Investigation of the Antioxidant and DNA Protective Activities of Euphorbia sp. (Spurge) by Different Methods
Abstract
Spurge plants (Euphorbia sp.) are monoecious annual, biennial, or perennial herbs or shrubs with a milky latex sap that have long attracted botanical, chemical, and pharmaceutical interest. In Türkiye several Euphorbia species have been examined for chemical composition and for antioxidant or anticancer properties, but the biological activities of spurge material from the Sivas region had not previously been reported. In the present study, a Euphorbia sp. specimen was collected from the Sivas Cumhuriyet University campus area (Sivas, Türkiye) between May and August 2022. Taxonomic identification was carried out to the genus level only by Prof. Dr. Hüseyin Aşkın Akpulat (Department of Biology); no species-level determination or herbarium voucher was obtained. Stem material was extracted with ethanol, water, methanol, and ethyl acetate, and the antioxidant capacity and plasmid-DNA protective activity of the extracts were examined in vitro using DPPH radical scavenging, total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) assays, together with a pBR322 plasmid-DNA damage-protection assessment. The ethanol extract showed the highest radical-scavenging activity (IC50 = 15.89 μg/mL) and the highest TAS value, whereas the plasmid-DNA protective activity of all extracts was weak under the assay conditions. Because the findings derive from a single genus-level specimen and one tissue, they are presented as a preliminary regional screening that may guide subsequent fractionation and phytochemical work rather than as species-general conclusions.
Keywords
Antioxidant, Biological activity, DNA, Euphorbia, Spurge
Supporting Institution
References
- Arıkan, H. (2019). Pharmacognostic studies on the plant Inula graveolens (L.) Desf. [Master's thesis, Anadolu University]. YÖK Ulusal Tez Merkezi.
- Dündar, E. (2017). Pharmacognostic investigations on the plant Centaurea babylonica L. [Master's thesis, Anadolu University]. YÖK Ulusal Tez Merkezi.
- Selamoglu, Z. (2023). Anti-inflammatory influences of royal jelly and melittin and their effectiveness on wound healing. Central Asian Journal of Medical and Pharmaceutical Sciences Innovation, 3(2), 38–47. https://doi.org/10.22034/CAJMPSI.2023.02.02
- Selamoğlu, Z., Üstüntaş, H. E., & Özgen, S. (2016). Traditional and complementary alternative medicine practices of some aromatic plants in the human health. Research Journal of Biology, 4, 52–54.
- Yıldız, İ. (2019). Investigation of antimicrobial and antioxidant properties of some plant species growing in Turkey [Master's thesis, Anadolu University]. YÖK Ulusal Tez Merkezi.
- Mohammed, F. S., Akgul, H., Sevindik, M., & Khaled, B. M. T. (2018). Phenolic content and biological activities of Rhus coriaria var. Zebaria. Fresenius Environmental Bulletin, 27(8), 5694–5702.
- Nageen, B., Rasul, A., Hussain, G., Shah, M. A., Anwar, H., Hussain, S. M., & Selamoglu, Z. (2021). Jaceosidin: A natural flavone with versatile pharmacological and biological activities. Current Pharmaceutical Design, 27(4), 456–466. https://doi.org/10.2174/1381612826666200429095101
- Selamoğlu, Z. (2017). Polyphenolic compounds in human health with pharmacological properties. Journal of Traditional Medicine & Clinical Naturopathy, 6(4), Article e138. https://doi.org/10.4172/2573-4555.1000e138
- Sevindik, M., Akgul, H., Akata, I., Alli, H., & Selamoglu, Z. (2017). Fomitopsis pinicola in healthful dietary approach and their therapeutic potentials. Acta Alimentaria, 46(4), 464–469. https://doi.org/10.1556/066.2017.46.4.9
- Alinia-Ahandani, E., Hajipour, S., Ghonoudi, A., & Selamoglu, Z. (2026). The effect of phytochemicals on the suppression of cancer metastasis. In A. Zarrabi, A. Khosravi, & A. Zarepour (Eds.), Nanophytomedicine (pp. 101–112). Academic Press. https://doi.org/10.1016/B978-0-443-32888-6.00017-7