:10.17776/csj.1758754

Chicago

Taher, Sarkat Salim Mohammed, Yusuf Temel, and Mehmet Çiftci. 2026. “Glutathione Reductase: Purification and Characterization from Quail Liver”. Cumhuriyet Science Journal 47 (3): 496-503. https://doi.org/10.17776/csj.1758754.

EndNote

Taher SSM, Temel Y, Çiftci M (June 1, 2026) Glutathione Reductase: Purification and Characterization from Quail Liver. Cumhuriyet Science Journal 47 3 496–503.

IEEE

[1]S. S. M. Taher, Y. Temel, and M. Çiftci, “Glutathione Reductase: Purification and Characterization from Quail Liver”, CSJ, vol. 47, no. 3, pp. 496–503, June 2026, doi: 10.17776/csj.1758754.

ISNAD

Taher, Sarkat Salim Mohammed - Temel, Yusuf - Çiftci, Mehmet. “Glutathione Reductase: Purification and Characterization from Quail Liver”. Cumhuriyet Science Journal 47/3 (June 1, 2026): 496-503. https://doi.org/10.17776/csj.1758754.

JAMA

1.Taher SSM, Temel Y, Çiftci M. Glutathione Reductase: Purification and Characterization from Quail Liver. CSJ. 2026;47:496–503.

MLA

Taher, Sarkat Salim Mohammed, et al. “Glutathione Reductase: Purification and Characterization from Quail Liver”. Cumhuriyet Science Journal, vol. 47, no. 3, June 2026, pp. 496-03, doi:10.17776/csj.1758754.

Vancouver

1.Sarkat Salim Mohammed Taher, Yusuf Temel, Mehmet Çiftci. Glutathione Reductase: Purification and Characterization from Quail Liver. CSJ. 2026 Jun. 1;47(3):496-503. doi:10.17776/csj.1758754

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

Glutathione Reductase: Purification and Characterization from Quail Liver

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References

Details

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Published in Issue

DOI

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