Investigation of the Effect of the Tri-Set Training Method on Plasma mir-1, mir-128a, mir-133, and mir-206 Levels
Abstract
The miRNAs (Micro Ribonucleic Acid) mir-1, mir-128a, mir-133, and mir-206 are specifically associated with muscle hypertrophy and play key roles in its development. The aim of this study is to investigate the effects of the tri-set training method on the expression levels of mir-1, mir-128a, mir-133, and mir-206. Twenty-six volunteer athletes participated in the study. Anthropometric measurements were performed on the first day. Blood samples were collected before training and a 12-repetition maximum test (12RM) protocol was applied. On the second day, a maximum of 12 repetitions of Full Squat (SQ), Deadlift (DL), and 45° Leg Press (LP) movements were tested. On the third day, the training protocol was applied. On the fourth day, post-training blood samples were obtained. Expression levels of mir-1, mir-128a, and mir-206 increased after training, but these changes were not statistically significant (P<0.05). Correlation analysis of pre- and post-training expression levels shown significant positive correlations between mir-1 (r= 0.760) and mir-128a (r= 0.737). Tri-set training elevated the levels of mir-1, mir-128a and mir-206. These results suggest a potential relationship between muscle hypertrophy and these miRNAs. Further investigations with larger sampling sizes are required to confirm these findings.
Keywords
miRNA, Muscle hypertrophy, Resistance training, Tri set training.
Supporting Institution
Project Number
Ethical Statement
Thanks
References
- Schoenfeld, B. (2021). Science and development of muscle hypertrophy. Human Kinetics.
- Motohashi, N., Alexander, M. S., Shimizu-Motohashi, Y., Myers, J. A., Kawahara, G., & Kunkel, L. M. (2013). Regulation of IRS1/Akt insulin signaling by microRNA-128a during myogenesis. Journal of Cell Science, 126(12), 2678–2691. https://doi.org/10.1242/jcs.119966
- Silva, G. J. J., Bye, A., El Azzouzi, H., & Wisløff, U. (2017). MicroRNAs as important regulators of exercise adaptation. Progress in Cardiovascular Diseases, 60(1), 130–151. https://doi.org/10.1016/j.pcad.2017.06.003
- Townley-Tilson, W. H. D., Callis, T. E., & Wang, D. (2010). MicroRNAs 1, 133, and 206: Critical factors of skeletal and cardiac muscle development, function, and disease. International Journal of Biochemistry and Cell Biology, 42(8), 1252–1255. https://doi.org/10.1016/j.biocel.2009.03.002
- Zhang, T., Birbrair, A., Wang, Z. M., Messi, M. L., Marsh, A. P., Leng, I., Nicklas, B. J., & Delbono, O. (2015). Improved knee extensor strength with resistance training associates with muscle specific miRNAs in older adults. Experimental Gerontology, 62, 7–13. https://doi.org/10.1016/j.exger.2014.12.014
- Ge, Y., & Chen, J. (2011). MicroRNAs in skeletal myogenesis. Cell Cycle, 10(3), 441–448. https://doi.org/10.4161/cc.10.3.14710
- Moresi, V., Marroncelli, N., Coletti, D., & Adamo, S. (2015). Regulation of skeletal muscle development and homeostasis by gene imprinting, histone acetylation and microRNA. Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1849(3), 309–316. https://doi.org/10.1016/j.bbagrm.2015.01.002
- Barbiera, A., Pelosi, L., Sica, G., & Scicchitano, B. M. (2020). Nutrition and microRNAs: Novel insights to fight sarcopenia. Antioxidants, 9(10), 1–23. https://doi.org/10.3390/antiox9100951
- Drummond, M. J., Mccarthy, J. J., Fry, C. S., Esser, K. A., & Rasmussen, B. B. (2008). Aging differentially affects human skeletal muscle microRNA expression at rest and after an anabolic stimulus of resistance exercise and essential amino acids. American Journal of Physiology-Endocrinology and Metabolism, 295(6), 1333–1340. https://doi.org/10.1152/ajpendo.90562.2008
- Rao, P. K., Kumar, R. M., Farkhondeh, M., Baskerville, S., & Lodish, H. F. (2006). Myogenic factors that regulate expression of muscle-specific microRNAs. Proceedings of the National Academy of Sciences, 103(23), 8721–8726. https://doi.org/10.1073/pnas.0602831103