Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy

Muhammet Kemal Deveçeker; Süleyman Adıyaman; Sevda Akay Sazaklioglu

doi:10.17776/csj.1904980

Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy

Abstract

Folic acid (FA) is a vitamin that is critical for DNA synthesis, cell division, and red blood cell production. A UV–Vis spectrophotometric method was developed for the quantitative determination of FA, and its performance was evaluated in detail. The method exhibited a clear linear response within the concentration range of 9 µM to 0.1 mM, with a coefficient of determination exceeding 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were calculated as 3 µM and 9 µM, respectively, indicating that the sensitivity of the method is adequate for routine laboratory analysis. In addition to method validation, the influence of Vitamin B₁₂ (Vit B₁₂), lactose monohydrate (LM), leucine (Leu), and proline (Pro) was investigated to evaluate their potential effects on the spectrophotometric determination of FA. Precision studies (intra-day and inter-day), together with stability and recovery experiments, confirmed the reliability of the procedure. Tablet recovery results further demonstrated that the method can be applied directly to pharmaceutical formulations without significant interference. Overall, the findings show that this UV–Vis approach provides a practical and reproducible strategy for FA determination, while also allowing the evaluation of interaction-related changes in its electronic absorption behavior.

Keywords

Analytical determination, Folic acid, Molecular interactions, Pharmaceutical analysis, UV-Vis Spectroscopy

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Details

Primary Language

English

Subjects

Instrumental Methods

Journal Section

Research Article

Authors

Muhammet Kemal Deveçeker
0009-0000-3685-3695
Türkiye

Süleyman Adıyaman
0009-0003-4359-1779
Türkiye

Sevda Akay Sazaklioglu ^*
0000-0003-0924-1276
Türkiye

Publication Date

October 27, 2026

Submission Date

March 7, 2026

Acceptance Date

October 17, 2026

Published in Issue

Year 2026 Volume: 47 Number: 5

DOI

https://doi.org/10.17776/csj.1904980

IZ

https://izlik.org/JA32TR76YW

APA

Deveçeker, M. K., Adıyaman, S., & Akay Sazaklioglu, S. (2026). Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy. Cumhuriyet Science Journal, 47(5), 973-980. https://doi.org/10.17776/csj.1904980

AMA

1.Deveçeker MK, Adıyaman S, Akay Sazaklioglu S. Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy. CSJ. 2026;47(5):973-980. doi:10.17776/csj.1904980

Chicago

Deveçeker, Muhammet Kemal, Süleyman Adıyaman, and Sevda Akay Sazaklioglu. 2026. “Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy”. Cumhuriyet Science Journal 47 (5): 973-80. https://doi.org/10.17776/csj.1904980.

EndNote

Deveçeker MK, Adıyaman S, Akay Sazaklioglu S (October 1, 2026) Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy. Cumhuriyet Science Journal 47 5 973–980.

IEEE

[1]M. K. Deveçeker, S. Adıyaman, and S. Akay Sazaklioglu, “Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy”, CSJ, vol. 47, no. 5, pp. 973–980, Oct. 2026, doi: 10.17776/csj.1904980.

ISNAD

Deveçeker, Muhammet Kemal - Adıyaman, Süleyman - Akay Sazaklioglu, Sevda. “Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy”. Cumhuriyet Science Journal 47/5 (October 1, 2026): 973-980. https://doi.org/10.17776/csj.1904980.

JAMA

1.Deveçeker MK, Adıyaman S, Akay Sazaklioglu S. Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy. CSJ. 2026;47:973–980.

MLA

Deveçeker, Muhammet Kemal, et al. “Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy”. Cumhuriyet Science Journal, vol. 47, no. 5, Oct. 2026, pp. 973-80, doi:10.17776/csj.1904980.

Vancouver

1.Muhammet Kemal Deveçeker, Süleyman Adıyaman, Sevda Akay Sazaklioglu. Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy. CSJ. 2026 Oct. 1;47(5):973-80. doi:10.17776/csj.1904980

As of 2026, Cumhuriyet Science Journal will be published in six issues per year, released in February, April, June, August, October, and December

Determination and Molecular Interaction Studies of Folic Acid by UV-Vis Spectroscopy

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

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